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OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population.

Jiang Y, Yao M, Liu Q, Zhou C - Arthritis Res. Ther. (2013)

Bottom Line: We found the polymorphisms of the -443C/T and the -66/T/G were significantly associated with the OA risk and the radiographic severity.The -443TT and -66GG showed protective effect against developing OA and were associated with lower Kellgren-Lawrence grade.Besides, the polymorphisms of -443C/T and -66T/G significantly affected the thrombin-cleaved OPN levels in SF from OA subjects.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Introduction: A body of studies suggests the role of osteopontin (OPN) in onset and development of osteoarthritis (OA), however, the association between OPN polymorphisms and OA susceptibility as well as its clinical features has not been reported.

Methods: A total of 750 patients with primary knee OA and 794 healthy volunteer were enrolled as controls. Both OA and control groups were interviewed to obtain demographic and clinical data. Three polymorphisms of OPN gene, namely, -156GG/G, -443C/T and -66T/G were determined. The levels of the full length and the thrombin-cleaved OPN in synovial fluid (SF) from OA subjects were measured.

Results: We found the polymorphisms of the -443C/T and the -66/T/G were significantly associated with the OA risk and the radiographic severity. The -443TT and -66GG showed protective effect against developing OA and were associated with lower Kellgren-Lawrence grade. Besides, the polymorphisms of -443C/T and -66T/G significantly affected the thrombin-cleaved OPN levels in SF from OA subjects. Subjects with -443TT and -66GG genotypes had lower thrombin-cleaved OPN levels in SF. The thrombin-cleaved OPN levels in SF were positively correlated to the radiographic severity of OA.

Conclusions: Our findings suggest that certain OPN gene polymorphisms may be used as molecular markers for the susceptibility and severity of OA.

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Related in: MedlinePlus

Thrombin-cleaved osteopontin (OPN) levels in synovial fluid (SF) from different genotype carriers. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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Figure 3: Thrombin-cleaved osteopontin (OPN) levels in synovial fluid (SF) from different genotype carriers. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Mentions: We next analyzed the thrombin-cleaved OPN levels in SF according to the genotypes of the OPN gene polymorphisms. Our results showed that the thrombin-cleaved OPN levels in SF were significantly lower in -443TT carriers compared with the -443TC and -443CC carriers (4,728 ± 354 vs. 5,398 ± 354 and 5334 ± 323, pg/ml, both P < 0.05). Similarly, the thrombin-cleaved OPN levels in SF from -66GG were lower than from the -66GT and -66TT carriers (4,989 ± 329 vs. 5,413 ± 357 and 5,426 ± 274, pg/ml, both P < 0.05). The genetic polymorphisms of -156G/GG did not influence the thrombin-cleaved OPN levels in SF (Figure 2). Western blot results showed that the thrombin-cleaved OPN expressions in the SF from -443TT patients were markedly lower than that from the -443TC and -443CC patients. The thrombin-cleaved OPN expressions in the SF from -66 GG patients were lower than those from -66GT and -66TT carriers (Figure 3). The protein expression of full-length OPN levels in SF were similar among -443TT carriers compared with the -443TC and -443CC, as well as the -66GG, -66GT and -66TT genotype carriers (figure not shown).


OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population.

Jiang Y, Yao M, Liu Q, Zhou C - Arthritis Res. Ther. (2013)

Thrombin-cleaved osteopontin (OPN) levels in synovial fluid (SF) from different genotype carriers. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3672660&req=5

Figure 3: Thrombin-cleaved osteopontin (OPN) levels in synovial fluid (SF) from different genotype carriers. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Mentions: We next analyzed the thrombin-cleaved OPN levels in SF according to the genotypes of the OPN gene polymorphisms. Our results showed that the thrombin-cleaved OPN levels in SF were significantly lower in -443TT carriers compared with the -443TC and -443CC carriers (4,728 ± 354 vs. 5,398 ± 354 and 5334 ± 323, pg/ml, both P < 0.05). Similarly, the thrombin-cleaved OPN levels in SF from -66GG were lower than from the -66GT and -66TT carriers (4,989 ± 329 vs. 5,413 ± 357 and 5,426 ± 274, pg/ml, both P < 0.05). The genetic polymorphisms of -156G/GG did not influence the thrombin-cleaved OPN levels in SF (Figure 2). Western blot results showed that the thrombin-cleaved OPN expressions in the SF from -443TT patients were markedly lower than that from the -443TC and -443CC patients. The thrombin-cleaved OPN expressions in the SF from -66 GG patients were lower than those from -66GT and -66TT carriers (Figure 3). The protein expression of full-length OPN levels in SF were similar among -443TT carriers compared with the -443TC and -443CC, as well as the -66GG, -66GT and -66TT genotype carriers (figure not shown).

Bottom Line: We found the polymorphisms of the -443C/T and the -66/T/G were significantly associated with the OA risk and the radiographic severity.The -443TT and -66GG showed protective effect against developing OA and were associated with lower Kellgren-Lawrence grade.Besides, the polymorphisms of -443C/T and -66T/G significantly affected the thrombin-cleaved OPN levels in SF from OA subjects.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Introduction: A body of studies suggests the role of osteopontin (OPN) in onset and development of osteoarthritis (OA), however, the association between OPN polymorphisms and OA susceptibility as well as its clinical features has not been reported.

Methods: A total of 750 patients with primary knee OA and 794 healthy volunteer were enrolled as controls. Both OA and control groups were interviewed to obtain demographic and clinical data. Three polymorphisms of OPN gene, namely, -156GG/G, -443C/T and -66T/G were determined. The levels of the full length and the thrombin-cleaved OPN in synovial fluid (SF) from OA subjects were measured.

Results: We found the polymorphisms of the -443C/T and the -66/T/G were significantly associated with the OA risk and the radiographic severity. The -443TT and -66GG showed protective effect against developing OA and were associated with lower Kellgren-Lawrence grade. Besides, the polymorphisms of -443C/T and -66T/G significantly affected the thrombin-cleaved OPN levels in SF from OA subjects. Subjects with -443TT and -66GG genotypes had lower thrombin-cleaved OPN levels in SF. The thrombin-cleaved OPN levels in SF were positively correlated to the radiographic severity of OA.

Conclusions: Our findings suggest that certain OPN gene polymorphisms may be used as molecular markers for the susceptibility and severity of OA.

Show MeSH
Related in: MedlinePlus