Limits...
Glycyrrhizin protects against porcine endotoxemia through modulation of systemic inflammatory response.

Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H - Crit Care (2013)

Bottom Line: However, the effect of GL on HMGB1 expression in endotoxemia as well as its underlying molecular mechanism remained unclear.GL improved systemic hemodynamics and protected vital organs against porcine endotoxemia through modulation of the systemic inflammatory response.By reducing the serum level and gene expression of HMGB1 and other pro-inflammatory cytokines, GL may become a potential agent for the treatment of sepsis.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Introduction: Glycyrrhizin (GL) was recently found to suppress high-mobility group box 1 (HMGB1)-induced injury by binding directly to it. However, the effect of GL on HMGB1 expression in endotoxemia as well as its underlying molecular mechanism remained unclear.

Methods: Twenty-one pigs were divided into four groups: sham group (n=3), control group (n=6), ethyl pyruvate group (n=6) and glycyrrhizin group (n=6). Pigs were anesthetized, mechanically ventilated, monitored and given a continuous intravenous infusion of lipopolysaccharide (LPS). Twelve hours after the start of the LPS infusion, ethyl pyruvate (30 mg/kg/hr) or glycyrrhizin (1 mg/kg/hr) was administered for 12 hours. Systemic and pulmonary hemodynamics, oxygen exchange, and metabolic status were measured. The concentrations of cytokines in serum and the corresponding gene and protein expressions in tissue samples from liver, lungs, kidneys, small intestine and lymph nodes were measured.

Results: GL maintained the stability of systemic hemodynamics and improved pulmonary oxygen exchange and metabolic status. GL also attenuated organ injury and decreased the serum levels of HMGB1 and other pro-inflammatory cytokines by inhibiting their gene and protein expression.

Conclusions: GL improved systemic hemodynamics and protected vital organs against porcine endotoxemia through modulation of the systemic inflammatory response. By reducing the serum level and gene expression of HMGB1 and other pro-inflammatory cytokines, GL may become a potential agent for the treatment of sepsis.

Show MeSH

Related in: MedlinePlus

Organ injury in each group [hematoxylin and eosin (H&E), yellow bar = 100 μm, white bar = 50 μm]. Lung: A marked infiltration of inflammatory cells and hemorrhage were seen in the control group. The EP and GL groups had slighter leukocyte infiltration and almost no hemorrhage. Liver: The control group was marked by interstitial edema. The EP group had a similar change but to a lesser extent. Kidney: The control group had significant interstitial edema and capillary congestion. The EP and GL groups had slighter edema and almost no capillary congestion. Intestine: Severe edema was observed in the small intestine of the control group. The EP and GL groups had similar change but to a lesser extent. EP, ethyl pyruvate; GL, glycyrrhizin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3672474&req=5

Figure 2: Organ injury in each group [hematoxylin and eosin (H&E), yellow bar = 100 μm, white bar = 50 μm]. Lung: A marked infiltration of inflammatory cells and hemorrhage were seen in the control group. The EP and GL groups had slighter leukocyte infiltration and almost no hemorrhage. Liver: The control group was marked by interstitial edema. The EP group had a similar change but to a lesser extent. Kidney: The control group had significant interstitial edema and capillary congestion. The EP and GL groups had slighter edema and almost no capillary congestion. Intestine: Severe edema was observed in the small intestine of the control group. The EP and GL groups had similar change but to a lesser extent. EP, ethyl pyruvate; GL, glycyrrhizin.

Mentions: Microscopy of lung tissue showed that LPS caused a marked infiltration of inflammatory cells and hemorrhage in the con group compared to the sham group, whereas such changes were obviously attenuated in the GL group (Figure 2, Table 3). In the liver, EP and GL led to significant decreases in interstitial WBCs. The sections of the kidney showed a significant decrease in interstitial edema, leukocyte infiltration and capillary congestion when EP and GL were used. EP and GL also caused a significant decrease of Gruenhagen's spaces, denuded surface and interstitial edema in the small intestine (Figure 2, Table 3). There was no obvious injury in the lymph nodes in each group.


