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Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

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Related in: MedlinePlus

Phylogenetic tree of Hsp70 family in vertebrates.The phylogenetic tree was constructed using the Maximum Likelihood method based on the amino acids of Hsp70 members from human, mouse, rat, camel, cattle, platypus, chicken, frog, zebrafish, pufferfish, fugu, stickleback, medaka, tilapia and the swamp eel. The protein sequences were from NCBI and Ensembl database (protein ID in Table S1). 14 proteins of the swamp eel had been grouped into 7 cluster of Hsp70 family (red arrows). The numbers at the nodes indicate bootstrap values.
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pone-0065269-g003: Phylogenetic tree of Hsp70 family in vertebrates.The phylogenetic tree was constructed using the Maximum Likelihood method based on the amino acids of Hsp70 members from human, mouse, rat, camel, cattle, platypus, chicken, frog, zebrafish, pufferfish, fugu, stickleback, medaka, tilapia and the swamp eel. The protein sequences were from NCBI and Ensembl database (protein ID in Table S1). 14 proteins of the swamp eel had been grouped into 7 cluster of Hsp70 family (red arrows). The numbers at the nodes indicate bootstrap values.

Mentions: As Hsp70 family members have many synonyms, we aligned all Hsp70 members of vertebrates and analyzed their clusters using the Maximum Likelihood method. A phylogenetic tree was finally constructed (Figure 3). Each Hsp70 gene in the swamp eel could be grouped into a cluster of Hsp70 family. The evolutionary analysis of these Hsp70 family genes in vertebrates showed that more copies of Hsp70 members were observed in fishes than mammals (Figure 4), especially 3–5 copies of Hspa4 in the fish species. Because of a high level of homology between Hspa8 and Hspa2, we further compared their sequence homology to determine the branching of the Hspa8 and Hspa2 genes. The protein sequence alignments of the swamp eel Hspa8 (–8a1/−8a2 and –8b1/−8b2) with the mammalian Hspa8 and Hspa2 indicated that the swamp eel Hspa8b2 was more similar to Hspa8 (positive and identity of Hspa8b2 with Hspa8 of human and mouse: 100% and 86.7%; Hspa8b2 with Hspa2 of human and rat: 98.9% and 82.5%, respectively) (Figure S1 a, b, c). Thus four copies of Hspa8 genes (named as Hspa8a1/−a2 and Hspa8b1/−b2) in the swamp eel were further confirmed. Hspa2 and Hspa6 may be lost in the teleost fish species (Figure 4), and previously named Hspa2 in medaka may be Hspa1b (Figure 3).


Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

Phylogenetic tree of Hsp70 family in vertebrates.The phylogenetic tree was constructed using the Maximum Likelihood method based on the amino acids of Hsp70 members from human, mouse, rat, camel, cattle, platypus, chicken, frog, zebrafish, pufferfish, fugu, stickleback, medaka, tilapia and the swamp eel. The protein sequences were from NCBI and Ensembl database (protein ID in Table S1). 14 proteins of the swamp eel had been grouped into 7 cluster of Hsp70 family (red arrows). The numbers at the nodes indicate bootstrap values.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672149&req=5

pone-0065269-g003: Phylogenetic tree of Hsp70 family in vertebrates.The phylogenetic tree was constructed using the Maximum Likelihood method based on the amino acids of Hsp70 members from human, mouse, rat, camel, cattle, platypus, chicken, frog, zebrafish, pufferfish, fugu, stickleback, medaka, tilapia and the swamp eel. The protein sequences were from NCBI and Ensembl database (protein ID in Table S1). 14 proteins of the swamp eel had been grouped into 7 cluster of Hsp70 family (red arrows). The numbers at the nodes indicate bootstrap values.
Mentions: As Hsp70 family members have many synonyms, we aligned all Hsp70 members of vertebrates and analyzed their clusters using the Maximum Likelihood method. A phylogenetic tree was finally constructed (Figure 3). Each Hsp70 gene in the swamp eel could be grouped into a cluster of Hsp70 family. The evolutionary analysis of these Hsp70 family genes in vertebrates showed that more copies of Hsp70 members were observed in fishes than mammals (Figure 4), especially 3–5 copies of Hspa4 in the fish species. Because of a high level of homology between Hspa8 and Hspa2, we further compared their sequence homology to determine the branching of the Hspa8 and Hspa2 genes. The protein sequence alignments of the swamp eel Hspa8 (–8a1/−8a2 and –8b1/−8b2) with the mammalian Hspa8 and Hspa2 indicated that the swamp eel Hspa8b2 was more similar to Hspa8 (positive and identity of Hspa8b2 with Hspa8 of human and mouse: 100% and 86.7%; Hspa8b2 with Hspa2 of human and rat: 98.9% and 82.5%, respectively) (Figure S1 a, b, c). Thus four copies of Hspa8 genes (named as Hspa8a1/−a2 and Hspa8b1/−b2) in the swamp eel were further confirmed. Hspa2 and Hspa6 may be lost in the teleost fish species (Figure 4), and previously named Hspa2 in medaka may be Hspa1b (Figure 3).

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

Show MeSH
Related in: MedlinePlus