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Quantitative trait loci (QTL) associated with resistance to a monogenean parasite (Benedenia seriolae) in yellowtail (Seriola quinqueradiata) through genome wide analysis.

Ozaki A, Yoshida K, Fuji K, Kubota S, Kai W, Aoki JY, Kawabata Y, Suzuki J, Akita K, Koyama T, Nakagawa M, Hotta T, Tsuzaki T, Okamoto N, Araki K, Sakamoto T - PLoS ONE (2013)

Bottom Line: Genetic variation has been inferred to play a significant role in determining the susceptibility to this parasitic disease.These QTL regions explained 32.9-35.5% of the phenotypic variance.The QTL related to growth was found on another linkage group (Squ7).

View Article: PubMed Central - PubMed

Affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Nakatsuhamaura, Minamiise-cho, Watarai-gun, Mie, Japan. aozaki@affrc.go.jp

ABSTRACT
Benedenia infections caused by the monogenean fluke ectoparasite Benedenia seriolae seriously impact marine finfish aquaculture. Genetic variation has been inferred to play a significant role in determining the susceptibility to this parasitic disease. To evaluate the genetic basis of Benedenia disease resistance in yellowtail (Seriola quinqueradiata), a genome-wide and chromosome-wide linkage analyses were initiated using F1 yellowtail families (n = 90 per family) based on a high-density linkage map with 860 microsatellite and 142 single nucleotide polymorphism (SNP) markers. Two major quantitative trait loci (QTL) regions on linkage groups Squ2 (BDR-1) and Squ20 (BDR-2) were identified. These QTL regions explained 32.9-35.5% of the phenotypic variance. On the other hand, we investigated the relationship between QTL for susceptibility to B. seriolae and QTL for fish body size. The QTL related to growth was found on another linkage group (Squ7). As a result, this is the first genetic evidence that contributes to detailing phenotypic resistance to Benedenia disease, and the results will help resolve the mechanism of resistance to this important parasitic infection of yellowtail.

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Related in: MedlinePlus

Simple interval mapping results for Benedenia disease resistance and body weight in all linkage groups with family A.Squ(linkage group)F; marker distance in female map. This figure is described using R/qtl. Number of parasites; Pg <0.05 significant threshold is indicated as solid line. Body weight; Pg <0.05 significant is indicated as dashed line.
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pone-0064987-g001: Simple interval mapping results for Benedenia disease resistance and body weight in all linkage groups with family A.Squ(linkage group)F; marker distance in female map. This figure is described using R/qtl. Number of parasites; Pg <0.05 significant threshold is indicated as solid line. Body weight; Pg <0.05 significant is indicated as dashed line.

Mentions: We show the interval mapping results for Benedenia disease resistance in family A for all linkage groups in Figure 1. Three regions of the chromosomes were identified to be significantly associated with Benedenia desease for family A (Table 3). The QTL at Squ2 identified by simple interval mapping were also found by using K-W test. The peak LOD value of sequ1295BAC (LOD = 4.71) was substantially higher than the genome-wide LOD significance threshold value of 2.9 determined by permutation testing (Pg <0.05; Pg: P value genome-wide LOD). Linkage group in Squ2 QTL region (tentatively termed BDR-1) was observed as a high single peak as genome-wide LOD significance level (Pg <0.001) in interval mapping (Figure 2A). The markers of chromosomal region of Squ8 linkage group, example sequ0670BAC (LOD = 2.45), was less than the genome-wide LOD significance level (Pg <0.05). The markers of chromosomal region of Squ20 linkage group, example Sequ0808TUF (LOD = 2.98), had slightly exceeded the genome-wide LOD significance level (Pg <0.05) (Figure 2B). About one of the peaks, it can tentatively be called as the BDR-2 significant region, based on the rules of QTL nomenclature [13], [14].


Quantitative trait loci (QTL) associated with resistance to a monogenean parasite (Benedenia seriolae) in yellowtail (Seriola quinqueradiata) through genome wide analysis.

Ozaki A, Yoshida K, Fuji K, Kubota S, Kai W, Aoki JY, Kawabata Y, Suzuki J, Akita K, Koyama T, Nakagawa M, Hotta T, Tsuzaki T, Okamoto N, Araki K, Sakamoto T - PLoS ONE (2013)

Simple interval mapping results for Benedenia disease resistance and body weight in all linkage groups with family A.Squ(linkage group)F; marker distance in female map. This figure is described using R/qtl. Number of parasites; Pg <0.05 significant threshold is indicated as solid line. Body weight; Pg <0.05 significant is indicated as dashed line.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672171&req=5

pone-0064987-g001: Simple interval mapping results for Benedenia disease resistance and body weight in all linkage groups with family A.Squ(linkage group)F; marker distance in female map. This figure is described using R/qtl. Number of parasites; Pg <0.05 significant threshold is indicated as solid line. Body weight; Pg <0.05 significant is indicated as dashed line.
Mentions: We show the interval mapping results for Benedenia disease resistance in family A for all linkage groups in Figure 1. Three regions of the chromosomes were identified to be significantly associated with Benedenia desease for family A (Table 3). The QTL at Squ2 identified by simple interval mapping were also found by using K-W test. The peak LOD value of sequ1295BAC (LOD = 4.71) was substantially higher than the genome-wide LOD significance threshold value of 2.9 determined by permutation testing (Pg <0.05; Pg: P value genome-wide LOD). Linkage group in Squ2 QTL region (tentatively termed BDR-1) was observed as a high single peak as genome-wide LOD significance level (Pg <0.001) in interval mapping (Figure 2A). The markers of chromosomal region of Squ8 linkage group, example sequ0670BAC (LOD = 2.45), was less than the genome-wide LOD significance level (Pg <0.05). The markers of chromosomal region of Squ20 linkage group, example Sequ0808TUF (LOD = 2.98), had slightly exceeded the genome-wide LOD significance level (Pg <0.05) (Figure 2B). About one of the peaks, it can tentatively be called as the BDR-2 significant region, based on the rules of QTL nomenclature [13], [14].

Bottom Line: Genetic variation has been inferred to play a significant role in determining the susceptibility to this parasitic disease.These QTL regions explained 32.9-35.5% of the phenotypic variance.The QTL related to growth was found on another linkage group (Squ7).

View Article: PubMed Central - PubMed

Affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Nakatsuhamaura, Minamiise-cho, Watarai-gun, Mie, Japan. aozaki@affrc.go.jp

ABSTRACT
Benedenia infections caused by the monogenean fluke ectoparasite Benedenia seriolae seriously impact marine finfish aquaculture. Genetic variation has been inferred to play a significant role in determining the susceptibility to this parasitic disease. To evaluate the genetic basis of Benedenia disease resistance in yellowtail (Seriola quinqueradiata), a genome-wide and chromosome-wide linkage analyses were initiated using F1 yellowtail families (n = 90 per family) based on a high-density linkage map with 860 microsatellite and 142 single nucleotide polymorphism (SNP) markers. Two major quantitative trait loci (QTL) regions on linkage groups Squ2 (BDR-1) and Squ20 (BDR-2) were identified. These QTL regions explained 32.9-35.5% of the phenotypic variance. On the other hand, we investigated the relationship between QTL for susceptibility to B. seriolae and QTL for fish body size. The QTL related to growth was found on another linkage group (Squ7). As a result, this is the first genetic evidence that contributes to detailing phenotypic resistance to Benedenia disease, and the results will help resolve the mechanism of resistance to this important parasitic infection of yellowtail.

Show MeSH
Related in: MedlinePlus