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Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro.

Zhang ZM, Yang XM, Zhang C, Zhang MJ, Li X, Zhang FH, Kang S, Wang SJ, Shan BE - Onco Targets Ther (2013)

Bottom Line: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05).A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.

ABSTRACT

Objective: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.

Methods: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy.

Results: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05).

Conclusion: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

No MeSH data available.


Related in: MedlinePlus

Proliferation of allogenetic T lymphocytes induced by dendritic cells (DCs) stimulated with or without sCD40L.Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L, soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.
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f7-ott-6-503: Proliferation of allogenetic T lymphocytes induced by dendritic cells (DCs) stimulated with or without sCD40L.Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L, soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.

Mentions: DCs, cultured for 5 days, were collected as stimulator cells and co-cultured with homogeneity variant T cells and then T lymphocyte proliferation was detected via MTT assay. MTT assay revealed that the proliferation ability of T cells stimulated with GM-CSF+IL-4+SCF+Flt-3l+sCD40L-induced DCs was significantly higher than other groups (P < 0.05). The proliferation ability of T cells stimulated with GM-CSF+IL-4+TNF-α-induced DCs was similar to that of those stimulated with GM-CSF+IL-4+SCF+Flt-3l+TNF-α-induced DCs, but both were significantly higher than that of those stimulated with GM-CSF+IL-4-induced DCs (P < 0.05). Moreover, the proliferation ability of T cells was stronger and the SI was 2.41 ± 0.11 – this was highest when DCs were co-cultured with homogeneity variant T cells in a ratio of 1:10 and was significantly higher than that observed with the ratios 1:20 (SI = 1.04 ± 0.06) and 1:40 (SI = 0.61 ± 0.06) (Figure 7).


Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro.

Zhang ZM, Yang XM, Zhang C, Zhang MJ, Li X, Zhang FH, Kang S, Wang SJ, Shan BE - Onco Targets Ther (2013)

Proliferation of allogenetic T lymphocytes induced by dendritic cells (DCs) stimulated with or without sCD40L.Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L, soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649858&req=5

f7-ott-6-503: Proliferation of allogenetic T lymphocytes induced by dendritic cells (DCs) stimulated with or without sCD40L.Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L, soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.
Mentions: DCs, cultured for 5 days, were collected as stimulator cells and co-cultured with homogeneity variant T cells and then T lymphocyte proliferation was detected via MTT assay. MTT assay revealed that the proliferation ability of T cells stimulated with GM-CSF+IL-4+SCF+Flt-3l+sCD40L-induced DCs was significantly higher than other groups (P < 0.05). The proliferation ability of T cells stimulated with GM-CSF+IL-4+TNF-α-induced DCs was similar to that of those stimulated with GM-CSF+IL-4+SCF+Flt-3l+TNF-α-induced DCs, but both were significantly higher than that of those stimulated with GM-CSF+IL-4-induced DCs (P < 0.05). Moreover, the proliferation ability of T cells was stronger and the SI was 2.41 ± 0.11 – this was highest when DCs were co-cultured with homogeneity variant T cells in a ratio of 1:10 and was significantly higher than that observed with the ratios 1:20 (SI = 1.04 ± 0.06) and 1:40 (SI = 0.61 ± 0.06) (Figure 7).

Bottom Line: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05).A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.

ABSTRACT

Objective: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.

Methods: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy.

Results: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05).

Conclusion: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

No MeSH data available.


Related in: MedlinePlus