Limits...
Role of murine intestinal interleukin-1 receptor 1-expressing lymphoid tissue inducer-like cells in Salmonella infection.

Chen VL, Surana NK, Duan J, Kasper DL - PLoS ONE (2013)

Bottom Line: These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling.LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium.Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA.

ABSTRACT
Interleukin (IL)-1 signaling plays a critical role in intestinal immunology. Here, we report that the major population of intestinal lamina propria lymphocytes expressing IL-1 receptor 1 (IL-1R1) is the lymphoid tissue inducer (LTi)-like cell, a type of innate lymphoid cell. These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling. LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium. Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota. LTi-like cells require IL-1R1 for production of protective cytokines and confer protection in infectious colitis, and their cell numbers in the colon depend upon having a microbiome.

Show MeSH

Related in: MedlinePlus

Number of colonic IL-1R1+ CD4+ LTi-like cells depends on the gut flora.(A) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF, GF, monocolonized with B. fragilis (BF), or monocolonized with segmented filamentous bacteria (SFB). n = 8−10. (B) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of SPF Swiss-Webster mice treated for five weeks with either vancomycin (vanco), neomycin (neo), or metronidazole (metro). N = 8−10. (C) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF or born GF and co-housed at weaning age with SPF mice (GF→SPF). n = 4−5. *, p<0.05; ***, p<0.001; NS, not significant. For box and whisker plots, line represents median, box represents 25th to 75th percentile range, and whiskers represent range.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3672157&req=5

pone-0065405-g005: Number of colonic IL-1R1+ CD4+ LTi-like cells depends on the gut flora.(A) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF, GF, monocolonized with B. fragilis (BF), or monocolonized with segmented filamentous bacteria (SFB). n = 8−10. (B) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of SPF Swiss-Webster mice treated for five weeks with either vancomycin (vanco), neomycin (neo), or metronidazole (metro). N = 8−10. (C) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF or born GF and co-housed at weaning age with SPF mice (GF→SPF). n = 4−5. *, p<0.05; ***, p<0.001; NS, not significant. For box and whisker plots, line represents median, box represents 25th to 75th percentile range, and whiskers represent range.

Mentions: The effect of the intestinal microbiome on the development and function of the intestinal mucosal immune system is becoming increasingly recognized [49]. Although small-intestinal LTi-like cell numbers are not dependent on the microbiome [26], we sought to examine whether the microbiome affects cLP LTi-like cells. We found that GF mice have a lower absolute number of cLP IL-1R1+ CD4+ LTi-like cells than SPF mice (Fig. 5A), demonstrating that cLP LTi-like cell numbers depend on host-bacterial interactions. To investigate whether these interactions require specific bacteria or merely the presence of any bacteria (perhaps through general microbe-associated molecular patterns such as Toll-like receptors), we analyzed mice monocolonized with B. fragilis or SFB, prototypical Gram-negative and Gram-positive organisms, respectively, that are known to have significant immunomodulatory effects on the intestinal immune system [6], [8], [9]. Neither organism alone increased the number of cLP LTi-like cells above that of GF mice (Fig. 5A), revealing that cLP LTi-like cells depend on specific bacteria other than the two organisms tested.


Role of murine intestinal interleukin-1 receptor 1-expressing lymphoid tissue inducer-like cells in Salmonella infection.

Chen VL, Surana NK, Duan J, Kasper DL - PLoS ONE (2013)

Number of colonic IL-1R1+ CD4+ LTi-like cells depends on the gut flora.(A) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF, GF, monocolonized with B. fragilis (BF), or monocolonized with segmented filamentous bacteria (SFB). n = 8−10. (B) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of SPF Swiss-Webster mice treated for five weeks with either vancomycin (vanco), neomycin (neo), or metronidazole (metro). N = 8−10. (C) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF or born GF and co-housed at weaning age with SPF mice (GF→SPF). n = 4−5. *, p<0.05; ***, p<0.001; NS, not significant. For box and whisker plots, line represents median, box represents 25th to 75th percentile range, and whiskers represent range.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672157&req=5

pone-0065405-g005: Number of colonic IL-1R1+ CD4+ LTi-like cells depends on the gut flora.(A) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF, GF, monocolonized with B. fragilis (BF), or monocolonized with segmented filamentous bacteria (SFB). n = 8−10. (B) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of SPF Swiss-Webster mice treated for five weeks with either vancomycin (vanco), neomycin (neo), or metronidazole (metro). N = 8−10. (C) Number of IL-1R1+ CD4+ LTi-like cells in the cLP of Swiss-Webster mice that were either SPF or born GF and co-housed at weaning age with SPF mice (GF→SPF). n = 4−5. *, p<0.05; ***, p<0.001; NS, not significant. For box and whisker plots, line represents median, box represents 25th to 75th percentile range, and whiskers represent range.
Mentions: The effect of the intestinal microbiome on the development and function of the intestinal mucosal immune system is becoming increasingly recognized [49]. Although small-intestinal LTi-like cell numbers are not dependent on the microbiome [26], we sought to examine whether the microbiome affects cLP LTi-like cells. We found that GF mice have a lower absolute number of cLP IL-1R1+ CD4+ LTi-like cells than SPF mice (Fig. 5A), demonstrating that cLP LTi-like cell numbers depend on host-bacterial interactions. To investigate whether these interactions require specific bacteria or merely the presence of any bacteria (perhaps through general microbe-associated molecular patterns such as Toll-like receptors), we analyzed mice monocolonized with B. fragilis or SFB, prototypical Gram-negative and Gram-positive organisms, respectively, that are known to have significant immunomodulatory effects on the intestinal immune system [6], [8], [9]. Neither organism alone increased the number of cLP LTi-like cells above that of GF mice (Fig. 5A), revealing that cLP LTi-like cells depend on specific bacteria other than the two organisms tested.

Bottom Line: These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling.LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium.Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA.

ABSTRACT
Interleukin (IL)-1 signaling plays a critical role in intestinal immunology. Here, we report that the major population of intestinal lamina propria lymphocytes expressing IL-1 receptor 1 (IL-1R1) is the lymphoid tissue inducer (LTi)-like cell, a type of innate lymphoid cell. These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling. LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium. Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota. LTi-like cells require IL-1R1 for production of protective cytokines and confer protection in infectious colitis, and their cell numbers in the colon depend upon having a microbiome.

Show MeSH
Related in: MedlinePlus