Limits...
[18F]Fluorodeoxyglucose accumulation as a biological marker of hypoxic status but not glucose transport ability in gastric cancer.

Takebayashi R, Izuishi K, Yamamoto Y, Kameyama R, Mori H, Masaki T, Suzuki Y - J. Exp. Clin. Cancer Res. (2013)

Bottom Line: Tumor size was the only clinicopathological parameter that significantly correlated with SUV.When divided into intestinal and non-intestinal tumors, there was a significant correlation between SUV and tumor size in intestinal tumors.No correlation was found between SUV and mRNA expression of other genes in intestinal or non-intestinal tumors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gastroenterological Surgery, Kagawa University, Miki, Kita, Kagawa, Japan.

ABSTRACT

Background: The use of [18F] 2-fluoro-2-deoxy-D-glucose positron emission tomography (FDG-PET) for detection of gastric cancer is often debated because FDG uptake varies for each patient. The purpose of this study was to clarify the molecular mechanisms involved in FDG uptake.

Material and methods: Fifty patients with gastric cancer who underwent FDG-PET and gastrectomy were studied. Snap-frozen tumor specimens were collected and examined by real-time PCR for relationships between maximum standardized uptake value (SUV) and mRNA expression of the following genes: glucose transporter 1 (GLUT1), hexokinase 2 (HK2), hypoxia-inducible factor 1α (HIF1α), and proliferating cell nuclear antigen (PCNA).

Results: Tumor size was the only clinicopathological parameter that significantly correlated with SUV. Transcripts for the genes evaluated were about three-fold higher in malignant specimens than in normal mucosa, although only HIF1α was significantly correlated with SUV. When divided into intestinal and non-intestinal tumors, there was a significant correlation between SUV and tumor size in intestinal tumors. Interestingly, the weak association between SUV and HIF1α expression in intestinal tumors was substantially stronger in non-intestinal tumors. No correlation was found between SUV and mRNA expression of other genes in intestinal or non-intestinal tumors.

Conclusion: SUV was correlated with HIF1α, but not PCNA, HK2, or GLUT1 expression. FDG accumulation could therefore represent tissue hypoxia rather than glucose transport activity for aggressive cancer growth.

Show MeSH

Related in: MedlinePlus

Relationship between mean standardized uptake value and clinicopathological data in gastric cancer. (a) Mean standardized uptake value (SUV) in stage 4 gastric cancer patients was not significantly higher than in stage 2 and stage 3 patients. (b) Mean SUV in intestinal tumors was not significantly greater than in non-intestinal tumors. (c) Spearman’s correlation analysis revealed a significant correlation between tumor size and mean SUV (rs = 0.33, P < 0.05). Values are expressed as mean ± SEM. Int; Intestinal Type, Non-Int; Non-intestinal Type, SUV; Standardized Uptake Value.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3672048&req=5

Figure 1: Relationship between mean standardized uptake value and clinicopathological data in gastric cancer. (a) Mean standardized uptake value (SUV) in stage 4 gastric cancer patients was not significantly higher than in stage 2 and stage 3 patients. (b) Mean SUV in intestinal tumors was not significantly greater than in non-intestinal tumors. (c) Spearman’s correlation analysis revealed a significant correlation between tumor size and mean SUV (rs = 0.33, P < 0.05). Values are expressed as mean ± SEM. Int; Intestinal Type, Non-Int; Non-intestinal Type, SUV; Standardized Uptake Value.

Mentions: Of the 50 gastric cancer lesions, 45 showed focally increased FDG uptake. The majority of patients had advanced gastric cancer and a mean tumor size of 7.5 ± 0.5 cm, with 16 cases classified as stage 4. The mean SUV of stage 4 patients was 9.0 ± 1.3, while mean SUV of stage 2 and stage 3 patients combined was 8.3 ± 0.6 (Figure 1a). When tumors were divided into intestinal and non-intestinal tumors, mean SUVs were 7.8 ± 0.7 and 9.2 ± 1.0, respectively (Figure 1b). When divided by median lymph node metastasis, 22 cases had less than three and 28 cases had three or more; mean SUVs were not significant at 9.4 ± 1.0 and 7.8 ± 0.7, respectively. When divided by maximum median tumor diameter, 22 cases were less than 7.0 cm and 28 cases were 7.0 cm or greater; mean SUVs were 7.0 ± 0.6 and 9.7 ± 0.9, respectively (P < 0.05).


[18F]Fluorodeoxyglucose accumulation as a biological marker of hypoxic status but not glucose transport ability in gastric cancer.

Takebayashi R, Izuishi K, Yamamoto Y, Kameyama R, Mori H, Masaki T, Suzuki Y - J. Exp. Clin. Cancer Res. (2013)

Relationship between mean standardized uptake value and clinicopathological data in gastric cancer. (a) Mean standardized uptake value (SUV) in stage 4 gastric cancer patients was not significantly higher than in stage 2 and stage 3 patients. (b) Mean SUV in intestinal tumors was not significantly greater than in non-intestinal tumors. (c) Spearman’s correlation analysis revealed a significant correlation between tumor size and mean SUV (rs = 0.33, P < 0.05). Values are expressed as mean ± SEM. Int; Intestinal Type, Non-Int; Non-intestinal Type, SUV; Standardized Uptake Value.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3672048&req=5

Figure 1: Relationship between mean standardized uptake value and clinicopathological data in gastric cancer. (a) Mean standardized uptake value (SUV) in stage 4 gastric cancer patients was not significantly higher than in stage 2 and stage 3 patients. (b) Mean SUV in intestinal tumors was not significantly greater than in non-intestinal tumors. (c) Spearman’s correlation analysis revealed a significant correlation between tumor size and mean SUV (rs = 0.33, P < 0.05). Values are expressed as mean ± SEM. Int; Intestinal Type, Non-Int; Non-intestinal Type, SUV; Standardized Uptake Value.
Mentions: Of the 50 gastric cancer lesions, 45 showed focally increased FDG uptake. The majority of patients had advanced gastric cancer and a mean tumor size of 7.5 ± 0.5 cm, with 16 cases classified as stage 4. The mean SUV of stage 4 patients was 9.0 ± 1.3, while mean SUV of stage 2 and stage 3 patients combined was 8.3 ± 0.6 (Figure 1a). When tumors were divided into intestinal and non-intestinal tumors, mean SUVs were 7.8 ± 0.7 and 9.2 ± 1.0, respectively (Figure 1b). When divided by median lymph node metastasis, 22 cases had less than three and 28 cases had three or more; mean SUVs were not significant at 9.4 ± 1.0 and 7.8 ± 0.7, respectively. When divided by maximum median tumor diameter, 22 cases were less than 7.0 cm and 28 cases were 7.0 cm or greater; mean SUVs were 7.0 ± 0.6 and 9.7 ± 0.9, respectively (P < 0.05).

Bottom Line: Tumor size was the only clinicopathological parameter that significantly correlated with SUV.When divided into intestinal and non-intestinal tumors, there was a significant correlation between SUV and tumor size in intestinal tumors.No correlation was found between SUV and mRNA expression of other genes in intestinal or non-intestinal tumors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gastroenterological Surgery, Kagawa University, Miki, Kita, Kagawa, Japan.

ABSTRACT

Background: The use of [18F] 2-fluoro-2-deoxy-D-glucose positron emission tomography (FDG-PET) for detection of gastric cancer is often debated because FDG uptake varies for each patient. The purpose of this study was to clarify the molecular mechanisms involved in FDG uptake.

Material and methods: Fifty patients with gastric cancer who underwent FDG-PET and gastrectomy were studied. Snap-frozen tumor specimens were collected and examined by real-time PCR for relationships between maximum standardized uptake value (SUV) and mRNA expression of the following genes: glucose transporter 1 (GLUT1), hexokinase 2 (HK2), hypoxia-inducible factor 1α (HIF1α), and proliferating cell nuclear antigen (PCNA).

Results: Tumor size was the only clinicopathological parameter that significantly correlated with SUV. Transcripts for the genes evaluated were about three-fold higher in malignant specimens than in normal mucosa, although only HIF1α was significantly correlated with SUV. When divided into intestinal and non-intestinal tumors, there was a significant correlation between SUV and tumor size in intestinal tumors. Interestingly, the weak association between SUV and HIF1α expression in intestinal tumors was substantially stronger in non-intestinal tumors. No correlation was found between SUV and mRNA expression of other genes in intestinal or non-intestinal tumors.

Conclusion: SUV was correlated with HIF1α, but not PCNA, HK2, or GLUT1 expression. FDG accumulation could therefore represent tissue hypoxia rather than glucose transport activity for aggressive cancer growth.

Show MeSH
Related in: MedlinePlus