Glycyrrhizin protects against porcine endotoxemia through modulation of systemic inflammatory response.

Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H - Crit Care (2013)

Organ injury in each group [hematoxylin and eosin (H&E), yellow bar = 100 μm, white bar = 50 μm]. Lung: A marked infiltration of inflammatory cells and hemorrhage were seen in the control group. The EP and GL groups had slighter leukocyte infiltration and almost no hemorrhage. Liver: The control group was marked by interstitial edema. The EP group had a similar change but to a lesser extent. Kidney: The control group had significant interstitial edema and capillary congestion. The EP and GL groups had slighter edema and almost no capillary congestion. Intestine: Severe edema was observed in the small intestine of the control group. The EP and GL groups had similar change but to a lesser extent. EP, ethyl pyruvate; GL, glycyrrhizin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3672474&req=5

Figure 2: Organ injury in each group [hematoxylin and eosin (H&E), yellow bar = 100 μm, white bar = 50 μm]. Lung: A marked infiltration of inflammatory cells and hemorrhage were seen in the control group. The EP and GL groups had slighter leukocyte infiltration and almost no hemorrhage. Liver: The control group was marked by interstitial edema. The EP group had a similar change but to a lesser extent. Kidney: The control group had significant interstitial edema and capillary congestion. The EP and GL groups had slighter edema and almost no capillary congestion. Intestine: Severe edema was observed in the small intestine of the control group. The EP and GL groups had similar change but to a lesser extent. EP, ethyl pyruvate; GL, glycyrrhizin.
Mentions: Microscopy of lung tissue showed that LPS caused a marked infiltration of inflammatory cells and hemorrhage in the con group compared to the sham group, whereas such changes were obviously attenuated in the GL group (Figure 2, Table 3). In the liver, EP and GL led to significant decreases in interstitial WBCs. The sections of the kidney showed a significant decrease in interstitial edema, leukocyte infiltration and capillary congestion when EP and GL were used. EP and GL also caused a significant decrease of Gruenhagen's spaces, denuded surface and interstitial edema in the small intestine (Figure 2, Table 3). There was no obvious injury in the lymph nodes in each group.

Bottom Line: However, the effect of GL on HMGB1 expression in endotoxemia as well as its underlying molecular mechanism remained unclear.GL improved systemic hemodynamics and protected vital organs against porcine endotoxemia through modulation of the systemic inflammatory response.By reducing the serum level and gene expression of HMGB1 and other pro-inflammatory cytokines, GL may become a potential agent for the treatment of sepsis.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Introduction: Glycyrrhizin (GL) was recently found to suppress high-mobility group box 1 (HMGB1)-induced injury by binding directly to it. However, the effect of GL on HMGB1 expression in endotoxemia as well as its underlying molecular mechanism remained unclear.

Methods: Twenty-one pigs were divided into four groups: sham group (n=3), control group (n=6), ethyl pyruvate group (n=6) and glycyrrhizin group (n=6). Pigs were anesthetized, mechanically ventilated, monitored and given a continuous intravenous infusion of lipopolysaccharide (LPS). Twelve hours after the start of the LPS infusion, ethyl pyruvate (30 mg/kg/hr) or glycyrrhizin (1 mg/kg/hr) was administered for 12 hours. Systemic and pulmonary hemodynamics, oxygen exchange, and metabolic status were measured. The concentrations of cytokines in serum and the corresponding gene and protein expressions in tissue samples from liver, lungs, kidneys, small intestine and lymph nodes were measured.

Results: GL maintained the stability of systemic hemodynamics and improved pulmonary oxygen exchange and metabolic status. GL also attenuated organ injury and decreased the serum levels of HMGB1 and other pro-inflammatory cytokines by inhibiting their gene and protein expression.

Conclusions: GL improved systemic hemodynamics and protected vital organs against porcine endotoxemia through modulation of the systemic inflammatory response. By reducing the serum level and gene expression of HMGB1 and other pro-inflammatory cytokines, GL may become a potential agent for the treatment of sepsis.

Show MeSH
Related in: MedlinePlus