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Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus

Autoradiography analysis of the RNA synthesis rate in a reprogramming oligodendrocytic nucleus. (a) Here, 5 neuronal nuclei and 6 oligodendrocytic nuclei can be observed in the field of view. (b) The same section area in transmitted light. All 5 neuronal nuclei are intensely marked by silver grains, while none of the 6 oligodendrocytic nuclei contain this label. (c) Another region of the preparation. In the field of view, 4 oligodendrocytic nuclei can be observed. A proneuronal change in the structure, with dark areas of euchromatin, is observed in the nucleus (arrow) that is the largest and is colored irregularly. (d) The same section area in transmitted light. The proneuronal structural change has a functional manifestation: the appearance of the label over the oligodendrocytic nucleus (marked by an arrow) indicates an increase in the RNA synthesis rate. (a) and (c) are stained with DAPI. (b) and (d) show manifestation of the autoradiographic label (silver grains). The scale bar is 15 μm.
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fig12: Autoradiography analysis of the RNA synthesis rate in a reprogramming oligodendrocytic nucleus. (a) Here, 5 neuronal nuclei and 6 oligodendrocytic nuclei can be observed in the field of view. (b) The same section area in transmitted light. All 5 neuronal nuclei are intensely marked by silver grains, while none of the 6 oligodendrocytic nuclei contain this label. (c) Another region of the preparation. In the field of view, 4 oligodendrocytic nuclei can be observed. A proneuronal change in the structure, with dark areas of euchromatin, is observed in the nucleus (arrow) that is the largest and is colored irregularly. (d) The same section area in transmitted light. The proneuronal structural change has a functional manifestation: the appearance of the label over the oligodendrocytic nucleus (marked by an arrow) indicates an increase in the RNA synthesis rate. (a) and (c) are stained with DAPI. (b) and (d) show manifestation of the autoradiographic label (silver grains). The scale bar is 15 μm.

Mentions: The results of autoradiographic experiments are presented in Figure 12, showing an oligodendrocyte's nucleus with a proneuronal alteration in structure (chromatin separation) that changed the function in the proneuronal direction; revealingly, it was labeled as the neuronal nucleus. The transcription rate increased to that of a neuron.


Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Autoradiography analysis of the RNA synthesis rate in a reprogramming oligodendrocytic nucleus. (a) Here, 5 neuronal nuclei and 6 oligodendrocytic nuclei can be observed in the field of view. (b) The same section area in transmitted light. All 5 neuronal nuclei are intensely marked by silver grains, while none of the 6 oligodendrocytic nuclei contain this label. (c) Another region of the preparation. In the field of view, 4 oligodendrocytic nuclei can be observed. A proneuronal change in the structure, with dark areas of euchromatin, is observed in the nucleus (arrow) that is the largest and is colored irregularly. (d) The same section area in transmitted light. The proneuronal structural change has a functional manifestation: the appearance of the label over the oligodendrocytic nucleus (marked by an arrow) indicates an increase in the RNA synthesis rate. (a) and (c) are stained with DAPI. (b) and (d) show manifestation of the autoradiographic label (silver grains). The scale bar is 15 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649807&req=5

fig12: Autoradiography analysis of the RNA synthesis rate in a reprogramming oligodendrocytic nucleus. (a) Here, 5 neuronal nuclei and 6 oligodendrocytic nuclei can be observed in the field of view. (b) The same section area in transmitted light. All 5 neuronal nuclei are intensely marked by silver grains, while none of the 6 oligodendrocytic nuclei contain this label. (c) Another region of the preparation. In the field of view, 4 oligodendrocytic nuclei can be observed. A proneuronal change in the structure, with dark areas of euchromatin, is observed in the nucleus (arrow) that is the largest and is colored irregularly. (d) The same section area in transmitted light. The proneuronal structural change has a functional manifestation: the appearance of the label over the oligodendrocytic nucleus (marked by an arrow) indicates an increase in the RNA synthesis rate. (a) and (c) are stained with DAPI. (b) and (d) show manifestation of the autoradiographic label (silver grains). The scale bar is 15 μm.
Mentions: The results of autoradiographic experiments are presented in Figure 12, showing an oligodendrocyte's nucleus with a proneuronal alteration in structure (chromatin separation) that changed the function in the proneuronal direction; revealingly, it was labeled as the neuronal nucleus. The transcription rate increased to that of a neuron.

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus