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A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

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Study site and sampling.A (left): the study area located in Enshi; B (right): 16 sampling sites in selenium mine drainage creeks from Yutangba, Enshi.
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pone-0065615-g001: Study site and sampling.A (left): the study area located in Enshi; B (right): 16 sampling sites in selenium mine drainage creeks from Yutangba, Enshi.

Mentions: Enshi is located in western Hubei province, China (Fig. 1a). It has the only selenium (Se) mines in the world, which were formed during the Maokou, Late Permian period. Carbon-siliceous sediment (also called “stone coal”) contains the highest content of Se (up to 8,500 mg/kg), followed by silicon-carbonaceous sediment and then peat coal [1], [2]. Human activities, such as coal mining and agricultural soil amendment with coal ash, have played an important role in the transport and distribution of Se in the local environment [3]. In particular, those processes have substantially increased the bioavailability of Se in soil-plant systems.


A novel selenocystine-accumulating plant in selenium-mine drainage area in Enshi, China.

Yuan L, Zhu Y, Lin ZQ, Banuelos G, Li W, Yin X - PLoS ONE (2013)

Study site and sampling.A (left): the study area located in Enshi; B (right): 16 sampling sites in selenium mine drainage creeks from Yutangba, Enshi.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672165&req=5

pone-0065615-g001: Study site and sampling.A (left): the study area located in Enshi; B (right): 16 sampling sites in selenium mine drainage creeks from Yutangba, Enshi.
Mentions: Enshi is located in western Hubei province, China (Fig. 1a). It has the only selenium (Se) mines in the world, which were formed during the Maokou, Late Permian period. Carbon-siliceous sediment (also called “stone coal”) contains the highest content of Se (up to 8,500 mg/kg), followed by silicon-carbonaceous sediment and then peat coal [1], [2]. Human activities, such as coal mining and agricultural soil amendment with coal ash, have played an important role in the transport and distribution of Se in the local environment [3]. In particular, those processes have substantially increased the bioavailability of Se in soil-plant systems.

Bottom Line: The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator.Stanleya pinnata).There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Bio-Engineering Research Centre of Selenium, Suzhou, Jiangsu, China ; School of Earth and Space Sciences, University of Science and Technology of China, Hefei, Anhui, China. yuanli@mail.ustc.edu.cn

ABSTRACT
Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239±201 mg/kg DW in roots, 316±184 mg/kg DW in stems, and 380±323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965±271 mg/kg DW in leaves, 1787±167 mg/kg DW in stem and 4414±3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys2), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys2 accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed.

Show MeSH