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A cotton annexin protein AnxGb6 regulates fiber elongation through its interaction with actin 1.

Huang Y, Wang J, Zhang L, Zuo K - PLoS ONE (2013)

Bottom Line: Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific.Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate.Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, SJTU-Cornell Institute of Sustainable Agriculture and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains to be presented. Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific. Yeast two hybridization and BiFC analysis revealed that AnxGb6 homodimer interacted with a cotton fiber specific actin GbAct1. Ectopic-expressed AnxGb6 in Arabidopsis enhanced its root elongation without increasing the root cell number. Ectopic AnxGb6 expression resulted in more F-actin accumulation in the basal part of the root cell elongation zone. Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate. Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

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Phylogenetic analysis of G. barbadense annexins.Nineteen of the complete amino acid sequences of annexin proteins were used to generate the Neighbor–Joining tree, and the numbers next to each node give bootstrap values from 1000 replicates.(AnxGh1: AAR13288.1; AnxGh2: AAB67994.1; AnxGhF: AAC33305.1; AnxGhFx: FJ415173; AnxAt1: NP_174810.1; AnxAt2: NP_201307.1; AnxAt3: NP_181410.1; AnxAt4: NP_181409.1; AnxAt5: NP_564920.1; AnxAt6: NP_196584.1; AnxAt7: NP_196585.1; AnxAt8: NP_568271.2; AnxZm2: NP_001105475.1; AnxZm4: NP_001147343.1; AnxZmF: ACF82214.1; AnxZm33: NP_001105728.1; AnxOs1: NP_001061839.1; AnxOs2: NP_001048149.1; AnxOs33: NP_001057176.1)
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pone-0066160-g002: Phylogenetic analysis of G. barbadense annexins.Nineteen of the complete amino acid sequences of annexin proteins were used to generate the Neighbor–Joining tree, and the numbers next to each node give bootstrap values from 1000 replicates.(AnxGh1: AAR13288.1; AnxGh2: AAB67994.1; AnxGhF: AAC33305.1; AnxGhFx: FJ415173; AnxAt1: NP_174810.1; AnxAt2: NP_201307.1; AnxAt3: NP_181410.1; AnxAt4: NP_181409.1; AnxAt5: NP_564920.1; AnxAt6: NP_196584.1; AnxAt7: NP_196585.1; AnxAt8: NP_568271.2; AnxZm2: NP_001105475.1; AnxZm4: NP_001147343.1; AnxZmF: ACF82214.1; AnxZm33: NP_001105728.1; AnxOs1: NP_001061839.1; AnxOs2: NP_001048149.1; AnxOs33: NP_001057176.1)

Mentions: In order to look more closely at the relationships between G.barbadense annexins and the other members of the plant annexin protein family, the multiple alignments of full-length protein sequences were used to construct a Neighbor-Joining phylogenetic tree. The phylogenetic tree includes 6 G. barbadense annexin proteins, AnxGh1, AnxGh2, AnxGhF and AnxGhFx in cotton; AnxAt1-8 in Arabidopsis; AnxZm2, AnxZm4, AnxZmF and AnxZm33 in Maize; AnxOs1, AnxOs2 and AnxOs33 in Rice (Figure 2). Phylogenetic analysis revealed that 6 G. barbadense annexins genes were classified into 3 groups, which was consistent with the alignment result. The deduced amino acid sequences for AnxGb5 and 6, have predicted actin-binding sites, while the other G. barbadense annexins do not, indicating that they may have a distinct function in cotton fiber development. Therefore, we used expression pattern analysis and sub-cellular localization analysis to verify these inferences.


A cotton annexin protein AnxGb6 regulates fiber elongation through its interaction with actin 1.

Huang Y, Wang J, Zhang L, Zuo K - PLoS ONE (2013)

Phylogenetic analysis of G. barbadense annexins.Nineteen of the complete amino acid sequences of annexin proteins were used to generate the Neighbor–Joining tree, and the numbers next to each node give bootstrap values from 1000 replicates.(AnxGh1: AAR13288.1; AnxGh2: AAB67994.1; AnxGhF: AAC33305.1; AnxGhFx: FJ415173; AnxAt1: NP_174810.1; AnxAt2: NP_201307.1; AnxAt3: NP_181410.1; AnxAt4: NP_181409.1; AnxAt5: NP_564920.1; AnxAt6: NP_196584.1; AnxAt7: NP_196585.1; AnxAt8: NP_568271.2; AnxZm2: NP_001105475.1; AnxZm4: NP_001147343.1; AnxZmF: ACF82214.1; AnxZm33: NP_001105728.1; AnxOs1: NP_001061839.1; AnxOs2: NP_001048149.1; AnxOs33: NP_001057176.1)
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3672135&req=5

pone-0066160-g002: Phylogenetic analysis of G. barbadense annexins.Nineteen of the complete amino acid sequences of annexin proteins were used to generate the Neighbor–Joining tree, and the numbers next to each node give bootstrap values from 1000 replicates.(AnxGh1: AAR13288.1; AnxGh2: AAB67994.1; AnxGhF: AAC33305.1; AnxGhFx: FJ415173; AnxAt1: NP_174810.1; AnxAt2: NP_201307.1; AnxAt3: NP_181410.1; AnxAt4: NP_181409.1; AnxAt5: NP_564920.1; AnxAt6: NP_196584.1; AnxAt7: NP_196585.1; AnxAt8: NP_568271.2; AnxZm2: NP_001105475.1; AnxZm4: NP_001147343.1; AnxZmF: ACF82214.1; AnxZm33: NP_001105728.1; AnxOs1: NP_001061839.1; AnxOs2: NP_001048149.1; AnxOs33: NP_001057176.1)
Mentions: In order to look more closely at the relationships between G.barbadense annexins and the other members of the plant annexin protein family, the multiple alignments of full-length protein sequences were used to construct a Neighbor-Joining phylogenetic tree. The phylogenetic tree includes 6 G. barbadense annexin proteins, AnxGh1, AnxGh2, AnxGhF and AnxGhFx in cotton; AnxAt1-8 in Arabidopsis; AnxZm2, AnxZm4, AnxZmF and AnxZm33 in Maize; AnxOs1, AnxOs2 and AnxOs33 in Rice (Figure 2). Phylogenetic analysis revealed that 6 G. barbadense annexins genes were classified into 3 groups, which was consistent with the alignment result. The deduced amino acid sequences for AnxGb5 and 6, have predicted actin-binding sites, while the other G. barbadense annexins do not, indicating that they may have a distinct function in cotton fiber development. Therefore, we used expression pattern analysis and sub-cellular localization analysis to verify these inferences.

Bottom Line: Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific.Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate.Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, SJTU-Cornell Institute of Sustainable Agriculture and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains to be presented. Here we cloned six Annexin genes (AnxGb) abundantly expressed in fiber from sea-island cotton (G. barbadense). qRT-PCR results indicated that all six G. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of AnxGb6 was cotton fiber-specific. Yeast two hybridization and BiFC analysis revealed that AnxGb6 homodimer interacted with a cotton fiber specific actin GbAct1. Ectopic-expressed AnxGb6 in Arabidopsis enhanced its root elongation without increasing the root cell number. Ectopic AnxGb6 expression resulted in more F-actin accumulation in the basal part of the root cell elongation zone. Analysis of AnxGb6 expression in three cotton genotypes with different fiber length confirmed that AnxGb6 expression was correlated to cotton fiber length, especially fiber elongation rate. Our results demonstrated that AnxGb6 was important for fiber elongation by potentially providing a domain for F-actin organization.

Show MeSH
Related in: MedlinePlus