Limits...
Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro.

Zhang ZM, Yang XM, Zhang C, Zhang MJ, Li X, Zhang FH, Kang S, Wang SJ, Shan BE - Onco Targets Ther (2013)

Bottom Line: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05).A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.

ABSTRACT

Objective: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.

Methods: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy.

Results: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05).

Conclusion: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

No MeSH data available.


Related in: MedlinePlus

Effect of different cytokines on the morphology of dendritic cells derived from cord blood. (A1) GM-CSF+IL-4 cultured for 5 days (×200); (A2) GM-CSF+IL-4 cultured for 7 days (×400); (B1) GM-CSF+IL-4+TNF-α cultured for 5 days (×200); (B2) GM-CSF+IL-4+TNF-α cultured for 7 days (×400); (C1) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 5 days (×200); (C2) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 7 days (×400); (D1 ) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 5 days (×200); (D2) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 7 days (×400).Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3649858&req=5

f2-ott-6-503: Effect of different cytokines on the morphology of dendritic cells derived from cord blood. (A1) GM-CSF+IL-4 cultured for 5 days (×200); (A2) GM-CSF+IL-4 cultured for 7 days (×400); (B1) GM-CSF+IL-4+TNF-α cultured for 5 days (×200); (B2) GM-CSF+IL-4+TNF-α cultured for 7 days (×400); (C1) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 5 days (×200); (C2) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 7 days (×400); (D1 ) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 5 days (×200); (D2) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 7 days (×400).Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.

Mentions: During the process of differentiation of CBMCs into DCs, morphological changes in DCs were induced by different cytokine combinations. On the first day, the majority of cells were round and adherent cells. On the third day, floating cells were observed and clusters of adherent cells began to gather and form colonies. On the fifth day, the floating cells had gradually increased in the sCD40L-induced group, were irregular in shape, and had enlarged volume, finally growing into colonies. On the seventh day, the clusters of cells were scattered and prominent burr-like protuberances were observed on the surface of the enlarged cells. Similar morphological changes were observed in cells in the TNF-α-induced group, while the cells in the immature DC (GM-CSF+IL-4) group were smaller and had fewer surface protrusions. On the seventh day, some of the cells had spindle-macrophage cell morphology. The DCs were significantly induced in those groups treated with SCF + Flt-3L compared to the untreated groups (Figure 2).


Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro.

Zhang ZM, Yang XM, Zhang C, Zhang MJ, Li X, Zhang FH, Kang S, Wang SJ, Shan BE - Onco Targets Ther (2013)

Effect of different cytokines on the morphology of dendritic cells derived from cord blood. (A1) GM-CSF+IL-4 cultured for 5 days (×200); (A2) GM-CSF+IL-4 cultured for 7 days (×400); (B1) GM-CSF+IL-4+TNF-α cultured for 5 days (×200); (B2) GM-CSF+IL-4+TNF-α cultured for 7 days (×400); (C1) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 5 days (×200); (C2) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 7 days (×400); (D1 ) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 5 days (×200); (D2) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 7 days (×400).Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649858&req=5

f2-ott-6-503: Effect of different cytokines on the morphology of dendritic cells derived from cord blood. (A1) GM-CSF+IL-4 cultured for 5 days (×200); (A2) GM-CSF+IL-4 cultured for 7 days (×400); (B1) GM-CSF+IL-4+TNF-α cultured for 5 days (×200); (B2) GM-CSF+IL-4+TNF-α cultured for 7 days (×400); (C1) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 5 days (×200); (C2) GM-CSF+IL-4+SCF+Flt-3l+TNF-α cultured for 7 days (×400); (D1 ) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 5 days (×200); (D2) GM-CSF+IL-4+SCF+Flt-3l+sCD40L cultured for 7 days (×400).Abbreviations: Flt-3l, Flt-3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; sCD40L soluble cluster of differentiation 40 ligand; SCF, stem cell factor; TNF, tumor necrosis factor.
Mentions: During the process of differentiation of CBMCs into DCs, morphological changes in DCs were induced by different cytokine combinations. On the first day, the majority of cells were round and adherent cells. On the third day, floating cells were observed and clusters of adherent cells began to gather and form colonies. On the fifth day, the floating cells had gradually increased in the sCD40L-induced group, were irregular in shape, and had enlarged volume, finally growing into colonies. On the seventh day, the clusters of cells were scattered and prominent burr-like protuberances were observed on the surface of the enlarged cells. Similar morphological changes were observed in cells in the TNF-α-induced group, while the cells in the immature DC (GM-CSF+IL-4) group were smaller and had fewer surface protrusions. On the seventh day, some of the cells had spindle-macrophage cell morphology. The DCs were significantly induced in those groups treated with SCF + Flt-3L compared to the untreated groups (Figure 2).

Bottom Line: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05).A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.

ABSTRACT

Objective: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients.

Methods: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy.

Results: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05).

Conclusion: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.

No MeSH data available.


Related in: MedlinePlus