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VEGF-c expression in an in vivo model of orthotopic endometrial cancer and retroperitoneal lymph node metastasis.

Huang YW, Xu LQ, Luo RZ, Huang X, Hou T, Zhang YN - Reprod. Biol. Endocrinol. (2013)

Bottom Line: Control groups consisted of those receiving no treatment or an injection of saline.As compared to the control groups, VEGF-c mRNA expression increased significantly over time in the tumor site, RLN, and peripheral white blood cells of EC rabbits.Injection of a VX2 cell suspension is a simple method of establishing an in vivo EC model.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Retroperitoneal lymph node (RLN) metastasis is an important indicator of endometrial cancer (EC) prognosis. Because vascular endothelial growth factor c (VEGF-c) is known to influence lymphangiogenesis and thereby lymph node metastasis, this study assessed the relationship of VEGF-c mRNA expression with RLN metastasis in EC.

Methods: The uterine muscularis mucosae of New Zealand white rabbits were inoculated with a VX2 tumor cell suspension after which they were sacrificed at 15, 18, 21, 24, 27 and 30 days. Control groups consisted of those receiving no treatment or an injection of saline. EC and metastatic RLN tissues along with peripheral blood samples were collected, and VEGF-c mRNA expression was evaluated using fluorescence real-time quantitative PCR.

Results: The establishment of an in vivo model of EC with complete RLN metastasis was pathologically confirmed at day 21 post-injection with VX2 cells. As compared to the control groups, VEGF-c mRNA expression increased significantly over time in the tumor site, RLN, and peripheral white blood cells of EC rabbits. Significantly higher VEGF-c mRNA expression was observed in metastatic RLNs as compared to those without metastasis (P < 0.001). In addition, increased VEGF-c mRNA expression was observed in peripheral white blood cells of rabbits with RLN metastasis (P < 0.002).

Conclusion: Injection of a VX2 cell suspension is a simple method of establishing an in vivo EC model. VEGF-c may play an important role in the development of EC and its metastasis to RLN and may be useful marker to predict RLN metastasis.

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Tumor volume over time after injection with VX2 tumor cells. The size of tumors in the experimental group was determined at the indicated time points. Results represent the means ± SD at each time point (n = 6 per time point). * Indicates a statistically significant difference between the indicated group and the 15 D group, P < 0.05.
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Figure 1: Tumor volume over time after injection with VX2 tumor cells. The size of tumors in the experimental group was determined at the indicated time points. Results represent the means ± SD at each time point (n = 6 per time point). * Indicates a statistically significant difference between the indicated group and the 15 D group, P < 0.05.

Mentions: As shown in Figure 1, significantly increased tumor volume was observed at days 24, 27, and 30 post-injection of VX2 cells (P < 0.05). A representative image of the normal and tumor endometrium after 21 days is shown in Figure 2A. Histological analysis of the tumor tissue confirmed the presence of tumor cells (Figure 2B).


VEGF-c expression in an in vivo model of orthotopic endometrial cancer and retroperitoneal lymph node metastasis.

Huang YW, Xu LQ, Luo RZ, Huang X, Hou T, Zhang YN - Reprod. Biol. Endocrinol. (2013)

Tumor volume over time after injection with VX2 tumor cells. The size of tumors in the experimental group was determined at the indicated time points. Results represent the means ± SD at each time point (n = 6 per time point). * Indicates a statistically significant difference between the indicated group and the 15 D group, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3672014&req=5

Figure 1: Tumor volume over time after injection with VX2 tumor cells. The size of tumors in the experimental group was determined at the indicated time points. Results represent the means ± SD at each time point (n = 6 per time point). * Indicates a statistically significant difference between the indicated group and the 15 D group, P < 0.05.
Mentions: As shown in Figure 1, significantly increased tumor volume was observed at days 24, 27, and 30 post-injection of VX2 cells (P < 0.05). A representative image of the normal and tumor endometrium after 21 days is shown in Figure 2A. Histological analysis of the tumor tissue confirmed the presence of tumor cells (Figure 2B).

Bottom Line: Control groups consisted of those receiving no treatment or an injection of saline.As compared to the control groups, VEGF-c mRNA expression increased significantly over time in the tumor site, RLN, and peripheral white blood cells of EC rabbits.Injection of a VX2 cell suspension is a simple method of establishing an in vivo EC model.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Retroperitoneal lymph node (RLN) metastasis is an important indicator of endometrial cancer (EC) prognosis. Because vascular endothelial growth factor c (VEGF-c) is known to influence lymphangiogenesis and thereby lymph node metastasis, this study assessed the relationship of VEGF-c mRNA expression with RLN metastasis in EC.

Methods: The uterine muscularis mucosae of New Zealand white rabbits were inoculated with a VX2 tumor cell suspension after which they were sacrificed at 15, 18, 21, 24, 27 and 30 days. Control groups consisted of those receiving no treatment or an injection of saline. EC and metastatic RLN tissues along with peripheral blood samples were collected, and VEGF-c mRNA expression was evaluated using fluorescence real-time quantitative PCR.

Results: The establishment of an in vivo model of EC with complete RLN metastasis was pathologically confirmed at day 21 post-injection with VX2 cells. As compared to the control groups, VEGF-c mRNA expression increased significantly over time in the tumor site, RLN, and peripheral white blood cells of EC rabbits. Significantly higher VEGF-c mRNA expression was observed in metastatic RLNs as compared to those without metastasis (P < 0.001). In addition, increased VEGF-c mRNA expression was observed in peripheral white blood cells of rabbits with RLN metastasis (P < 0.002).

Conclusion: Injection of a VX2 cell suspension is a simple method of establishing an in vivo EC model. VEGF-c may play an important role in the development of EC and its metastasis to RLN and may be useful marker to predict RLN metastasis.

Show MeSH
Related in: MedlinePlus