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Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

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RT-PCR analysis of Hsp70 gene expression in gonad samples of the swamp eel.Most of the Hsp70 family genes were expressed equally in different types of gonads except Hspa4L, Hspa5, Hspa8b2, Hspa9 and Hspa12a. Hspa8b2 was expressed differentially among testis, ovotestis and ovary. Hprt was used as an internal control. Length sizes of amplified products were shown on the right.
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pone-0065269-g005: RT-PCR analysis of Hsp70 gene expression in gonad samples of the swamp eel.Most of the Hsp70 family genes were expressed equally in different types of gonads except Hspa4L, Hspa5, Hspa8b2, Hspa9 and Hspa12a. Hspa8b2 was expressed differentially among testis, ovotestis and ovary. Hprt was used as an internal control. Length sizes of amplified products were shown on the right.

Mentions: We further analyzed expression pattern of Hsp70 family genes in three types of gonads in the swamp eel by RT-PCR. Most of the Hsp70 genes were expressed equally in different types of gonads except Hspa4L, Hspa5, Hspa8b2 and Hspa9. Hspa9 was expressed faintly in gonads. Only Hspa8b2 and Hspa5 were upregulated from ovary, ovotestis to testis (Figure 5). Hspa8b2 gene has Hspa1–2_6–8-like_NBD conserved domain, which is similar to the protein identified by the MS analysis. Together with phylogenetic analysis, these results indicated that the testis-enriched gene may be Hspa8b2 in the swamp eel, instead of Hspa2.


Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

RT-PCR analysis of Hsp70 gene expression in gonad samples of the swamp eel.Most of the Hsp70 family genes were expressed equally in different types of gonads except Hspa4L, Hspa5, Hspa8b2, Hspa9 and Hspa12a. Hspa8b2 was expressed differentially among testis, ovotestis and ovary. Hprt was used as an internal control. Length sizes of amplified products were shown on the right.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3672149&req=5

pone-0065269-g005: RT-PCR analysis of Hsp70 gene expression in gonad samples of the swamp eel.Most of the Hsp70 family genes were expressed equally in different types of gonads except Hspa4L, Hspa5, Hspa8b2, Hspa9 and Hspa12a. Hspa8b2 was expressed differentially among testis, ovotestis and ovary. Hprt was used as an internal control. Length sizes of amplified products were shown on the right.
Mentions: We further analyzed expression pattern of Hsp70 family genes in three types of gonads in the swamp eel by RT-PCR. Most of the Hsp70 genes were expressed equally in different types of gonads except Hspa4L, Hspa5, Hspa8b2 and Hspa9. Hspa9 was expressed faintly in gonads. Only Hspa8b2 and Hspa5 were upregulated from ovary, ovotestis to testis (Figure 5). Hspa8b2 gene has Hspa1–2_6–8-like_NBD conserved domain, which is similar to the protein identified by the MS analysis. Together with phylogenetic analysis, these results indicated that the testis-enriched gene may be Hspa8b2 in the swamp eel, instead of Hspa2.

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

Show MeSH
Related in: MedlinePlus