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Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus

Morphological characteristics of group 4. (a) Group 4: infiltrate. Numerous cells of infiltrate and newly formed vessels. Arterioles are marked with arrows. (b) Group 4: penumbra. Extravasate formed by separated erythrocytes at the boundary between the penumbra and infiltrate. (c) Group 4: infiltrate. Macrophages with pale and stained granules. (d) Group 4: penumbra. Newly formed blood vessel is marked with a red arrow, and macrophages in the vessel wall are marked with black arrows. Hypertrophied vessel wall containing macrophages with dark cytoplasm. Semi-thin section. The scale bars are 100 μm in (a) and (b), 30 μm in (c), and 20 μm in (d).
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fig6: Morphological characteristics of group 4. (a) Group 4: infiltrate. Numerous cells of infiltrate and newly formed vessels. Arterioles are marked with arrows. (b) Group 4: penumbra. Extravasate formed by separated erythrocytes at the boundary between the penumbra and infiltrate. (c) Group 4: infiltrate. Macrophages with pale and stained granules. (d) Group 4: penumbra. Newly formed blood vessel is marked with a red arrow, and macrophages in the vessel wall are marked with black arrows. Hypertrophied vessel wall containing macrophages with dark cytoplasm. Semi-thin section. The scale bars are 100 μm in (a) and (b), 30 μm in (c), and 20 μm in (d).

Mentions: Group 4 (animals subjected to 7-day AOH before the injury) was characterized by a higher quantity of newly formed vessels compared to groups 1 and 3 (Figure 6(a)). The vessels were represented not only by capillary tubes but also by more mature venules and arterioles (Figures 6(a) and 6(d)).


Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Morphological characteristics of group 4. (a) Group 4: infiltrate. Numerous cells of infiltrate and newly formed vessels. Arterioles are marked with arrows. (b) Group 4: penumbra. Extravasate formed by separated erythrocytes at the boundary between the penumbra and infiltrate. (c) Group 4: infiltrate. Macrophages with pale and stained granules. (d) Group 4: penumbra. Newly formed blood vessel is marked with a red arrow, and macrophages in the vessel wall are marked with black arrows. Hypertrophied vessel wall containing macrophages with dark cytoplasm. Semi-thin section. The scale bars are 100 μm in (a) and (b), 30 μm in (c), and 20 μm in (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3649807&req=5

fig6: Morphological characteristics of group 4. (a) Group 4: infiltrate. Numerous cells of infiltrate and newly formed vessels. Arterioles are marked with arrows. (b) Group 4: penumbra. Extravasate formed by separated erythrocytes at the boundary between the penumbra and infiltrate. (c) Group 4: infiltrate. Macrophages with pale and stained granules. (d) Group 4: penumbra. Newly formed blood vessel is marked with a red arrow, and macrophages in the vessel wall are marked with black arrows. Hypertrophied vessel wall containing macrophages with dark cytoplasm. Semi-thin section. The scale bars are 100 μm in (a) and (b), 30 μm in (c), and 20 μm in (d).
Mentions: Group 4 (animals subjected to 7-day AOH before the injury) was characterized by a higher quantity of newly formed vessels compared to groups 1 and 3 (Figure 6(a)). The vessels were represented not only by capillary tubes but also by more mature venules and arterioles (Figures 6(a) and 6(d)).

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus