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Whole blood impedance aggregometry findings in experimental endotoxinemia.

Simm V, Dirkmann D, Peters J, Hartmann M - Crit Care (2013)

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Effect of lipopolysaccharide (LPS) on the platelet function induced by various activators. After 240 minutes of incubation with LPS (100 μg/mL) or vehicle, heparinized whole blood samples were subjected to impedance aggregometry. The activators - collagen, adenosine diphosphate (ADP), thrombin receptor-activating peptide (TRAP), and arachidonic acid (AA) - were used. Box plots derived from eight experiments are shown. For statistical evaluation, the Wilcoxon test was used.
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Figure 1: Effect of lipopolysaccharide (LPS) on the platelet function induced by various activators. After 240 minutes of incubation with LPS (100 μg/mL) or vehicle, heparinized whole blood samples were subjected to impedance aggregometry. The activators - collagen, adenosine diphosphate (ADP), thrombin receptor-activating peptide (TRAP), and arachidonic acid (AA) - were used. Box plots derived from eight experiments are shown. For statistical evaluation, the Wilcoxon test was used.

Mentions: Incubation of whole blood samples with LPS markedly inhibited platelet function as revealed by determination of the impedance aggregometry variable of area under curve (expressed in arbitrary units). In LPS-treated samples, platelet function was significantly reduced in comparison with vehicle-treated samples when collagen (−55%, P = 0.008), ADP (−45%, P = 0.008), or TRAP (−20%, P = 0.04) served as the activator. In contrast, no LPS-induced decrease in platelet function was detectable when arachidonic acid was used as the activator (Figure 1).


Whole blood impedance aggregometry findings in experimental endotoxinemia.

Simm V, Dirkmann D, Peters J, Hartmann M - Crit Care (2013)

Effect of lipopolysaccharide (LPS) on the platelet function induced by various activators. After 240 minutes of incubation with LPS (100 μg/mL) or vehicle, heparinized whole blood samples were subjected to impedance aggregometry. The activators - collagen, adenosine diphosphate (ADP), thrombin receptor-activating peptide (TRAP), and arachidonic acid (AA) - were used. Box plots derived from eight experiments are shown. For statistical evaluation, the Wilcoxon test was used.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672490&req=5

Figure 1: Effect of lipopolysaccharide (LPS) on the platelet function induced by various activators. After 240 minutes of incubation with LPS (100 μg/mL) or vehicle, heparinized whole blood samples were subjected to impedance aggregometry. The activators - collagen, adenosine diphosphate (ADP), thrombin receptor-activating peptide (TRAP), and arachidonic acid (AA) - were used. Box plots derived from eight experiments are shown. For statistical evaluation, the Wilcoxon test was used.
Mentions: Incubation of whole blood samples with LPS markedly inhibited platelet function as revealed by determination of the impedance aggregometry variable of area under curve (expressed in arbitrary units). In LPS-treated samples, platelet function was significantly reduced in comparison with vehicle-treated samples when collagen (−55%, P = 0.008), ADP (−45%, P = 0.008), or TRAP (−20%, P = 0.04) served as the activator. In contrast, no LPS-induced decrease in platelet function was detectable when arachidonic acid was used as the activator (Figure 1).

View Article: PubMed Central - HTML - PubMed

Show MeSH
Related in: MedlinePlus