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The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus

Analysis of LXR and RXRα coexpression in extravillous trophoblast cells (EVT) of abortive tissue. LXR expression is shown in red (a). RXRα expression is visualized in green (b). Synchronized expression of both nuclear receptors in EVT is shown in (c). The same expression scheme was identified in normal control decidua.
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fig6: Analysis of LXR and RXRα coexpression in extravillous trophoblast cells (EVT) of abortive tissue. LXR expression is shown in red (a). RXRα expression is visualized in green (b). Synchronized expression of both nuclear receptors in EVT is shown in (c). The same expression scheme was identified in normal control decidua.

Mentions: In this study, EVT in placentas from miscarriages expressed LXR, stained in red (Figure 6(a)), and they expressed RXRα, stained in green (Figure 6(b)). Triple filter excitation showed expression of LXR and RXRα in the same EVT, indicated by yellow staining. Hence, a coexpression of LXR and RXRα could be shown in placentas of spontaneous miscarriages (Figure 6(c)). The same applied for the normal control decidua.


The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Analysis of LXR and RXRα coexpression in extravillous trophoblast cells (EVT) of abortive tissue. LXR expression is shown in red (a). RXRα expression is visualized in green (b). Synchronized expression of both nuclear receptors in EVT is shown in (c). The same expression scheme was identified in normal control decidua.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649716&req=5

fig6: Analysis of LXR and RXRα coexpression in extravillous trophoblast cells (EVT) of abortive tissue. LXR expression is shown in red (a). RXRα expression is visualized in green (b). Synchronized expression of both nuclear receptors in EVT is shown in (c). The same expression scheme was identified in normal control decidua.
Mentions: In this study, EVT in placentas from miscarriages expressed LXR, stained in red (Figure 6(a)), and they expressed RXRα, stained in green (Figure 6(b)). Triple filter excitation showed expression of LXR and RXRα in the same EVT, indicated by yellow staining. Hence, a coexpression of LXR and RXRα could be shown in placentas of spontaneous miscarriages (Figure 6(c)). The same applied for the normal control decidua.

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus