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Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.).

Liu C, Ma N, Wang PY, Fu N, Shen HL - PLoS ONE (2013)

Bottom Line: Among these unigenes, 27,191 were identified as putative homologs of annotated sequences in the public protein databases, 4,326 and 7,061 unigenes were found to be highly abundant in lines 121A and 121C, respectively.Many of the differentially expressed unigenes represent a set of potential candidate genes associated with the formation or abortion of pollen.The results shed the lights on the occurrence and recovery of the disturbances in nuclear-mitochondrial interaction and provide clues for further investigations.

View Article: PubMed Central - PubMed

Affiliation: China Agricultural University, Beijing, China.

ABSTRACT

Background: The use of cytoplasmic male sterility (CMS) in F1 hybrid seed production of chili pepper is increasingly popular. However, the molecular mechanisms of cytoplasmic male sterility and fertility restoration remain poorly understood due to limited transcriptomic and genomic data. Therefore, we analyzed the difference between a CMS line 121A and its near-isogenic restorer line 121C in transcriptome level using next generation sequencing technology (NGS), aiming to find out critical genes and pathways associated with the male sterility.

Results: We generated approximately 53 million sequencing reads and assembled de novo, yielding 85,144 high quality unigenes with an average length of 643 bp. Among these unigenes, 27,191 were identified as putative homologs of annotated sequences in the public protein databases, 4,326 and 7,061 unigenes were found to be highly abundant in lines 121A and 121C, respectively. Many of the differentially expressed unigenes represent a set of potential candidate genes associated with the formation or abortion of pollen.

Conclusions: Our study profiled anther transcriptomes of a chili pepper CMS line and its restorer line. The results shed the lights on the occurrence and recovery of the disturbances in nuclear-mitochondrial interaction and provide clues for further investigations.

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Related in: MedlinePlus

Examples of sampled flower buds.Phase 1–Phase 5. Representative flower buds collected to produce the samples. Phase 1: Buds were small; sepals wrapped corolla. Phase 2: Buds were a little larger; sepals opened slightly on the top end; the length of sepals was slightly greater than that of petals. Phase 3: Buds swelled obviously; sepals splayed; the length of petals is about the same as or slightly larger than that of sepals. Phase 4: Buds swelled sufficiently; petals overtopped sepals distinctly; sepals attained their sizes. Phase 5: Petals attained their sizes; buds would blossom soon. The upper row was 121A.
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pone-0065209-g008: Examples of sampled flower buds.Phase 1–Phase 5. Representative flower buds collected to produce the samples. Phase 1: Buds were small; sepals wrapped corolla. Phase 2: Buds were a little larger; sepals opened slightly on the top end; the length of sepals was slightly greater than that of petals. Phase 3: Buds swelled obviously; sepals splayed; the length of petals is about the same as or slightly larger than that of sepals. Phase 4: Buds swelled sufficiently; petals overtopped sepals distinctly; sepals attained their sizes. Phase 5: Petals attained their sizes; buds would blossom soon. The upper row was 121A.

Mentions: Flower buds were collected from each of the 20 individuals of 121A and that of 121C, respectively. And collected flower buds were divided into five phases according to the relevance between development phases of microspore and morphological characteristics of floral organs (see Figure 8 for details) [84]. Anthers of the five phases were taken out and frozen in liquid nitrogen. All samples were stored at −80°C until RNA extraction.


Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.).

Liu C, Ma N, Wang PY, Fu N, Shen HL - PLoS ONE (2013)

Examples of sampled flower buds.Phase 1–Phase 5. Representative flower buds collected to produce the samples. Phase 1: Buds were small; sepals wrapped corolla. Phase 2: Buds were a little larger; sepals opened slightly on the top end; the length of sepals was slightly greater than that of petals. Phase 3: Buds swelled obviously; sepals splayed; the length of petals is about the same as or slightly larger than that of sepals. Phase 4: Buds swelled sufficiently; petals overtopped sepals distinctly; sepals attained their sizes. Phase 5: Petals attained their sizes; buds would blossom soon. The upper row was 121A.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3672106&req=5

pone-0065209-g008: Examples of sampled flower buds.Phase 1–Phase 5. Representative flower buds collected to produce the samples. Phase 1: Buds were small; sepals wrapped corolla. Phase 2: Buds were a little larger; sepals opened slightly on the top end; the length of sepals was slightly greater than that of petals. Phase 3: Buds swelled obviously; sepals splayed; the length of petals is about the same as or slightly larger than that of sepals. Phase 4: Buds swelled sufficiently; petals overtopped sepals distinctly; sepals attained their sizes. Phase 5: Petals attained their sizes; buds would blossom soon. The upper row was 121A.
Mentions: Flower buds were collected from each of the 20 individuals of 121A and that of 121C, respectively. And collected flower buds were divided into five phases according to the relevance between development phases of microspore and morphological characteristics of floral organs (see Figure 8 for details) [84]. Anthers of the five phases were taken out and frozen in liquid nitrogen. All samples were stored at −80°C until RNA extraction.

Bottom Line: Among these unigenes, 27,191 were identified as putative homologs of annotated sequences in the public protein databases, 4,326 and 7,061 unigenes were found to be highly abundant in lines 121A and 121C, respectively.Many of the differentially expressed unigenes represent a set of potential candidate genes associated with the formation or abortion of pollen.The results shed the lights on the occurrence and recovery of the disturbances in nuclear-mitochondrial interaction and provide clues for further investigations.

View Article: PubMed Central - PubMed

Affiliation: China Agricultural University, Beijing, China.

ABSTRACT

Background: The use of cytoplasmic male sterility (CMS) in F1 hybrid seed production of chili pepper is increasingly popular. However, the molecular mechanisms of cytoplasmic male sterility and fertility restoration remain poorly understood due to limited transcriptomic and genomic data. Therefore, we analyzed the difference between a CMS line 121A and its near-isogenic restorer line 121C in transcriptome level using next generation sequencing technology (NGS), aiming to find out critical genes and pathways associated with the male sterility.

Results: We generated approximately 53 million sequencing reads and assembled de novo, yielding 85,144 high quality unigenes with an average length of 643 bp. Among these unigenes, 27,191 were identified as putative homologs of annotated sequences in the public protein databases, 4,326 and 7,061 unigenes were found to be highly abundant in lines 121A and 121C, respectively. Many of the differentially expressed unigenes represent a set of potential candidate genes associated with the formation or abortion of pollen.

Conclusions: Our study profiled anther transcriptomes of a chili pepper CMS line and its restorer line. The results shed the lights on the occurrence and recovery of the disturbances in nuclear-mitochondrial interaction and provide clues for further investigations.

Show MeSH
Related in: MedlinePlus