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The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus

Serial sections of LXR ((a), (b)), RXRα ((c), (d)), and PPARγ ((e), (f)) expression in decidua of placentas from regular pregnancies ((a), (c), (e)) and in placenta of miscarriage ((b), (d), (f)). All magnifications are 20x lens. Box plots represent the LXR, RXR, and PPAR expression in decidua of spontaneous abortion and control decidua derived from serial sections (g). The boxes display the range between the 25th and 75th percentile and the horizontal line indicates the median. The bars represent the 5th and 95th percentiles. Circles indicate values which are more than 1.5 times the box length. Asterisks indicate values which are more than 2 times the box length. Isotypic controls for the antibodies are shown in Figure 3.
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fig4: Serial sections of LXR ((a), (b)), RXRα ((c), (d)), and PPARγ ((e), (f)) expression in decidua of placentas from regular pregnancies ((a), (c), (e)) and in placenta of miscarriage ((b), (d), (f)). All magnifications are 20x lens. Box plots represent the LXR, RXR, and PPAR expression in decidua of spontaneous abortion and control decidua derived from serial sections (g). The boxes display the range between the 25th and 75th percentile and the horizontal line indicates the median. The bars represent the 5th and 95th percentiles. Circles indicate values which are more than 1.5 times the box length. Asterisks indicate values which are more than 2 times the box length. Isotypic controls for the antibodies are shown in Figure 3.

Mentions: Serial sections were used to identify synchronized expression of LXR, RXRα, and PPARγ. We identified the expression of LXR, RXRα, and PPARγ in the nuclei of the syncytiotrophoblast (Figure 3) and decidual cells (Figure 4) of spontaneous miscarriages ((b), (d), (f)) and of first trimester control placentas ((a), (c), (e), all gestational week 7–12). In serial sections besides of reduced expression for LXR in abortive tissue, a significant increase in RXRα expression could be observed for the syncytiotrophoblast of miscarriages (IRS 2 for miscarriages, IRS 1 for control placentas, P = 0.003). In decidual cells of early pregnancy miscarriages the RXRα expression was also increased in comparison to control placentas (IRS 3 for miscarriages versus IRS 2 for control placentas, P = 0.006). PPARγ expression in the syncytiotrophoblast of miscarriages was increased in comparison to the control.


The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Serial sections of LXR ((a), (b)), RXRα ((c), (d)), and PPARγ ((e), (f)) expression in decidua of placentas from regular pregnancies ((a), (c), (e)) and in placenta of miscarriage ((b), (d), (f)). All magnifications are 20x lens. Box plots represent the LXR, RXR, and PPAR expression in decidua of spontaneous abortion and control decidua derived from serial sections (g). The boxes display the range between the 25th and 75th percentile and the horizontal line indicates the median. The bars represent the 5th and 95th percentiles. Circles indicate values which are more than 1.5 times the box length. Asterisks indicate values which are more than 2 times the box length. Isotypic controls for the antibodies are shown in Figure 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3649716&req=5

fig4: Serial sections of LXR ((a), (b)), RXRα ((c), (d)), and PPARγ ((e), (f)) expression in decidua of placentas from regular pregnancies ((a), (c), (e)) and in placenta of miscarriage ((b), (d), (f)). All magnifications are 20x lens. Box plots represent the LXR, RXR, and PPAR expression in decidua of spontaneous abortion and control decidua derived from serial sections (g). The boxes display the range between the 25th and 75th percentile and the horizontal line indicates the median. The bars represent the 5th and 95th percentiles. Circles indicate values which are more than 1.5 times the box length. Asterisks indicate values which are more than 2 times the box length. Isotypic controls for the antibodies are shown in Figure 3.
Mentions: Serial sections were used to identify synchronized expression of LXR, RXRα, and PPARγ. We identified the expression of LXR, RXRα, and PPARγ in the nuclei of the syncytiotrophoblast (Figure 3) and decidual cells (Figure 4) of spontaneous miscarriages ((b), (d), (f)) and of first trimester control placentas ((a), (c), (e), all gestational week 7–12). In serial sections besides of reduced expression for LXR in abortive tissue, a significant increase in RXRα expression could be observed for the syncytiotrophoblast of miscarriages (IRS 2 for miscarriages, IRS 1 for control placentas, P = 0.003). In decidual cells of early pregnancy miscarriages the RXRα expression was also increased in comparison to control placentas (IRS 3 for miscarriages versus IRS 2 for control placentas, P = 0.006). PPARγ expression in the syncytiotrophoblast of miscarriages was increased in comparison to the control.

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus