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Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

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Related in: MedlinePlus

Copy number variation analysis of Hsp70 family genes from various vertebrates.Hspa5 and Hspa9 had one copy in these species, while copy numbers of the other genes ranged from 0 to 5. Hspa4 and Hspa8 had duplicated in fishes, while Hspa2 and Hspa6 may be lost in fishes (previously named Hspa2 in medaka would be Hspa1). Squares with different colors represent different genes and copies, while white squares denote the genes, which have not been detected in these species.
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pone-0065269-g004: Copy number variation analysis of Hsp70 family genes from various vertebrates.Hspa5 and Hspa9 had one copy in these species, while copy numbers of the other genes ranged from 0 to 5. Hspa4 and Hspa8 had duplicated in fishes, while Hspa2 and Hspa6 may be lost in fishes (previously named Hspa2 in medaka would be Hspa1). Squares with different colors represent different genes and copies, while white squares denote the genes, which have not been detected in these species.

Mentions: As Hsp70 family members have many synonyms, we aligned all Hsp70 members of vertebrates and analyzed their clusters using the Maximum Likelihood method. A phylogenetic tree was finally constructed (Figure 3). Each Hsp70 gene in the swamp eel could be grouped into a cluster of Hsp70 family. The evolutionary analysis of these Hsp70 family genes in vertebrates showed that more copies of Hsp70 members were observed in fishes than mammals (Figure 4), especially 3–5 copies of Hspa4 in the fish species. Because of a high level of homology between Hspa8 and Hspa2, we further compared their sequence homology to determine the branching of the Hspa8 and Hspa2 genes. The protein sequence alignments of the swamp eel Hspa8 (–8a1/−8a2 and –8b1/−8b2) with the mammalian Hspa8 and Hspa2 indicated that the swamp eel Hspa8b2 was more similar to Hspa8 (positive and identity of Hspa8b2 with Hspa8 of human and mouse: 100% and 86.7%; Hspa8b2 with Hspa2 of human and rat: 98.9% and 82.5%, respectively) (Figure S1 a, b, c). Thus four copies of Hspa8 genes (named as Hspa8a1/−a2 and Hspa8b1/−b2) in the swamp eel were further confirmed. Hspa2 and Hspa6 may be lost in the teleost fish species (Figure 4), and previously named Hspa2 in medaka may be Hspa1b (Figure 3).


Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

Copy number variation analysis of Hsp70 family genes from various vertebrates.Hspa5 and Hspa9 had one copy in these species, while copy numbers of the other genes ranged from 0 to 5. Hspa4 and Hspa8 had duplicated in fishes, while Hspa2 and Hspa6 may be lost in fishes (previously named Hspa2 in medaka would be Hspa1). Squares with different colors represent different genes and copies, while white squares denote the genes, which have not been detected in these species.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672149&req=5

pone-0065269-g004: Copy number variation analysis of Hsp70 family genes from various vertebrates.Hspa5 and Hspa9 had one copy in these species, while copy numbers of the other genes ranged from 0 to 5. Hspa4 and Hspa8 had duplicated in fishes, while Hspa2 and Hspa6 may be lost in fishes (previously named Hspa2 in medaka would be Hspa1). Squares with different colors represent different genes and copies, while white squares denote the genes, which have not been detected in these species.
Mentions: As Hsp70 family members have many synonyms, we aligned all Hsp70 members of vertebrates and analyzed their clusters using the Maximum Likelihood method. A phylogenetic tree was finally constructed (Figure 3). Each Hsp70 gene in the swamp eel could be grouped into a cluster of Hsp70 family. The evolutionary analysis of these Hsp70 family genes in vertebrates showed that more copies of Hsp70 members were observed in fishes than mammals (Figure 4), especially 3–5 copies of Hspa4 in the fish species. Because of a high level of homology between Hspa8 and Hspa2, we further compared their sequence homology to determine the branching of the Hspa8 and Hspa2 genes. The protein sequence alignments of the swamp eel Hspa8 (–8a1/−8a2 and –8b1/−8b2) with the mammalian Hspa8 and Hspa2 indicated that the swamp eel Hspa8b2 was more similar to Hspa8 (positive and identity of Hspa8b2 with Hspa8 of human and mouse: 100% and 86.7%; Hspa8b2 with Hspa2 of human and rat: 98.9% and 82.5%, respectively) (Figure S1 a, b, c). Thus four copies of Hspa8 genes (named as Hspa8a1/−a2 and Hspa8b1/−b2) in the swamp eel were further confirmed. Hspa2 and Hspa6 may be lost in the teleost fish species (Figure 4), and previously named Hspa2 in medaka may be Hspa1b (Figure 3).

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

Show MeSH
Related in: MedlinePlus