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A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

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Selenium speciation in different tissues of Cardamine hupingshanesis (Brassicaceae).Top: leaves (n = 16); Middle: stem (n = 15); Bottom: roots (n = 10). The speciation analysis was only conducted when the plant had enough tissues samples of root, stem and leaf.
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pone-0065615-g004: Selenium speciation in different tissues of Cardamine hupingshanesis (Brassicaceae).Top: leaves (n = 16); Middle: stem (n = 15); Bottom: roots (n = 10). The speciation analysis was only conducted when the plant had enough tissues samples of root, stem and leaf.

Mentions: The chemical compositions of Se in C. hupingshanesis are shown in Figure 4, while the Se speciation in L. (Ledeb.) turcz is presented in Table 2. The dominant chemical compounds of Se in C. hupingshanesis included SeCys2 with 78±16% in leaves, 74±17% in stems, and 78±23% in roots, followed by SeMeCys with 12.91±6.74% in leaves, 16.65±9.21% in stems, and 12.43±8.88% in roots, and Se(IV) with 5.12±6.77% in leaves, 4.07±4.31% in stems, and 8.71±10.23% in roots. Moreover, the accumulation of SeCys2 was greater in the plant having higher contents of total Se. The Se speciation in C. hupingshanesis also varied among different sampling sites. At Site 3 (or YTB 3) where the highest plant Se concentrations were observed, SeCys2 accounted for almost all the Se accumulated in C. hupingshanesis. In contrast, the lowest proportion of SeCys2 in C. hupingshanesis was recorded at YTB 8 sampling site, with 30.37% in stem and 38.37% in leaves, which were corresponding to the lowest total Se contents with 128 mg/kg DW in stem and 66.67 mg/kg DW in leaves. Furthermore, there were no significant differences in Se speciation of plant tissues between roots and leaves (Two Sample t Test, t = 0.50, Df = 22, P>0.05), and between stem and leaves (Two Sample t Test, t = 0.64, Df = 27, P>0.05).


A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Selenium speciation in different tissues of Cardamine hupingshanesis (Brassicaceae).Top: leaves (n = 16); Middle: stem (n = 15); Bottom: roots (n = 10). The speciation analysis was only conducted when the plant had enough tissues samples of root, stem and leaf.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3672165&req=5

pone-0065615-g004: Selenium speciation in different tissues of Cardamine hupingshanesis (Brassicaceae).Top: leaves (n = 16); Middle: stem (n = 15); Bottom: roots (n = 10). The speciation analysis was only conducted when the plant had enough tissues samples of root, stem and leaf.
Mentions: The chemical compositions of Se in C. hupingshanesis are shown in Figure 4, while the Se speciation in L. (Ledeb.) turcz is presented in Table 2. The dominant chemical compounds of Se in C. hupingshanesis included SeCys2 with 78±16% in leaves, 74±17% in stems, and 78±23% in roots, followed by SeMeCys with 12.91±6.74% in leaves, 16.65±9.21% in stems, and 12.43±8.88% in roots, and Se(IV) with 5.12±6.77% in leaves, 4.07±4.31% in stems, and 8.71±10.23% in roots. Moreover, the accumulation of SeCys2 was greater in the plant having higher contents of total Se. The Se speciation in C. hupingshanesis also varied among different sampling sites. At Site 3 (or YTB 3) where the highest plant Se concentrations were observed, SeCys2 accounted for almost all the Se accumulated in C. hupingshanesis. In contrast, the lowest proportion of SeCys2 in C. hupingshanesis was recorded at YTB 8 sampling site, with 30.37% in stem and 38.37% in leaves, which were corresponding to the lowest total Se contents with 128 mg/kg DW in stem and 66.67 mg/kg DW in leaves. Furthermore, there were no significant differences in Se speciation of plant tissues between roots and leaves (Two Sample t Test, t = 0.50, Df = 22, P>0.05), and between stem and leaves (Two Sample t Test, t = 0.64, Df = 27, P>0.05).

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

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