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The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus

Identification of cells in the abortive decidua expressing nuclear receptors. Expression of LXR (a), RXRα (d), and, PPARγ (g) is shown in red. Expression of HLA-G as a marker for extravillous trophoblast cells is shown in green ((b), (e), (h), resp.). Coexpression of both, nuclear receptor in red and HLA-G in green, indicates extravillous trophoblast cells expressing LXR (c), RXRα (f), and PPARγ (i). The same expression scheme was identified in normal control decidua.
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fig5: Identification of cells in the abortive decidua expressing nuclear receptors. Expression of LXR (a), RXRα (d), and, PPARγ (g) is shown in red. Expression of HLA-G as a marker for extravillous trophoblast cells is shown in green ((b), (e), (h), resp.). Coexpression of both, nuclear receptor in red and HLA-G in green, indicates extravillous trophoblast cells expressing LXR (c), RXRα (f), and PPARγ (i). The same expression scheme was identified in normal control decidua.

Mentions: HLA-G was used as antigen that is expressed exclusively in extavillous trophoblast cells. Expression of LXR, RXRα, and PPARγ is shown in Figures 5(a), 5(d), and 5(g), respectively, in abortive tissue. The pictures showing expression of HLA-G are shown in Figures 5(b), 5(e) and 5(h), respectively. Double-immunofluorescence staining as shown in Figures 5(c), 5(f), and 5(i), respectively, indicates synchronous expression of both nuclear receptor and HLA-G in the placentas of miscarriages. We identified the same expression scheme in normal control decidua.


The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Identification of cells in the abortive decidua expressing nuclear receptors. Expression of LXR (a), RXRα (d), and, PPARγ (g) is shown in red. Expression of HLA-G as a marker for extravillous trophoblast cells is shown in green ((b), (e), (h), resp.). Coexpression of both, nuclear receptor in red and HLA-G in green, indicates extravillous trophoblast cells expressing LXR (c), RXRα (f), and PPARγ (i). The same expression scheme was identified in normal control decidua.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649716&req=5

fig5: Identification of cells in the abortive decidua expressing nuclear receptors. Expression of LXR (a), RXRα (d), and, PPARγ (g) is shown in red. Expression of HLA-G as a marker for extravillous trophoblast cells is shown in green ((b), (e), (h), resp.). Coexpression of both, nuclear receptor in red and HLA-G in green, indicates extravillous trophoblast cells expressing LXR (c), RXRα (f), and PPARγ (i). The same expression scheme was identified in normal control decidua.
Mentions: HLA-G was used as antigen that is expressed exclusively in extavillous trophoblast cells. Expression of LXR, RXRα, and PPARγ is shown in Figures 5(a), 5(d), and 5(g), respectively, in abortive tissue. The pictures showing expression of HLA-G are shown in Figures 5(b), 5(e) and 5(h), respectively. Double-immunofluorescence staining as shown in Figures 5(c), 5(f), and 5(i), respectively, indicates synchronous expression of both nuclear receptor and HLA-G in the placentas of miscarriages. We identified the same expression scheme in normal control decidua.

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus