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Intermedin attenuates LPS-induced inflammation in the rat testis.

Li L, Ma P, Liu Y, Huang C, O WS, Tang F, Zhang JV - PLoS ONE (2013)

Bottom Line: In a rat model, bacterial lippolysaccharide (LPS) induced atypical orchitis, and LPS treatment upregulated the expression of IMD and one of its receptor component proteins, i.e. receptor activity modifying protein 2 (RAMP2).The restorative effect of IMD on steroidogenesis was also observed in hydrogen peroxide-treated rat primary Leydig cells culture.Our results indicate IMD plays an important protective role in spermatogenesis and steroidogenesis, suggesting therapeutic potential for IMD in pathological conditions such as orchitis.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advance Technology, Chinese Academy of Sciences, Shenzhen City, China.

ABSTRACT
First reported as a vasoactive peptide in the cardiovascular system, intermedin (IMD), also known as adrenomedullin 2 (ADM2), is a hormone with multiple potent roles, including its antioxidant action on the pulmonary, central nervous, cardiovascular and renal systems. Though IMD may play certain roles in trophoblast cell invasion, early embryonic development and cumulus cell-oocyte interaction, the role of IMD in the male reproductive system has yet to be investigated. This paper reports our findings on the gene expression of IMD, its receptor components and its protein localization in the testes. In a rat model, bacterial lippolysaccharide (LPS) induced atypical orchitis, and LPS treatment upregulated the expression of IMD and one of its receptor component proteins, i.e. receptor activity modifying protein 2 (RAMP2). IMD decreased both plasma and testicular levels of reactive oxygen species (ROS) production, attenuated the increase in the gene expression of the proinflammatory cytokines tumor necrosis factor alpha (TNFα), interleukin 6 (IL6) and interleukin 1 beta (IL1β), rescued spermatogenesis, and prevented the decrease in plasma testosterone levels caused by LPS. The restorative effect of IMD on steroidogenesis was also observed in hydrogen peroxide-treated rat primary Leydig cells culture. Our results indicate IMD plays an important protective role in spermatogenesis and steroidogenesis, suggesting therapeutic potential for IMD in pathological conditions such as orchitis.

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Related in: MedlinePlus

Gene expression of IMD and its receptor components in rat male reproductive systems.The relative gene expression of intermedin (IMD) and its receptors components CLR, RAMP1, RAMP2 and RAMP3 in adult rat male reproductive systems including testes, epididymis, vas deferens, seminal vesicles, coagulating glands and prostate was analyzed by QPCR. Beta-actin served as the reference gene; all data were expressed as mean ± SEM; n = 5–6.
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pone-0065278-g001: Gene expression of IMD and its receptor components in rat male reproductive systems.The relative gene expression of intermedin (IMD) and its receptors components CLR, RAMP1, RAMP2 and RAMP3 in adult rat male reproductive systems including testes, epididymis, vas deferens, seminal vesicles, coagulating glands and prostate was analyzed by QPCR. Beta-actin served as the reference gene; all data were expressed as mean ± SEM; n = 5–6.

Mentions: QPCR analysis showed the gene expression of IMD and its receptor components in male reproductive system of rats, including the testes (FIG.1). Compared with seminal vehicles and coagulating glands, the gene expression of IMD is relatively low, while those of CLR and RAMP2 are relatively high in the testes. The epididymis also showed a relatively high expression level of RAMP2 and RAMP3. Immunohistochemistry localized IMD in human, mouse and rat testes, both in the interstitial Leydig cells and to a lesser extent in the seminiferous tubules, including the tails of the spermatids (FIG.2). RIA analysis showed that the testes had the highest level of IMD peptide (113.1±19.5 fmol/mg protein) in rats compared with those in the accessory sex glands (Table 1). The high level of IMD and the expression of its receptor components in the testes imply a potentially important biological role.


Intermedin attenuates LPS-induced inflammation in the rat testis.

Li L, Ma P, Liu Y, Huang C, O WS, Tang F, Zhang JV - PLoS ONE (2013)

Gene expression of IMD and its receptor components in rat male reproductive systems.The relative gene expression of intermedin (IMD) and its receptors components CLR, RAMP1, RAMP2 and RAMP3 in adult rat male reproductive systems including testes, epididymis, vas deferens, seminal vesicles, coagulating glands and prostate was analyzed by QPCR. Beta-actin served as the reference gene; all data were expressed as mean ± SEM; n = 5–6.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672160&req=5

pone-0065278-g001: Gene expression of IMD and its receptor components in rat male reproductive systems.The relative gene expression of intermedin (IMD) and its receptors components CLR, RAMP1, RAMP2 and RAMP3 in adult rat male reproductive systems including testes, epididymis, vas deferens, seminal vesicles, coagulating glands and prostate was analyzed by QPCR. Beta-actin served as the reference gene; all data were expressed as mean ± SEM; n = 5–6.
Mentions: QPCR analysis showed the gene expression of IMD and its receptor components in male reproductive system of rats, including the testes (FIG.1). Compared with seminal vehicles and coagulating glands, the gene expression of IMD is relatively low, while those of CLR and RAMP2 are relatively high in the testes. The epididymis also showed a relatively high expression level of RAMP2 and RAMP3. Immunohistochemistry localized IMD in human, mouse and rat testes, both in the interstitial Leydig cells and to a lesser extent in the seminiferous tubules, including the tails of the spermatids (FIG.2). RIA analysis showed that the testes had the highest level of IMD peptide (113.1±19.5 fmol/mg protein) in rats compared with those in the accessory sex glands (Table 1). The high level of IMD and the expression of its receptor components in the testes imply a potentially important biological role.

Bottom Line: In a rat model, bacterial lippolysaccharide (LPS) induced atypical orchitis, and LPS treatment upregulated the expression of IMD and one of its receptor component proteins, i.e. receptor activity modifying protein 2 (RAMP2).The restorative effect of IMD on steroidogenesis was also observed in hydrogen peroxide-treated rat primary Leydig cells culture.Our results indicate IMD plays an important protective role in spermatogenesis and steroidogenesis, suggesting therapeutic potential for IMD in pathological conditions such as orchitis.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advance Technology, Chinese Academy of Sciences, Shenzhen City, China.

ABSTRACT
First reported as a vasoactive peptide in the cardiovascular system, intermedin (IMD), also known as adrenomedullin 2 (ADM2), is a hormone with multiple potent roles, including its antioxidant action on the pulmonary, central nervous, cardiovascular and renal systems. Though IMD may play certain roles in trophoblast cell invasion, early embryonic development and cumulus cell-oocyte interaction, the role of IMD in the male reproductive system has yet to be investigated. This paper reports our findings on the gene expression of IMD, its receptor components and its protein localization in the testes. In a rat model, bacterial lippolysaccharide (LPS) induced atypical orchitis, and LPS treatment upregulated the expression of IMD and one of its receptor component proteins, i.e. receptor activity modifying protein 2 (RAMP2). IMD decreased both plasma and testicular levels of reactive oxygen species (ROS) production, attenuated the increase in the gene expression of the proinflammatory cytokines tumor necrosis factor alpha (TNFα), interleukin 6 (IL6) and interleukin 1 beta (IL1β), rescued spermatogenesis, and prevented the decrease in plasma testosterone levels caused by LPS. The restorative effect of IMD on steroidogenesis was also observed in hydrogen peroxide-treated rat primary Leydig cells culture. Our results indicate IMD plays an important protective role in spermatogenesis and steroidogenesis, suggesting therapeutic potential for IMD in pathological conditions such as orchitis.

Show MeSH
Related in: MedlinePlus