Limits...
Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus

Simulation of microgravitation. Rat from experimental group 4. 1st day of AOH.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3649807&req=5

fig2: Simulation of microgravitation. Rat from experimental group 4. 1st day of AOH.

Mentions: Microgravity conditions were simulated by unloading the hind limbs fully and the fore limbs partially by modulation of antiorthostatic hypokinesia (AOH). To simulate microgravity, the rats were equipped with special suits and suspended head down at an angle of 30° in individual cages. The suits were made of synthetic fabric with holes for the fore and hind limbs. A metal plate was stitched into the suit fabric of the dorsal area to provide a straight spine position and to prevent spine wriggle during the experiment. The cage had plexiglass walls and a net floor. The suspension loop was fixed on a block that was freely moved along a joist in the ceiling. This apparatus allowed animals to move easily in the cage and to have full-time access to food and water. Because the hind limbs did not contact the floor, the animals moved using their fore limbs (Figure 2).


Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Paltsyn A, Komissarova S, Dubrovin I, Kubatiev A - Stroke Res Treat (2013)

Simulation of microgravitation. Rat from experimental group 4. 1st day of AOH.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649807&req=5

fig2: Simulation of microgravitation. Rat from experimental group 4. 1st day of AOH.
Mentions: Microgravity conditions were simulated by unloading the hind limbs fully and the fore limbs partially by modulation of antiorthostatic hypokinesia (AOH). To simulate microgravity, the rats were equipped with special suits and suspended head down at an angle of 30° in individual cages. The suits were made of synthetic fabric with holes for the fore and hind limbs. A metal plate was stitched into the suit fabric of the dorsal area to provide a straight spine position and to prevent spine wriggle during the experiment. The cage had plexiglass walls and a net floor. The suspension loop was fixed on a block that was freely moved along a joist in the ceiling. This apparatus allowed animals to move easily in the cage and to have full-time access to food and water. Because the hind limbs did not contact the floor, the animals moved using their fore limbs (Figure 2).

Bottom Line: The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons.Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons.In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

View Article: PubMed Central - PubMed

Affiliation: Institute of General Pathology and Pathophysiology of the Russian Academy of Medical Sciences, Baltiskaya Street 8, Moscow 125315, Russia ; Russian Medical Academy of Postgraduate Education, Moscow, Russia.

ABSTRACT
In this study, we used a model of a hemorrhagic stroke in a motor zone of the cortex in rats at the age of 3 months The report shows that cortical neurons can fuse with oligodendrocytes. In formed binuclear cells, the nucleus of an oligodendrocyte undergoes neuron specific reprogramming. It can be confirmed by changes in chromatin structure and in size of the second nucleus, by expression of specific neuronal markers and increasing total transcription rate. The nucleus of an oligodendrocyte likely transforms into a second neuronal nucleus. The number of binuclear neurons was validated with quantitative analysis. Fusion of neurons with oligodendrocytes might be a regenerative process in general and specifically following a stroke. The appearance of additional neuronal nuclei increases the functional outcome of the population of neurons. Participation of a certain number of binuclear cells in neuronal function might compensate for a functional deficit that arises from the death of a subset of neurons. After a stroke, the number of binuclear neurons increased in cortex around the lesion zone. In this case, the rate of recovery of stroke-damaged locomotor behavior also increased, which indicates the regenerative role of fusion.

No MeSH data available.


Related in: MedlinePlus