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Green Tea Polyphenols and Sulfasalazine have Parallel Anti-Inflammatory Properties in Colitis Models.

Oz HS, Chen T, de Villiers WJ - Front Immunol (2013)

Bottom Line: The inflammatory markers TNFα (3-fold), IL-6 (14-fold), and serum amyloid A (40-fold) increased in colitic animals and significantly decreased with treatment regiments.EGCG additionally reduced leptin levels (p < 0.01) while GrTP and sulfasalazine had no effect on leptin levels (p < 0.05).GrTP and EGCG improved antioxidants levels and attenuated severity of colitis analogous to sulfasalazine.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, University of Kentucky Medical Center , Lexington, KY , USA.

ABSTRACT

Background: There is no cure for autoimmune chronic inflammatory bowel disease (IBD). IBD patients commonly use complementary and alternative medications of which the safety, efficacy, and interaction with standard-of-care therapies are not fully known. Thus the consequences can become life-threatening. Sulfasalazine commonly used in IBD, potentially has severe adverse effects, including infertility, pulmonary fibrosis, lack of response, and ultimately patients may require intestinal resection. We hypothesized that green tea polyphenols (GrTP, EGCG) and sulfasalazine have similar anti-inflammatory properties.

Methods: BALB/c mice received Dextran sodium sulfate (DSS) to induce colitis (ulcerative colitis model). Exposure of IL-10 deficient mice (BALB/c-background) to normal microbiota provoked enterocolitis (mimics Crohn's disease). Animals were treated with agents incorporated into daily diets. Control animals received sham treatment.

Results: DSS-treated animals developed severe bloody diarrhea and colitis (score 0-4, 3.2 ± 0.27). IL-10 deficient mice developed severe enterocolitis as manifested by diarrhea, rectal prolapse, and colonic lesions. Animals tolerated regimens (GrTP, EGCG, sulfasalazine) with no major side effects, and further developed less severe colitis. IL-10 deficient animals became moribund on high dose, while tolerated low and Mid doses with significant improved symptoms of enterocolitis. GrTP, EGCG, and sulfasalazine significantly ameliorated colonic damage and histological scores in treated animals in a similar manner (GrTP vs. DSS p < 0.05; EGCG, sulfasalazine vs. DSS p < 0.01). The inflammatory markers TNFα (3-fold), IL-6 (14-fold), and serum amyloid A (40-fold) increased in colitic animals and significantly decreased with treatment regiments. In contrast, circulatory leptin levels decreased in colitic animals (twofold). EGCG additionally reduced leptin levels (p < 0.01) while GrTP and sulfasalazine had no effect on leptin levels (p < 0.05). Hepatic and colonic antioxidants were significantly depleted in colitic animals and treatment regiments significantly restored antioxidants levels.

Conclusion: GrTP and EGCG improved antioxidants levels and attenuated severity of colitis analogous to sulfasalazine. Future studies will reveal whether polyphenols can become an alternative/additive therapy for IBD therapy in humans.

No MeSH data available.


Related in: MedlinePlus

Circulating leptin level significantly decreased in DSS-induced colitic animals (p < 0.05) and EGCG further reduced the leptin levels (DSS vs. EGCG < 0.05), while GrTP and sulfasalazine had no significant effect on leptin levels (vs. DSS > 0.05).
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Figure 3: Circulating leptin level significantly decreased in DSS-induced colitic animals (p < 0.05) and EGCG further reduced the leptin levels (DSS vs. EGCG < 0.05), while GrTP and sulfasalazine had no significant effect on leptin levels (vs. DSS > 0.05).

Mentions: Leptin production, the marker of satiety, energy and expenditure, with central role in inflammatory response and immune defense was drastically decreased in colitic animals (p < 0.05) and EGCG consumption further reduced the leptin levels while GrTP and sulfasalazine had no additional affect on leptin regulation (p < 0.01) (Figure 3).


