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Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus

Morphological characteristics of group 5. (a) Group 5. Infiltrate. Newly formed vessels and macrophages. The vessels are marked with arrows. Semi-thin section. (b) Group 5. Penumbra. Newly generated vessels are marked with arrows. Semi-thin section. (c) Group 5. Damaged area. Newly generated vessels with a large diameter are observed in the infiltrate and penumbra. The vessels are marked with black arrows; the red dashed line bounds the infiltration zone, and the blue line denotes the penumbra. (d) Group 5. Infiltration. Small number of macrophages with green granules.  The scale bars are 20 μm in (a) and (d), 100 μm in (b), and 300 μm in (c).
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fig7: Morphological characteristics of group 5. (a) Group 5. Infiltrate. Newly formed vessels and macrophages. The vessels are marked with arrows. Semi-thin section. (b) Group 5. Penumbra. Newly generated vessels are marked with arrows. Semi-thin section. (c) Group 5. Damaged area. Newly generated vessels with a large diameter are observed in the infiltrate and penumbra. The vessels are marked with black arrows; the red dashed line bounds the infiltration zone, and the blue line denotes the penumbra. (d) Group 5. Infiltration. Small number of macrophages with green granules.  The scale bars are 20 μm in (a) and (d), 100 μm in (b), and 300 μm in (c).

Mentions: Animals of experimental group 5 had a higher number of newly formed vessels that were found in both the infiltrate and penumbra (Figures 7(a), 7(b), and 7(c)). The infiltrates mainly contained newly formed capillary tubes and sinusoid venules (Figure 7(a)) with extremely thin walls. Arterioles and more mature vessels were found in the penumbra (Figures 7(b) and 7(c)).


Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Morphological characteristics of group 5. (a) Group 5. Infiltrate. Newly formed vessels and macrophages. The vessels are marked with arrows. Semi-thin section. (b) Group 5. Penumbra. Newly generated vessels are marked with arrows. Semi-thin section. (c) Group 5. Damaged area. Newly generated vessels with a large diameter are observed in the infiltrate and penumbra. The vessels are marked with black arrows; the red dashed line bounds the infiltration zone, and the blue line denotes the penumbra. (d) Group 5. Infiltration. Small number of macrophages with green granules.  The scale bars are 20 μm in (a) and (d), 100 μm in (b), and 300 μm in (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649807&req=5

fig7: Morphological characteristics of group 5. (a) Group 5. Infiltrate. Newly formed vessels and macrophages. The vessels are marked with arrows. Semi-thin section. (b) Group 5. Penumbra. Newly generated vessels are marked with arrows. Semi-thin section. (c) Group 5. Damaged area. Newly generated vessels with a large diameter are observed in the infiltrate and penumbra. The vessels are marked with black arrows; the red dashed line bounds the infiltration zone, and the blue line denotes the penumbra. (d) Group 5. Infiltration. Small number of macrophages with green granules.  The scale bars are 20 μm in (a) and (d), 100 μm in (b), and 300 μm in (c).
Mentions: Animals of experimental group 5 had a higher number of newly formed vessels that were found in both the infiltrate and penumbra (Figures 7(a), 7(b), and 7(c)). The infiltrates mainly contained newly formed capillary tubes and sinusoid venules (Figure 7(a)) with extremely thin walls. Arterioles and more mature vessels were found in the penumbra (Figures 7(b) and 7(c)).

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus