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Expression of regulatory receptors on γδ T cells and their cytokine production in Behcet's disease.

Parlakgul G, Guney E, Erer B, Kılıcaslan Z, Direskeneli H, Gul A, Saruhan-Direskeneli G - Arthritis Res. Ther. (2013)

Bottom Line: NKG2C+ γδ+ T cells were relatively increased (0.5 and 0.6 vs. 0.3%, P=0.008 and 0.018), whereas NKG2D positivity was decreased in patients with BD and TB (77.7 and 75.8 vs. 87.5%, P=0.001 and 0.004).Expansion capacity of γδ T cells in BD and TB as well as production of IL-13, IFN-γ, granulocyte monocyte colony stimulating factor (GM-CSF), TNF-α, CCL4 and CCL5 in BD was lower compared to HC, when restimulated by TLR3 ligand and BrHPP.The changes on γδ T cells of BD as well as TB patients implicate that γδ T cells have already been exposed to regulatory effects, which changed their activity.

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ABSTRACT

Introduction: Behcet's disease (BD) is a multi-systemic disorder with muco-cutaneous, ocular, arthritic, vascular or central nervous system involvement. The role of γδ T cells is implicated in BD. The activation status of γδ T cells and their cytokine secretion against phosphoantigens are evaluated in BD.

Methods: NKG2A, NKG2C, NKG2D, CD16 and CCR7 molecules on γδ T cells were analyzed in 70 BD, 27 tuberculosis (TB) patients and 26 healthy controls (HC). Peripheral γδ T cells were expanded with a phosphoantigen (BrHPP) and IL-2, restimulated with BrHPP and a TLR3 ligand, and cytokine production was measured.

Results: γδ T cells were not increased in both BD and TB patients, but the proportions of TCRVδ2+ T cells were lower (58.9 and 50.7 vs. 71.7%, P=0.04 and P=0.005) compared to HC. Higher proportion of TCRVδ2+ T cells were CD16+ (26.2 and 33.9 vs. 16.6%, P=0.02 and P=0.001) and CCR7- (32.2 and 27.9 vs. 17.7%, P<0.0001 and P=0.014) in BD and TB patients compared to HC. NKG2C+ γδ+ T cells were relatively increased (0.5 and 0.6 vs. 0.3%, P=0.008 and 0.018), whereas NKG2D positivity was decreased in patients with BD and TB (77.7 and 75.8 vs. 87.5%, P=0.001 and 0.004). Expansion capacity of γδ T cells in BD and TB as well as production of IL-13, IFN-γ, granulocyte monocyte colony stimulating factor (GM-CSF), TNF-α, CCL4 and CCL5 in BD was lower compared to HC, when restimulated by TLR3 ligand and BrHPP.

Conclusion: The changes on γδ T cells of BD as well as TB patients implicate that γδ T cells have already been exposed to regulatory effects, which changed their activity. Lower cytokine response of γδ T cells implicates down modulation of these cells in BD.

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TCR γδ+ T cells carrying NKG2D. The % of TCR γδ+ T cells carrying NKG2D in the peripheral blood of 70 BD (38 A-BD, 32 IA-BD), 27 TB patients and 30 HC. The differences between BD and TB to HC were significant (P = 0.001 and P = 0.004).
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Figure 2: TCR γδ+ T cells carrying NKG2D. The % of TCR γδ+ T cells carrying NKG2D in the peripheral blood of 70 BD (38 A-BD, 32 IA-BD), 27 TB patients and 30 HC. The differences between BD and TB to HC were significant (P = 0.001 and P = 0.004).

Mentions: TCRVγ9Vδ2+ T cells can also be activated through NKG2D independently of antigen [21] or by ligands of NKG2D, that is, MICA or MICB, which are often induced by stress [22]. NKG2D receptors on the γδ T cells, however, were decreased in patients with BD and TB compared to HC (77.7 and 75.8 vs. 87.5%, P = 0.001 and 0.004, Figure 2).