Green Tea Polyphenols and Sulfasalazine have Parallel Anti-Inflammatory Properties in Colitis Models.

Oz HS, Chen T, de Villiers WJ - Front Immunol (2013)

Circulating leptin level significantly decreased in DSS-induced colitic animals (p < 0.05) and EGCG further reduced the leptin levels (DSS vs. EGCG < 0.05), while GrTP and sulfasalazine had no significant effect on leptin levels (vs. DSS > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3672863&req=5

Figure 3: Circulating leptin level significantly decreased in DSS-induced colitic animals (p < 0.05) and EGCG further reduced the leptin levels (DSS vs. EGCG < 0.05), while GrTP and sulfasalazine had no significant effect on leptin levels (vs. DSS > 0.05).
Mentions: Leptin production, the marker of satiety, energy and expenditure, with central role in inflammatory response and immune defense was drastically decreased in colitic animals (p < 0.05) and EGCG consumption further reduced the leptin levels while GrTP and sulfasalazine had no additional affect on leptin regulation (p < 0.01) (Figure 3).

Bottom Line: The inflammatory markers TNFα (3-fold), IL-6 (14-fold), and serum amyloid A (40-fold) increased in colitic animals and significantly decreased with treatment regiments.EGCG additionally reduced leptin levels (p < 0.01) while GrTP and sulfasalazine had no effect on leptin levels (p < 0.05).GrTP and EGCG improved antioxidants levels and attenuated severity of colitis analogous to sulfasalazine.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, University of Kentucky Medical Center , Lexington, KY , USA.

ABSTRACT

Background: There is no cure for autoimmune chronic inflammatory bowel disease (IBD). IBD patients commonly use complementary and alternative medications of which the safety, efficacy, and interaction with standard-of-care therapies are not fully known. Thus the consequences can become life-threatening. Sulfasalazine commonly used in IBD, potentially has severe adverse effects, including infertility, pulmonary fibrosis, lack of response, and ultimately patients may require intestinal resection. We hypothesized that green tea polyphenols (GrTP, EGCG) and sulfasalazine have similar anti-inflammatory properties.

Methods: BALB/c mice received Dextran sodium sulfate (DSS) to induce colitis (ulcerative colitis model). Exposure of IL-10 deficient mice (BALB/c-background) to normal microbiota provoked enterocolitis (mimics Crohn's disease). Animals were treated with agents incorporated into daily diets. Control animals received sham treatment.

Results: DSS-treated animals developed severe bloody diarrhea and colitis (score 0-4, 3.2 ± 0.27). IL-10 deficient mice developed severe enterocolitis as manifested by diarrhea, rectal prolapse, and colonic lesions. Animals tolerated regimens (GrTP, EGCG, sulfasalazine) with no major side effects, and further developed less severe colitis. IL-10 deficient animals became moribund on high dose, while tolerated low and Mid doses with significant improved symptoms of enterocolitis. GrTP, EGCG, and sulfasalazine significantly ameliorated colonic damage and histological scores in treated animals in a similar manner (GrTP vs. DSS p < 0.05; EGCG, sulfasalazine vs. DSS p < 0.01). The inflammatory markers TNFα (3-fold), IL-6 (14-fold), and serum amyloid A (40-fold) increased in colitic animals and significantly decreased with treatment regiments. In contrast, circulatory leptin levels decreased in colitic animals (twofold). EGCG additionally reduced leptin levels (p < 0.01) while GrTP and sulfasalazine had no effect on leptin levels (p < 0.05). Hepatic and colonic antioxidants were significantly depleted in colitic animals and treatment regiments significantly restored antioxidants levels.

Conclusion: GrTP and EGCG improved antioxidants levels and attenuated severity of colitis analogous to sulfasalazine. Future studies will reveal whether polyphenols can become an alternative/additive therapy for IBD therapy in humans.

No MeSH data available.


Related in: MedlinePlus