Limits...
Role of murine intestinal interleukin-1 receptor 1-expressing lymphoid tissue inducer-like cells in Salmonella infection.

Chen VL, Surana NK, Duan J, Kasper DL - PLoS ONE (2013)

Bottom Line: These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling.LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium.Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA.

ABSTRACT
Interleukin (IL)-1 signaling plays a critical role in intestinal immunology. Here, we report that the major population of intestinal lamina propria lymphocytes expressing IL-1 receptor 1 (IL-1R1) is the lymphoid tissue inducer (LTi)-like cell, a type of innate lymphoid cell. These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling. LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium. Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota. LTi-like cells require IL-1R1 for production of protective cytokines and confer protection in infectious colitis, and their cell numbers in the colon depend upon having a microbiome.

Show MeSH

Related in: MedlinePlus

Gut-associated IL-1R1+ CD4+ Lin− LTi-like cells are located predominantly in the intestinal lamina propria.(A) Representative FACS scatter plots depicting IL-1R1+ cells as a percentage of total CD4+ or CD8+ cells in spleen and gut-associated compartments of WT C57BL/6J mice. Numbers represent percentage of CD4+ or CD8+ cells expressing IL-1R1. (B and C) Bar graph depicting IL-1R1+ cells as a percentage of total CD4+ (B) or CD8+ (C) cells in spleen and gut-associated compartments of WT C57BL/6J mice. Bar represents median; error bar represents standard error. Filled bars represent anti-IL-1R1 flurophore-conjugated antibodies; empty bars represent isotype control antibodies. Spl, spleen; MLN, mesenteric lymph nodes; SI, small intestine; IEL, intraepithelial lymphocytes; LPL, lamina propria lymphocytes. Data collected from three independent experiments. (D) Gating scheme for IL-1R1+ CD4+ cells. SSC, side scatter. FSC, forward scatter. Number is the percent of CD4+ CD3− lymphocytes that are IL-1R1+ in WT C57BL/6J mice. (E) Scatter plot depicting IL-1R1+ CD4+ cells in Rag1−/− C57BL/6J mice. Number is the percent of CD4+ lymphocytes that are IL-1R1+ in Rag1−/− mice. Gated on CD4+ lymphocytes as in (D). (F) FACS histograms demonstrating expression of c-kit, CD127, RORγt, and Lin in WT C57BL/6J mice. Plots are gated on IL-1R1+ CD4+ cells. Empty, isotype control; filled, antibody. *, p<0.05; **, p<0.01; NS, not significant.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3672157&req=5

pone-0065405-g001: Gut-associated IL-1R1+ CD4+ Lin− LTi-like cells are located predominantly in the intestinal lamina propria.(A) Representative FACS scatter plots depicting IL-1R1+ cells as a percentage of total CD4+ or CD8+ cells in spleen and gut-associated compartments of WT C57BL/6J mice. Numbers represent percentage of CD4+ or CD8+ cells expressing IL-1R1. (B and C) Bar graph depicting IL-1R1+ cells as a percentage of total CD4+ (B) or CD8+ (C) cells in spleen and gut-associated compartments of WT C57BL/6J mice. Bar represents median; error bar represents standard error. Filled bars represent anti-IL-1R1 flurophore-conjugated antibodies; empty bars represent isotype control antibodies. Spl, spleen; MLN, mesenteric lymph nodes; SI, small intestine; IEL, intraepithelial lymphocytes; LPL, lamina propria lymphocytes. Data collected from three independent experiments. (D) Gating scheme for IL-1R1+ CD4+ cells. SSC, side scatter. FSC, forward scatter. Number is the percent of CD4+ CD3− lymphocytes that are IL-1R1+ in WT C57BL/6J mice. (E) Scatter plot depicting IL-1R1+ CD4+ cells in Rag1−/− C57BL/6J mice. Number is the percent of CD4+ lymphocytes that are IL-1R1+ in Rag1−/− mice. Gated on CD4+ lymphocytes as in (D). (F) FACS histograms demonstrating expression of c-kit, CD127, RORγt, and Lin in WT C57BL/6J mice. Plots are gated on IL-1R1+ CD4+ cells. Empty, isotype control; filled, antibody. *, p<0.05; **, p<0.01; NS, not significant.

Mentions: Previous work in our laboratory demonstrated the importance of IL-1R1 signaling in the production of IL-17 by γδ T cells in mucosal and systemic immune compartments [48]. We were interested in extending this work by investigating IL-1R1 expression on T cells in the intestinal immune system. We isolated lymphocytes from the spleen and several gut-associated compartments of wild-type (WT) mice and analyzed them with fluorophore-conjugated antibodies against IL-1R1 in conjunction with antibodies against either CD4 or CD8. Flow cytometric analysis demonstrated that there are populations of CD4+ cells in the small-intestinal and colonic lamina propria (cLP) that express IL-1R1 (Fig. 1A and 1B). In contrast, virtually no CD8+ cells express IL-1R1 in the investigated compartments (Fig. 1A and 1C).


Role of murine intestinal interleukin-1 receptor 1-expressing lymphoid tissue inducer-like cells in Salmonella infection.

