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A cotton annexin protein AnxGb6 regulates fiber elongation through its interaction with actin 1.

Huang Y, Wang J, Zhang L, Zuo K - PLoS ONE (2013)

Bottom Line: Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific.Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate.Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, SJTU-Cornell Institute of Sustainable Agriculture and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains to be presented. Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific. Yeast two hybridization and BiFC analysis revealed that AnxGb6 homodimer interacted with a cotton fiber specific actin GbAct1. Ectopic-expressed AnxGb6 in Arabidopsis enhanced its root elongation without increasing the root cell number. Ectopic AnxGb6 expression resulted in more F-actin accumulation in the basal part of the root cell elongation zone. Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate. Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

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Cotton annexin protein sub-cellular localization analysis.A: AnxGb1-GFP, B: AnxGb4-GFP, C: AnxGb6-GFP and D: Control plants, expressing 35S::GFP. Right is the corresponding bright-field. Left is the corresponding black-field. Scale bar: 25 µm.
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pone-0066160-g004: Cotton annexin protein sub-cellular localization analysis.A: AnxGb1-GFP, B: AnxGb4-GFP, C: AnxGb6-GFP and D: Control plants, expressing 35S::GFP. Right is the corresponding bright-field. Left is the corresponding black-field. Scale bar: 25 µm.

Mentions: In order to investigate the sub-cellular location of G. barbadense annexins, three genes AnxGb1, 4, 6 from different cotton annexin groups were chosen to generate GFP fusion protein constructs (pBIB-35S::AnxGb1, 4, 6-GFP). The constructs were introduced into Agrobacterium EHA105 and infiltrated into tobacco leaf cells. Confocal microscopy showed that the three annexins, AnxGb1, 4, 6 had the same sub-cellular location, and had a high expression in the plasma membrane and nucleolus (Figure 4).


A cotton annexin protein AnxGb6 regulates fiber elongation through its interaction with actin 1.

Huang Y, Wang J, Zhang L, Zuo K - PLoS ONE (2013)

Cotton annexin protein sub-cellular localization analysis.A: AnxGb1-GFP, B: AnxGb4-GFP, C: AnxGb6-GFP and D: Control plants, expressing 35S::GFP. Right is the corresponding bright-field. Left is the corresponding black-field. Scale bar: 25 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672135&req=5

pone-0066160-g004: Cotton annexin protein sub-cellular localization analysis.A: AnxGb1-GFP, B: AnxGb4-GFP, C: AnxGb6-GFP and D: Control plants, expressing 35S::GFP. Right is the corresponding bright-field. Left is the corresponding black-field. Scale bar: 25 µm.
Mentions: In order to investigate the sub-cellular location of G. barbadense annexins, three genes AnxGb1, 4, 6 from different cotton annexin groups were chosen to generate GFP fusion protein constructs (pBIB-35S::AnxGb1, 4, 6-GFP). The constructs were introduced into Agrobacterium EHA105 and infiltrated into tobacco leaf cells. Confocal microscopy showed that the three annexins, AnxGb1, 4, 6 had the same sub-cellular location, and had a high expression in the plasma membrane and nucleolus (Figure 4).

Bottom Line: Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific.Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate.Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, SJTU-Cornell Institute of Sustainable Agriculture and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains to be presented. Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific. Yeast two hybridization and BiFC analysis revealed that AnxGb6 homodimer interacted with a cotton fiber specific actin GbAct1. Ectopic-expressed AnxGb6 in Arabidopsis enhanced its root elongation without increasing the root cell number. Ectopic AnxGb6 expression resulted in more F-actin accumulation in the basal part of the root cell elongation zone. Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate. Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

Show MeSH
Related in: MedlinePlus