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The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus

Quantification of mRNA expression of LXR A and B in placentas of spontaneous miscarriages and recurrent miscarriage. (a) We identified a nonsignificant downregulation of LXR mRNA expression in spontaneous abortion placentas compared to normal controls (57%, P = 0.064). (b) In recurrent abortion we identified a downregulation of LXR mRNA expression to 53% compared to control placentas. (c) LXR A was downregulated to 27% (P = 0.07) and (e) LXR B was downregulated to 38% (P = 0.795). (d) LXR A was downregulated to 55% (P = 0.07) and (f) LXR B was downregulated to 52% (P = 0.02). Mann-Whitney-U test was used for evaluation of two independent groups.
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fig2: Quantification of mRNA expression of LXR A and B in placentas of spontaneous miscarriages and recurrent miscarriage. (a) We identified a nonsignificant downregulation of LXR mRNA expression in spontaneous abortion placentas compared to normal controls (57%, P = 0.064). (b) In recurrent abortion we identified a downregulation of LXR mRNA expression to 53% compared to control placentas. (c) LXR A was downregulated to 27% (P = 0.07) and (e) LXR B was downregulated to 38% (P = 0.795). (d) LXR A was downregulated to 55% (P = 0.07) and (f) LXR B was downregulated to 52% (P = 0.02). Mann-Whitney-U test was used for evaluation of two independent groups.

Mentions: Results of quantitative real time RT-PCR (TaqMan) showed that LXR mRNA expression was reduced in placentas of spontaneous miscariages in comparison to healthy villous tissue (Figure 2(a)) however the reduction was not significant (P = 0.064). In recurrent miscarriages, a significant reduction in LXR mRNA expression compared to control placentas could be shown (P = 0.003) (Figure 2(b)). For quantification of LXR mRNA expression, mean values for LXRA and LXRB were used.


The liver x receptor in correlation with other nuclear receptors in spontaneous and recurrent abortions.

Knabl J, Pestka A, Hüttenbrenner R, Plösch T, Ensenauer R, Welbergen L, Hutter S, Günthner-Biller M, Jeschke U - PPAR Res (2013)

Quantification of mRNA expression of LXR A and B in placentas of spontaneous miscarriages and recurrent miscarriage. (a) We identified a nonsignificant downregulation of LXR mRNA expression in spontaneous abortion placentas compared to normal controls (57%, P = 0.064). (b) In recurrent abortion we identified a downregulation of LXR mRNA expression to 53% compared to control placentas. (c) LXR A was downregulated to 27% (P = 0.07) and (e) LXR B was downregulated to 38% (P = 0.795). (d) LXR A was downregulated to 55% (P = 0.07) and (f) LXR B was downregulated to 52% (P = 0.02). Mann-Whitney-U test was used for evaluation of two independent groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649716&req=5

fig2: Quantification of mRNA expression of LXR A and B in placentas of spontaneous miscarriages and recurrent miscarriage. (a) We identified a nonsignificant downregulation of LXR mRNA expression in spontaneous abortion placentas compared to normal controls (57%, P = 0.064). (b) In recurrent abortion we identified a downregulation of LXR mRNA expression to 53% compared to control placentas. (c) LXR A was downregulated to 27% (P = 0.07) and (e) LXR B was downregulated to 38% (P = 0.795). (d) LXR A was downregulated to 55% (P = 0.07) and (f) LXR B was downregulated to 52% (P = 0.02). Mann-Whitney-U test was used for evaluation of two independent groups.
Mentions: Results of quantitative real time RT-PCR (TaqMan) showed that LXR mRNA expression was reduced in placentas of spontaneous miscariages in comparison to healthy villous tissue (Figure 2(a)) however the reduction was not significant (P = 0.064). In recurrent miscarriages, a significant reduction in LXR mRNA expression compared to control placentas could be shown (P = 0.003) (Figure 2(b)). For quantification of LXR mRNA expression, mean values for LXRA and LXRB were used.

Bottom Line: LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy.In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α .To conclude, our data show that LXR expression is decreased in miscarriage.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-Universität München, 80337 Munich, Germany.

ABSTRACT
The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.

No MeSH data available.


Related in: MedlinePlus