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A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

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Concentrations of total Se in different tissues of Cardamine hupingshanesis (Brassicaceae). Top: leaves; Middle: stem; Bottom: roots. The error bar was calculated on triplicate samples.
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pone-0065615-g002: Concentrations of total Se in different tissues of Cardamine hupingshanesis (Brassicaceae). Top: leaves; Middle: stem; Bottom: roots. The error bar was calculated on triplicate samples.

Mentions: Concentrations of total Se accumulated in plant tissues of C. hupingshanesis are shown in Fig. 2. The highest plant Se accumulation was observed at the sampling site 3 (or YTB-3) in Creek 1 (YTB 1–6 sampling sites, see Fig. 1), showing 1965±271 (n = 3) mg/kg DW in leaves, 1787±167 (n = 3) mg/kg DW in stem, and 4414±3446 (n = 3) mg/kg DW in roots. Relative lower Se concentrations in C. hupingshanesis were found in Creek 2 (YTB 7–11 sampling sites), with 56.85±19.20 mg/kg DW (n = 5) in roots, 132.67±21.48 mg/kg DW (n = 5) in stems, and 117.70±52.84 mg/kg DW (n = 5) in leaves. Concentrations of total Se in L. (Ledeb.) turcz (Steraceae) at YTB 4–6 and 8–10 sampling sites were compiled in Table 2. Concentrations of total Se in L. (Ledeb.) turcz grown in Creeks 1 and 2 (n = 6) were 18.98±8.13 mg/kg in root, 14.01±7.78 mg/kg in stem, and 25.62±18.05 mg/kg in leaves.


A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Concentrations of total Se in different tissues of Cardamine hupingshanesis (Brassicaceae). Top: leaves; Middle: stem; Bottom: roots. The error bar was calculated on triplicate samples.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672165&req=5

pone-0065615-g002: Concentrations of total Se in different tissues of Cardamine hupingshanesis (Brassicaceae). Top: leaves; Middle: stem; Bottom: roots. The error bar was calculated on triplicate samples.
Mentions: Concentrations of total Se accumulated in plant tissues of C. hupingshanesis are shown in Fig. 2. The highest plant Se accumulation was observed at the sampling site 3 (or YTB-3) in Creek 1 (YTB 1–6 sampling sites, see Fig. 1), showing 1965±271 (n = 3) mg/kg DW in leaves, 1787±167 (n = 3) mg/kg DW in stem, and 4414±3446 (n = 3) mg/kg DW in roots. Relative lower Se concentrations in C. hupingshanesis were found in Creek 2 (YTB 7–11 sampling sites), with 56.85±19.20 mg/kg DW (n = 5) in roots, 132.67±21.48 mg/kg DW (n = 5) in stems, and 117.70±52.84 mg/kg DW (n = 5) in leaves. Concentrations of total Se in L. (Ledeb.) turcz (Steraceae) at YTB 4–6 and 8–10 sampling sites were compiled in Table 2. Concentrations of total Se in L. (Ledeb.) turcz grown in Creeks 1 and 2 (n = 6) were 18.98±8.13 mg/kg in root, 14.01±7.78 mg/kg in stem, and 25.62±18.05 mg/kg in leaves.

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

Show MeSH