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Periodic knee injections of collagen tripeptide delay cartilage degeneration in rabbit experimental osteoarthritis.

Naraoka T, Ishibashi Y, Tsuda E, Yamamoto Y, Kusumi T, Toh S - Arthritis Res. Ther. (2013)

Bottom Line: A Tukey's honestly significant difference test was used to evaluate the statistical significance of difference in the macroscopic, histological and immnohistochemical results.Histologically, intra-articular injection of Ctp significantly reduced cartilage degradation 10 weeks after surgery, and Ctp/HA significantly reduced it 5 weeks after surgery in comparison with the control.This effect appears to be exerted by promotion of type II collagen synthesis predominantly.

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ABSTRACT

Introduction: Collagen peptides have been reported to possess various biological activities for various cell types. The purposes of this study were, first, to examine the therapeutic effects of collagen tripeptide (Ctp) in rabbit osteoarthritis and, second, to explore a synergetic effect with hyaluronan (HA).

Methods: Osteoarthritis was induced by anterior cruciate ligament transection of the right knee in 72 Japanese white rabbits and they were divided into four groups (control, Ctp, HA and Ctp/HA). Each material was injected weekly into the knee, and knee joint samples were collected 5, 10 and 15 weeks after surgery. Macroscopic and histomorphological analyses of cartilage were conducted. Expression of type II collagen and matrix metalloproteinase-13 was also analyzed immunohistochemically. A Tukey's honestly significant difference test was used to evaluate the statistical significance of difference in the macroscopic, histological and immnohistochemical results.

Results: All treatment groups exhibited slightly higher resistance to the progression of osteoarthritis than the control group macroscopically 15 weeks after surgery. Histologically, intra-articular injection of Ctp significantly reduced cartilage degradation 10 weeks after surgery, and Ctp/HA significantly reduced it 5 weeks after surgery in comparison with the control. Immunohistochemically, both Ctp-treated and Ctp/HA-treated groups had significantly increased type II collagen-positive chondrocytes at the fifth week after the surgery, although the numbers of matrix metalloproteinase-13-positive chondrocytes were not affected.

Conclusion: Periodical injections of Ctp and Ctp/HA delayed progression of cartilage degeneration of early osteoarthritis induced by anterior cruciate ligament transection in rabbits. This effect appears to be exerted by promotion of type II collagen synthesis predominantly.

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Type II collagen expression during osteoarthritis development. Immunohistochemical findings for type II collagen in the medial femoral condylar cartilage from (A) a control knee, (B) a collagen tripeptide (Ctp)-treated knee, (C) a hyaluronan (HA)-treated knee and (D) a Ctp/HA-treated knee 5 weeks after surgery (original magnification ×400). (E) Appearance of type II collagen (×400) staining in the medial femoral condylar cartilage from a normal rabbit knee. (F) Population of type II collagen-positive chondrocytes in medial femoral condylar cartilage of each group 5, 10 and 15 weeks after surgery. Counted area was 6.76 × 104 μm2. Data presented as mean ± standard deviation (*P <0.05).
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Figure 3: Type II collagen expression during osteoarthritis development. Immunohistochemical findings for type II collagen in the medial femoral condylar cartilage from (A) a control knee, (B) a collagen tripeptide (Ctp)-treated knee, (C) a hyaluronan (HA)-treated knee and (D) a Ctp/HA-treated knee 5 weeks after surgery (original magnification ×400). (E) Appearance of type II collagen (×400) staining in the medial femoral condylar cartilage from a normal rabbit knee. (F) Population of type II collagen-positive chondrocytes in medial femoral condylar cartilage of each group 5, 10 and 15 weeks after surgery. Counted area was 6.76 × 104 μm2. Data presented as mean ± standard deviation (*P <0.05).

Mentions: The expression of type II collagen was comparable between the control group and the HA group 5 weeks after surgery (Figure 3A,C). In both groups, type II collagen was stained in a lower number of chondrocytes. In contrast, many chondrocytes expressed type II collagen in the Ctp or Ctp/HA groups at the same time points (Figure 3B,D), and the number of type II collagen-positive chondrocytes in these groups were significantly increased in comparison with the control or HA groups (Figure 3E).


Periodic knee injections of collagen tripeptide delay cartilage degeneration in rabbit experimental osteoarthritis.

Naraoka T, Ishibashi Y, Tsuda E, Yamamoto Y, Kusumi T, Toh S - Arthritis Res. Ther. (2013)

Type II collagen expression during osteoarthritis development. Immunohistochemical findings for type II collagen in the medial femoral condylar cartilage from (A) a control knee, (B) a collagen tripeptide (Ctp)-treated knee, (C) a hyaluronan (HA)-treated knee and (D) a Ctp/HA-treated knee 5 weeks after surgery (original magnification ×400). (E) Appearance of type II collagen (×400) staining in the medial femoral condylar cartilage from a normal rabbit knee. (F) Population of type II collagen-positive chondrocytes in medial femoral condylar cartilage of each group 5, 10 and 15 weeks after surgery. Counted area was 6.76 × 104 μm2. Data presented as mean ± standard deviation (*P <0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3672813&req=5

Figure 3: Type II collagen expression during osteoarthritis development. Immunohistochemical findings for type II collagen in the medial femoral condylar cartilage from (A) a control knee, (B) a collagen tripeptide (Ctp)-treated knee, (C) a hyaluronan (HA)-treated knee and (D) a Ctp/HA-treated knee 5 weeks after surgery (original magnification ×400). (E) Appearance of type II collagen (×400) staining in the medial femoral condylar cartilage from a normal rabbit knee. (F) Population of type II collagen-positive chondrocytes in medial femoral condylar cartilage of each group 5, 10 and 15 weeks after surgery. Counted area was 6.76 × 104 μm2. Data presented as mean ± standard deviation (*P <0.05).
Mentions: The expression of type II collagen was comparable between the control group and the HA group 5 weeks after surgery (Figure 3A,C). In both groups, type II collagen was stained in a lower number of chondrocytes. In contrast, many chondrocytes expressed type II collagen in the Ctp or Ctp/HA groups at the same time points (Figure 3B,D), and the number of type II collagen-positive chondrocytes in these groups were significantly increased in comparison with the control or HA groups (Figure 3E).

Bottom Line: A Tukey's honestly significant difference test was used to evaluate the statistical significance of difference in the macroscopic, histological and immnohistochemical results.Histologically, intra-articular injection of Ctp significantly reduced cartilage degradation 10 weeks after surgery, and Ctp/HA significantly reduced it 5 weeks after surgery in comparison with the control.This effect appears to be exerted by promotion of type II collagen synthesis predominantly.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Introduction: Collagen peptides have been reported to possess various biological activities for various cell types. The purposes of this study were, first, to examine the therapeutic effects of collagen tripeptide (Ctp) in rabbit osteoarthritis and, second, to explore a synergetic effect with hyaluronan (HA).

Methods: Osteoarthritis was induced by anterior cruciate ligament transection of the right knee in 72 Japanese white rabbits and they were divided into four groups (control, Ctp, HA and Ctp/HA). Each material was injected weekly into the knee, and knee joint samples were collected 5, 10 and 15 weeks after surgery. Macroscopic and histomorphological analyses of cartilage were conducted. Expression of type II collagen and matrix metalloproteinase-13 was also analyzed immunohistochemically. A Tukey's honestly significant difference test was used to evaluate the statistical significance of difference in the macroscopic, histological and immnohistochemical results.

Results: All treatment groups exhibited slightly higher resistance to the progression of osteoarthritis than the control group macroscopically 15 weeks after surgery. Histologically, intra-articular injection of Ctp significantly reduced cartilage degradation 10 weeks after surgery, and Ctp/HA significantly reduced it 5 weeks after surgery in comparison with the control. Immunohistochemically, both Ctp-treated and Ctp/HA-treated groups had significantly increased type II collagen-positive chondrocytes at the fifth week after the surgery, although the numbers of matrix metalloproteinase-13-positive chondrocytes were not affected.

Conclusion: Periodical injections of Ctp and Ctp/HA delayed progression of cartilage degeneration of early osteoarthritis induced by anterior cruciate ligament transection in rabbits. This effect appears to be exerted by promotion of type II collagen synthesis predominantly.

Show MeSH
Related in: MedlinePlus