Chen VL, Surana NK, Duan J, Kasper DL - PLoS ONE (2013)

Gut-associated IL-1R1+ CD4+ Lin− LTi-like cells are located predominantly in the intestinal lamina propria.(A) Representative FACS scatter plots depicting IL-1R1+ cells as a percentage of total CD4+ or CD8+ cells in spleen and gut-associated compartments of WT C57BL/6J mice. Numbers represent percentage of CD4+ or CD8+ cells expressing IL-1R1. (B and C) Bar graph depicting IL-1R1+ cells as a percentage of total CD4+ (B) or CD8+ (C) cells in spleen and gut-associated compartments of WT C57BL/6J mice. Bar represents median; error bar represents standard error. Filled bars represent anti-IL-1R1 flurophore-conjugated antibodies; empty bars represent isotype control antibodies. Spl, spleen; MLN, mesenteric lymph nodes; SI, small intestine; IEL, intraepithelial lymphocytes; LPL, lamina propria lymphocytes. Data collected from three independent experiments. (D) Gating scheme for IL-1R1+ CD4+ cells. SSC, side scatter. FSC, forward scatter. Number is the percent of CD4+ CD3− lymphocytes that are IL-1R1+ in WT C57BL/6J mice. (E) Scatter plot depicting IL-1R1+ CD4+ cells in Rag1−/− C57BL/6J mice. Number is the percent of CD4+ lymphocytes that are IL-1R1+ in Rag1−/− mice. Gated on CD4+ lymphocytes as in (D). (F) FACS histograms demonstrating expression of c-kit, CD127, RORγt, and Lin in WT C57BL/6J mice. Plots are gated on IL-1R1+ CD4+ cells. Empty, isotype control; filled, antibody. *, p<0.05; **, p<0.01; NS, not significant.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672157&req=5

pone-0065405-g001: Gut-associated IL-1R1+ CD4+ Lin− LTi-like cells are located predominantly in the intestinal lamina propria.(A) Representative FACS scatter plots depicting IL-1R1+ cells as a percentage of total CD4+ or CD8+ cells in spleen and gut-associated compartments of WT C57BL/6J mice. Numbers represent percentage of CD4+ or CD8+ cells expressing IL-1R1. (B and C) Bar graph depicting IL-1R1+ cells as a percentage of total CD4+ (B) or CD8+ (C) cells in spleen and gut-associated compartments of WT C57BL/6J mice. Bar represents median; error bar represents standard error. Filled bars represent anti-IL-1R1 flurophore-conjugated antibodies; empty bars represent isotype control antibodies. Spl, spleen; MLN, mesenteric lymph nodes; SI, small intestine; IEL, intraepithelial lymphocytes; LPL, lamina propria lymphocytes. Data collected from three independent experiments. (D) Gating scheme for IL-1R1+ CD4+ cells. SSC, side scatter. FSC, forward scatter. Number is the percent of CD4+ CD3− lymphocytes that are IL-1R1+ in WT C57BL/6J mice. (E) Scatter plot depicting IL-1R1+ CD4+ cells in Rag1−/− C57BL/6J mice. Number is the percent of CD4+ lymphocytes that are IL-1R1+ in Rag1−/− mice. Gated on CD4+ lymphocytes as in (D). (F) FACS histograms demonstrating expression of c-kit, CD127, RORγt, and Lin in WT C57BL/6J mice. Plots are gated on IL-1R1+ CD4+ cells. Empty, isotype control; filled, antibody. *, p<0.05; **, p<0.01; NS, not significant.
Mentions: Previous work in our laboratory demonstrated the importance of IL-1R1 signaling in the production of IL-17 by γδ T cells in mucosal and systemic immune compartments [48]. We were interested in extending this work by investigating IL-1R1 expression on T cells in the intestinal immune system. We isolated lymphocytes from the spleen and several gut-associated compartments of wild-type (WT) mice and analyzed them with fluorophore-conjugated antibodies against IL-1R1 in conjunction with antibodies against either CD4 or CD8. Flow cytometric analysis demonstrated that there are populations of CD4+ cells in the small-intestinal and colonic lamina propria (cLP) that express IL-1R1 (Fig. 1A and 1B). In contrast, virtually no CD8+ cells express IL-1R1 in the investigated compartments (Fig. 1A and 1C).

Bottom Line: These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling.LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium.Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA.

ABSTRACT
Interleukin (IL)-1 signaling plays a critical role in intestinal immunology. Here, we report that the major population of intestinal lamina propria lymphocytes expressing IL-1 receptor 1 (IL-1R1) is the lymphoid tissue inducer (LTi)-like cell, a type of innate lymphoid cell. These cells are significant producers of IL-22, and this IL-22 production depends on IL-1R1 signaling. LTi-like cells are required for defense against Salmonella enterica serovar Typhimurium. Moreover, colonic LTi-like cell numbers depend on the presence of the intestinal microbiota. LTi-like cells require IL-1R1 for production of protective cytokines and confer protection in infectious colitis, and their cell numbers in the colon depend upon having a microbiome.

Show MeSH
Related in: MedlinePlus