Limits...
Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1.

Kluth SM, Radke TF, Kögler G - Stem Cells Int (2013)

Bottom Line: Multipotent stromal cells can be isolated from a variety of different tissues in the body.In this study, experiments assessing the haematopoiesis-supporting capacity and molecular biological analyses were conducted and clearly confirmed different properties.Compared to CB MSC, USSC lead to a higher expansion of haematopoietic cells and in addition express significantly higher levels of insulin-like growth factor binding protein 1 (IGFBP1), but lower levels of IGF2.

View Article: PubMed Central - PubMed

Affiliation: Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Medical Center, 40225 Duesseldorf, Germany.

ABSTRACT
Multipotent stromal cells can be isolated from a variety of different tissues in the body. In contrast to stromal cells from the adult bone marrow (BM) or adipose tissue, cord blood (CB) multipotent stromal cells (MSC) are biologically younger. Since first being described by our group, delta like 1 homologue (DLK-1) was determined as a discriminating factor between the distinct cord blood-derived subpopulations: the unrestricted somatic stromal cells (USSC), which lack adipogenic differentiation capacity, and the BM MSC-like CB MSC. In this study, experiments assessing the haematopoiesis-supporting capacity and molecular biological analyses were conducted and clearly confirmed different properties. Compared to CB MSC, USSC lead to a higher expansion of haematopoietic cells and in addition express significantly higher levels of insulin-like growth factor binding protein 1 (IGFBP1), but lower levels of IGF2. The data presented here also indicate that DLK-1 might not be the sole factor responsible for the inhibition of adipogenic differentiation potential in USSC but nevertheless indicates a biological diversity among cord blood-derived stromal cells.

No MeSH data available.


Related in: MedlinePlus

DLK-1 is a marker to distinguish USSC from CB MSC in cord blood. (a) Differentiation capacity of CB-derived stromal cells. Basal characterization of CB-derived stromal cells revealed the adipogenic differentiation as discriminating characteristic between CB MSC    (n = 29) and USSC (n = 61). CB MSC exhibited a high adipogenic differentiation potential, comparable to BM MSC, while USSC never formed lipid vacuoles following adipogenic induction as demonstrated by Oil Red O staining after 21 days of differentiation. (b) DLK-1 expression inversely correlated with the adipogenic differentiation potential. DLK-1 mRNA expression was significantly higher in USSC and clonal USSC lines compared to CB MSC and corresponding clonal populations. The DLK-1 expressing teratocarcinoma cell line nTERA-2 was tested as positive control, dermal fibroblasts (NHDF) served as biological negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3649714&req=5

fig1: DLK-1 is a marker to distinguish USSC from CB MSC in cord blood. (a) Differentiation capacity of CB-derived stromal cells. Basal characterization of CB-derived stromal cells revealed the adipogenic differentiation as discriminating characteristic between CB MSC    (n = 29) and USSC (n = 61). CB MSC exhibited a high adipogenic differentiation potential, comparable to BM MSC, while USSC never formed lipid vacuoles following adipogenic induction as demonstrated by Oil Red O staining after 21 days of differentiation. (b) DLK-1 expression inversely correlated with the adipogenic differentiation potential. DLK-1 mRNA expression was significantly higher in USSC and clonal USSC lines compared to CB MSC and corresponding clonal populations. The DLK-1 expressing teratocarcinoma cell line nTERA-2 was tested as positive control, dermal fibroblasts (NHDF) served as biological negative control.

Mentions: The presence of distinct stromal cell populations in cord blood (CB) has been described before [3, 4, 19]. Unrestricted somatic stromal cells (USSC) and CB multipotent stromal cells (MSC) share the same immunophenotype as well as osteogenic and chondrogenic differentiation potential in vitro. The two subpopulations can be classified by analyzing the adipogenic differentiation capacity and the HOX-gene expression profile. In contrast to CB MSC (n = 21) and MSC from bone marrow (BM MSC) (n = 44), USSC (n = 69) lack the adipogenic differentiation potential (Figure 1(a)) and as a general feature express high levels of DLK-1 on transcript level.


Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1.

Kluth SM, Radke TF, Kögler G - Stem Cells Int (2013)

DLK-1 is a marker to distinguish USSC from CB MSC in cord blood. (a) Differentiation capacity of CB-derived stromal cells. Basal characterization of CB-derived stromal cells revealed the adipogenic differentiation as discriminating characteristic between CB MSC    (n = 29) and USSC (n = 61). CB MSC exhibited a high adipogenic differentiation potential, comparable to BM MSC, while USSC never formed lipid vacuoles following adipogenic induction as demonstrated by Oil Red O staining after 21 days of differentiation. (b) DLK-1 expression inversely correlated with the adipogenic differentiation potential. DLK-1 mRNA expression was significantly higher in USSC and clonal USSC lines compared to CB MSC and corresponding clonal populations. The DLK-1 expressing teratocarcinoma cell line nTERA-2 was tested as positive control, dermal fibroblasts (NHDF) served as biological negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649714&req=5

fig1: DLK-1 is a marker to distinguish USSC from CB MSC in cord blood. (a) Differentiation capacity of CB-derived stromal cells. Basal characterization of CB-derived stromal cells revealed the adipogenic differentiation as discriminating characteristic between CB MSC    (n = 29) and USSC (n = 61). CB MSC exhibited a high adipogenic differentiation potential, comparable to BM MSC, while USSC never formed lipid vacuoles following adipogenic induction as demonstrated by Oil Red O staining after 21 days of differentiation. (b) DLK-1 expression inversely correlated with the adipogenic differentiation potential. DLK-1 mRNA expression was significantly higher in USSC and clonal USSC lines compared to CB MSC and corresponding clonal populations. The DLK-1 expressing teratocarcinoma cell line nTERA-2 was tested as positive control, dermal fibroblasts (NHDF) served as biological negative control.
Mentions: The presence of distinct stromal cell populations in cord blood (CB) has been described before [3, 4, 19]. Unrestricted somatic stromal cells (USSC) and CB multipotent stromal cells (MSC) share the same immunophenotype as well as osteogenic and chondrogenic differentiation potential in vitro. The two subpopulations can be classified by analyzing the adipogenic differentiation capacity and the HOX-gene expression profile. In contrast to CB MSC (n = 21) and MSC from bone marrow (BM MSC) (n = 44), USSC (n = 69) lack the adipogenic differentiation potential (Figure 1(a)) and as a general feature express high levels of DLK-1 on transcript level.

Bottom Line: Multipotent stromal cells can be isolated from a variety of different tissues in the body.In this study, experiments assessing the haematopoiesis-supporting capacity and molecular biological analyses were conducted and clearly confirmed different properties.Compared to CB MSC, USSC lead to a higher expansion of haematopoietic cells and in addition express significantly higher levels of insulin-like growth factor binding protein 1 (IGFBP1), but lower levels of IGF2.

View Article: PubMed Central - PubMed

Affiliation: Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Medical Center, 40225 Duesseldorf, Germany.

ABSTRACT
Multipotent stromal cells can be isolated from a variety of different tissues in the body. In contrast to stromal cells from the adult bone marrow (BM) or adipose tissue, cord blood (CB) multipotent stromal cells (MSC) are biologically younger. Since first being described by our group, delta like 1 homologue (DLK-1) was determined as a discriminating factor between the distinct cord blood-derived subpopulations: the unrestricted somatic stromal cells (USSC), which lack adipogenic differentiation capacity, and the BM MSC-like CB MSC. In this study, experiments assessing the haematopoiesis-supporting capacity and molecular biological analyses were conducted and clearly confirmed different properties. Compared to CB MSC, USSC lead to a higher expansion of haematopoietic cells and in addition express significantly higher levels of insulin-like growth factor binding protein 1 (IGFBP1), but lower levels of IGF2. The data presented here also indicate that DLK-1 might not be the sole factor responsible for the inhibition of adipogenic differentiation potential in USSC but nevertheless indicates a biological diversity among cord blood-derived stromal cells.

No MeSH data available.


Related in: MedlinePlus