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Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus

MAP2 expression in a heterokaryon. (a) Neuronal nucleus (larger and darker) and an adjacent reprogramming oligodendrocytic nucleus (smaller in size and brighter). (b) The same section area under a green filter. The circle of green matter (MAP2 immunoreactivity) surrounds the nuclei of both the neuron and the oligodendrocyte. (a) DAPI staining. (b) Immunocytochemical detection of MAP2 labeled by DyLight 488. The scale bar is 10 μm.
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fig11: MAP2 expression in a heterokaryon. (a) Neuronal nucleus (larger and darker) and an adjacent reprogramming oligodendrocytic nucleus (smaller in size and brighter). (b) The same section area under a green filter. The circle of green matter (MAP2 immunoreactivity) surrounds the nuclei of both the neuron and the oligodendrocyte. (a) DAPI staining. (b) Immunocytochemical detection of MAP2 labeled by DyLight 488. The scale bar is 10 μm.

Mentions: We also studied the expression of another neuronal marker, MAP2, in a heterokaryon (Figure 11).


Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

MAP2 expression in a heterokaryon. (a) Neuronal nucleus (larger and darker) and an adjacent reprogramming oligodendrocytic nucleus (smaller in size and brighter). (b) The same section area under a green filter. The circle of green matter (MAP2 immunoreactivity) surrounds the nuclei of both the neuron and the oligodendrocyte. (a) DAPI staining. (b) Immunocytochemical detection of MAP2 labeled by DyLight 488. The scale bar is 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649807&req=5

fig11: MAP2 expression in a heterokaryon. (a) Neuronal nucleus (larger and darker) and an adjacent reprogramming oligodendrocytic nucleus (smaller in size and brighter). (b) The same section area under a green filter. The circle of green matter (MAP2 immunoreactivity) surrounds the nuclei of both the neuron and the oligodendrocyte. (a) DAPI staining. (b) Immunocytochemical detection of MAP2 labeled by DyLight 488. The scale bar is 10 μm.
Mentions: We also studied the expression of another neuronal marker, MAP2, in a heterokaryon (Figure 11).

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus