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A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

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Total Se content in underlying top sediment of Cardamine hupingshanesis (Brassicaceae).The error bar was calculated on triplicate samples.
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pone-0065615-g003: Total Se content in underlying top sediment of Cardamine hupingshanesis (Brassicaceae).The error bar was calculated on triplicate samples.

Mentions: Total Se concentrations in underlying top sediments (0–3 cm depth) of C. hupingshanesis and L. (Ledeb.) turcz are shown in Figure 3 and Table 2, respectively. The sediments Se concentrations generally varied from 10 to 70 mg/kg DW, except for those sampling sites near Se mine tailings (YTB 3 and 14) where sediment Se concentrations were 274±152 (n = 3) and 177±200 (n = 3) mg/kg DW, respectively. Since these two plants were only co-existed at six sampling sites (YTB 7–9, YTB 11–13), the calculated average sediment Se concentrations on those points were 25.39±14.16 (n = 8) mg/kg DW for C. hupingshanesis and 28.78±14.76 (n = 6) mg/kg DW for L. (Ledeb.) turcz, and there was no significant (K-S Test, D = 0.54, Z = 0.29, P = 0.19) difference between them though the total Se concentrations in C. hupingshanesis tissues were significant higher than those of L. (Ledeb.) turcz tissues.


A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Total Se content in underlying top sediment of Cardamine hupingshanesis (Brassicaceae).The error bar was calculated on triplicate samples.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672165&req=5

pone-0065615-g003: Total Se content in underlying top sediment of Cardamine hupingshanesis (Brassicaceae).The error bar was calculated on triplicate samples.
Mentions: Total Se concentrations in underlying top sediments (0–3 cm depth) of C. hupingshanesis and L. (Ledeb.) turcz are shown in Figure 3 and Table 2, respectively. The sediments Se concentrations generally varied from 10 to 70 mg/kg DW, except for those sampling sites near Se mine tailings (YTB 3 and 14) where sediment Se concentrations were 274±152 (n = 3) and 177±200 (n = 3) mg/kg DW, respectively. Since these two plants were only co-existed at six sampling sites (YTB 7–9, YTB 11–13), the calculated average sediment Se concentrations on those points were 25.39±14.16 (n = 8) mg/kg DW for C. hupingshanesis and 28.78±14.76 (n = 6) mg/kg DW for L. (Ledeb.) turcz, and there was no significant (K-S Test, D = 0.54, Z = 0.29, P = 0.19) difference between them though the total Se concentrations in C. hupingshanesis tissues were significant higher than those of L. (Ledeb.) turcz tissues.

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

Show MeSH