Limits...
Adaptability and persistence of the emerging pathogen Bordetella petrii.

Zelazny AM, Ding L, Goldberg JB, Mijares LA, Conlan S, Conville PS, Stock F, Ballentine SJ, Olivier KN, Sampaio EP, Murray PR, Holland SM - PLoS ONE (2013)

Bottom Line: Strains were compared genetically, phenotypically and by antibody recognition from the patient and from inoculated mice.Finally, we characterize one strain that was poorly recognized by the patient's antibodies, due to a defect in the lipopolysaccharide O-antigen, and identify a mutation associated with this phenotype.We propose that B. petrii is remarkably adaptable in vivo, providing a possible connection between immune response and bacterial evasion and supporting infection persistence.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA. azelazny@mail.nih.gov

ABSTRACT
The first described, environmentally isolated, Bordetella petrii was shown to undergo massive genomic rearrangements in vitro. More recently, B. petrii was isolated from clinical samples associated with jaw, ear bone, cystic fibrosis and chronic pulmonary disease. However, the in vivo consequences of B. petrii genome plasticity and its pathogenicity remain obscure. B. petrii was identified from four sequential respiratory samples and a post-mortem spleen sample of a woman presenting with bronchiectasis and cavitary lung disease associated with nontuberculous mycobacterial infection. Strains were compared genetically, phenotypically and by antibody recognition from the patient and from inoculated mice. The successive B. petrii strains exhibited differences in growth, antibiotic susceptibility and recognition by the patient's antibodies. Antibodies from mice inoculated with these strains recapitulated the specificity and strain dependent response that was seen with the patient's serum. Finally, we characterize one strain that was poorly recognized by the patient's antibodies, due to a defect in the lipopolysaccharide O-antigen, and identify a mutation associated with this phenotype. We propose that B. petrii is remarkably adaptable in vivo, providing a possible connection between immune response and bacterial evasion and supporting infection persistence.

Show MeSH

Related in: MedlinePlus

Molecular typing and growth curves of serially isolated strains of B. petrii..(A) The DiversiLab Non fermentor typing kit was used for rep-PCR typing of B. petrii using DNA from clinical and reference strains. Amplicons were detected with the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) and data analyzed with the DiversiLab software (version 3.3). Results generated include a dendrogram (left) and virtual gel images (right). (B) Genomic DNA was digested with the restriction endonuclease XbaI and separated by PFGE with a CHEF Mapper system. Asterisks indicate band differences among the patient strains. Ladder: Lambda DNA Ladder 48.5 KB–1 MB kb plugs (Lonza). (C) Growth curves were performed on LB broth at 37°C and growth assessed by colony-forming units (CFU) performed with serially diluted aliquots plated on SBA plates. Graph shows mean and SEM from three experiments. B. petrii 1–5: strains of B. petrii serially isolated from our patient; BAA-461: type strain of B. petrii (ATCC BAA-461); 13363: first described clinical strain of B. petrii (NCTC 13363).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3672207&req=5

pone-0065102-g001: Molecular typing and growth curves of serially isolated strains of B. petrii..(A) The DiversiLab Non fermentor typing kit was used for rep-PCR typing of B. petrii using DNA from clinical and reference strains. Amplicons were detected with the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) and data analyzed with the DiversiLab software (version 3.3). Results generated include a dendrogram (left) and virtual gel images (right). (B) Genomic DNA was digested with the restriction endonuclease XbaI and separated by PFGE with a CHEF Mapper system. Asterisks indicate band differences among the patient strains. Ladder: Lambda DNA Ladder 48.5 KB–1 MB kb plugs (Lonza). (C) Growth curves were performed on LB broth at 37°C and growth assessed by colony-forming units (CFU) performed with serially diluted aliquots plated on SBA plates. Graph shows mean and SEM from three experiments. B. petrii 1–5: strains of B. petrii serially isolated from our patient; BAA-461: type strain of B. petrii (ATCC BAA-461); 13363: first described clinical strain of B. petrii (NCTC 13363).

Mentions: To assess strain relatedness both rep-PCR and PFGE analysis were used. As shown in Fig. 1A, the five serial strains were indistinguishable or very similar to each other by rep-PCR, but different from reference strains B. petrii NCTC 13363 and ATCC BAA-461.


