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Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

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Related in: MedlinePlus

Conserved domain analysis of Hsp70 family members in swamp eel.All the 14 proteins had NBD_sugar-kinase_HSP70_actin superfamily domain (green bars), and some genes had their own conserved domain (yellow bars). Hspa1a/b and Hspa8a1/a2/b1/b2 had a common domain (HSPA1–2_6–8-like_NBD). The numbers on the bars denote amino acid positions. GenBank accession numbers were shown in Table S1.
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pone-0065269-g002: Conserved domain analysis of Hsp70 family members in swamp eel.All the 14 proteins had NBD_sugar-kinase_HSP70_actin superfamily domain (green bars), and some genes had their own conserved domain (yellow bars). Hspa1a/b and Hspa8a1/a2/b1/b2 had a common domain (HSPA1–2_6–8-like_NBD). The numbers on the bars denote amino acid positions. GenBank accession numbers were shown in Table S1.

Mentions: To further characterize the testis-enriched gene, we cloned 14 members of Hsp70 family from the swamp eel (Table S1 and Figure 2). Conserved domain analysis showed that Hspa1a/b and Hspa8a/b had the same Hspa1–2_6–8-like_NBD domain (Figure 2). Further sequence analysis showed that there were four copies of Hspa8 (–8a1/−8a2 and –8b1/−8b2), three copies of Hspa4 (–4a/−4b and –4L), two copies of Hspa1 (–1a/−1b) and Hspa12 (–12a/−12b), one copy of Hspa5, Hspa9 and Hspa14 in the swamp eel.


Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

He Y, Luo M, Yi M, Sheng Y, Cheng Y, Zhou R, Cheng H - PLoS ONE (2013)

Conserved domain analysis of Hsp70 family members in swamp eel.All the 14 proteins had NBD_sugar-kinase_HSP70_actin superfamily domain (green bars), and some genes had their own conserved domain (yellow bars). Hspa1a/b and Hspa8a1/a2/b1/b2 had a common domain (HSPA1–2_6–8-like_NBD). The numbers on the bars denote amino acid positions. GenBank accession numbers were shown in Table S1.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672149&req=5

pone-0065269-g002: Conserved domain analysis of Hsp70 family members in swamp eel.All the 14 proteins had NBD_sugar-kinase_HSP70_actin superfamily domain (green bars), and some genes had their own conserved domain (yellow bars). Hspa1a/b and Hspa8a1/a2/b1/b2 had a common domain (HSPA1–2_6–8-like_NBD). The numbers on the bars denote amino acid positions. GenBank accession numbers were shown in Table S1.
Mentions: To further characterize the testis-enriched gene, we cloned 14 members of Hsp70 family from the swamp eel (Table S1 and Figure 2). Conserved domain analysis showed that Hspa1a/b and Hspa8a/b had the same Hspa1–2_6–8-like_NBD domain (Figure 2). Further sequence analysis showed that there were four copies of Hspa8 (–8a1/−8a2 and –8b1/−8b2), three copies of Hspa4 (–4a/−4b and –4L), two copies of Hspa1 (–1a/−1b) and Hspa12 (–12a/−12b), one copy of Hspa5, Hspa9 and Hspa14 in the swamp eel.

Bottom Line: There are fourteen gene members in the Hsp70 family in the swamp eel genome.Hsp70 family, particularly Hspa8, has expanded in the species.One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, PR China.

ABSTRACT

Background: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood.

Methods and findings: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).

Conclusion: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.

Show MeSH
Related in: MedlinePlus