Expression of regulatory receptors on γδ T cells and their cytokine production in Behcet's disease.

Parlakgul G, Guney E, Erer B, Kılıcaslan Z, Direskeneli H, Gul A, Saruhan-Direskeneli G - Arthritis Res. Ther. (2013)

TCR γδ+ T cells carrying NKG2D. The % of TCR γδ+ T cells carrying NKG2D in the peripheral blood of 70 BD (38 A-BD, 32 IA-BD), 27 TB patients and 30 HC. The differences between BD and TB to HC were significant (P = 0.001 and P = 0.004).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3672743&req=5

Figure 2: TCR γδ+ T cells carrying NKG2D. The % of TCR γδ+ T cells carrying NKG2D in the peripheral blood of 70 BD (38 A-BD, 32 IA-BD), 27 TB patients and 30 HC. The differences between BD and TB to HC were significant (P = 0.001 and P = 0.004).
Mentions: TCRVγ9Vδ2+ T cells can also be activated through NKG2D independently of antigen [21] or by ligands of NKG2D, that is, MICA or MICB, which are often induced by stress [22]. NKG2D receptors on the γδ T cells, however, were decreased in patients with BD and TB compared to HC (77.7 and 75.8 vs. 87.5%, P = 0.001 and 0.004, Figure 2).

Bottom Line: NKG2C+ γδ+ T cells were relatively increased (0.5 and 0.6 vs. 0.3%, P=0.008 and 0.018), whereas NKG2D positivity was decreased in patients with BD and TB (77.7 and 75.8 vs. 87.5%, P=0.001 and 0.004).Expansion capacity of γδ T cells in BD and TB as well as production of IL-13, IFN-γ, granulocyte monocyte colony stimulating factor (GM-CSF), TNF-α, CCL4 and CCL5 in BD was lower compared to HC, when restimulated by TLR3 ligand and BrHPP.The changes on γδ T cells of BD as well as TB patients implicate that γδ T cells have already been exposed to regulatory effects, which changed their activity.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Introduction: Behcet's disease (BD) is a multi-systemic disorder with muco-cutaneous, ocular, arthritic, vascular or central nervous system involvement. The role of γδ T cells is implicated in BD. The activation status of γδ T cells and their cytokine secretion against phosphoantigens are evaluated in BD.

Methods: NKG2A, NKG2C, NKG2D, CD16 and CCR7 molecules on γδ T cells were analyzed in 70 BD, 27 tuberculosis (TB) patients and 26 healthy controls (HC). Peripheral γδ T cells were expanded with a phosphoantigen (BrHPP) and IL-2, restimulated with BrHPP and a TLR3 ligand, and cytokine production was measured.

Results: γδ T cells were not increased in both BD and TB patients, but the proportions of TCRVδ2+ T cells were lower (58.9 and 50.7 vs. 71.7%, P=0.04 and P=0.005) compared to HC. Higher proportion of TCRVδ2+ T cells were CD16+ (26.2 and 33.9 vs. 16.6%, P=0.02 and P=0.001) and CCR7- (32.2 and 27.9 vs. 17.7%, P<0.0001 and P=0.014) in BD and TB patients compared to HC. NKG2C+ γδ+ T cells were relatively increased (0.5 and 0.6 vs. 0.3%, P=0.008 and 0.018), whereas NKG2D positivity was decreased in patients with BD and TB (77.7 and 75.8 vs. 87.5%, P=0.001 and 0.004). Expansion capacity of γδ T cells in BD and TB as well as production of IL-13, IFN-γ, granulocyte monocyte colony stimulating factor (GM-CSF), TNF-α, CCL4 and CCL5 in BD was lower compared to HC, when restimulated by TLR3 ligand and BrHPP.

Conclusion: The changes on γδ T cells of BD as well as TB patients implicate that γδ T cells have already been exposed to regulatory effects, which changed their activity. Lower cytokine response of γδ T cells implicates down modulation of these cells in BD.

Show MeSH
Related in: MedlinePlus