Adaptability and persistence of the emerging pathogen Bordetella petrii.

Zelazny AM, Ding L, Goldberg JB, Mijares LA, Conlan S, Conville PS, Stock F, Ballentine SJ, Olivier KN, Sampaio EP, Murray PR, Holland SM - PLoS ONE (2013)

Molecular typing and growth curves of serially isolated strains of B. petrii..(A) The DiversiLab Non fermentor typing kit was used for rep-PCR typing of B. petrii using DNA from clinical and reference strains. Amplicons were detected with the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) and data analyzed with the DiversiLab software (version 3.3). Results generated include a dendrogram (left) and virtual gel images (right). (B) Genomic DNA was digested with the restriction endonuclease XbaI and separated by PFGE with a CHEF Mapper system. Asterisks indicate band differences among the patient strains. Ladder: Lambda DNA Ladder 48.5 KB–1 MB kb plugs (Lonza). (C) Growth curves were performed on LB broth at 37°C and growth assessed by colony-forming units (CFU) performed with serially diluted aliquots plated on SBA plates. Graph shows mean and SEM from three experiments. B. petrii 1–5: strains of B. petrii serially isolated from our patient; BAA-461: type strain of B. petrii (ATCC BAA-461); 13363: first described clinical strain of B. petrii (NCTC 13363).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672207&req=5

pone-0065102-g001: Molecular typing and growth curves of serially isolated strains of B. petrii..(A) The DiversiLab Non fermentor typing kit was used for rep-PCR typing of B. petrii using DNA from clinical and reference strains. Amplicons were detected with the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) and data analyzed with the DiversiLab software (version 3.3). Results generated include a dendrogram (left) and virtual gel images (right). (B) Genomic DNA was digested with the restriction endonuclease XbaI and separated by PFGE with a CHEF Mapper system. Asterisks indicate band differences among the patient strains. Ladder: Lambda DNA Ladder 48.5 KB–1 MB kb plugs (Lonza). (C) Growth curves were performed on LB broth at 37°C and growth assessed by colony-forming units (CFU) performed with serially diluted aliquots plated on SBA plates. Graph shows mean and SEM from three experiments. B. petrii 1–5: strains of B. petrii serially isolated from our patient; BAA-461: type strain of B. petrii (ATCC BAA-461); 13363: first described clinical strain of B. petrii (NCTC 13363).
Mentions: To assess strain relatedness both rep-PCR and PFGE analysis were used. As shown in Fig. 1A, the five serial strains were indistinguishable or very similar to each other by rep-PCR, but different from reference strains B. petrii NCTC 13363 and ATCC BAA-461.

Bottom Line: Strains were compared genetically, phenotypically and by antibody recognition from the patient and from inoculated mice.Finally, we characterize one strain that was poorly recognized by the patient's antibodies, due to a defect in the lipopolysaccharide O-antigen, and identify a mutation associated with this phenotype.We propose that B. petrii is remarkably adaptable in vivo, providing a possible connection between immune response and bacterial evasion and supporting infection persistence.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA. azelazny@mail.nih.gov

ABSTRACT
The first described, environmentally isolated, Bordetella petrii was shown to undergo massive genomic rearrangements in vitro. More recently, B. petrii was isolated from clinical samples associated with jaw, ear bone, cystic fibrosis and chronic pulmonary disease. However, the in vivo consequences of B. petrii genome plasticity and its pathogenicity remain obscure. B. petrii was identified from four sequential respiratory samples and a post-mortem spleen sample of a woman presenting with bronchiectasis and cavitary lung disease associated with nontuberculous mycobacterial infection. Strains were compared genetically, phenotypically and by antibody recognition from the patient and from inoculated mice. The successive B. petrii strains exhibited differences in growth, antibiotic susceptibility and recognition by the patient's antibodies. Antibodies from mice inoculated with these strains recapitulated the specificity and strain dependent response that was seen with the patient's serum. Finally, we characterize one strain that was poorly recognized by the patient's antibodies, due to a defect in the lipopolysaccharide O-antigen, and identify a mutation associated with this phenotype. We propose that B. petrii is remarkably adaptable in vivo, providing a possible connection between immune response and bacterial evasion and supporting infection persistence.

Show MeSH
Related in: MedlinePlus