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p38/AP-1 pathway in lipopolysaccharide-induced inflammatory responses is negatively modulated by electrical stimulation.

Jeong D, Lee J, Yi YS, Yang Y, Kim KW, Cho JY - Mediators Inflamm. (2013)

Bottom Line: Through immunoblotting and immunoprecipitation analyses, we found that p38/AP-1 could be the major inhibitory pathway in the NGACP-mediated anti-inflammatory response.Indeed, inhibition of p38 by SB203580 showed similar inhibitory activity of the production of TNF- α and PGE2 and the expression of TNF- α and COX-2 mRNA.These results suggest that ACP-induced nanocurrents alter signal transduction pathways that are involved in the inflammatory response and could therefore be utilized in the treatment of various inflammatory diseases such as arthritis and colitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.

ABSTRACT
Electrical stimulation with a weak current has been demonstrated to modulate various cellular and physiological responses, including the differentiation of mesenchymal stem cells and acute or chronic physical pain. Thus, a variety of investigations regarding the physiological role of nano- or microlevel currents at the cellular level are actively proceeding in the field of alternative medicine. In this study, we focused on the anti-inflammatory activity of aluminum-copper patches (ACPs) under macrophage-mediated inflammatory conditions. ACPs generated nanolevel currents ranging from 30 to 55 nA in solution conditions. Interestingly, the nanocurrent-generating aluminum-copper patches (NGACPs) were able to suppress both lipopolysaccharide-(LPS-) and pam3CSK-induced inflammatory responses such as NO and PGE2 production in both RAW264.7 cells and peritoneal macrophages at the transcriptional level. Through immunoblotting and immunoprecipitation analyses, we found that p38/AP-1 could be the major inhibitory pathway in the NGACP-mediated anti-inflammatory response. Indeed, inhibition of p38 by SB203580 showed similar inhibitory activity of the production of TNF- α and PGE2 and the expression of TNF- α and COX-2 mRNA. These results suggest that ACP-induced nanocurrents alter signal transduction pathways that are involved in the inflammatory response and could therefore be utilized in the treatment of various inflammatory diseases such as arthritis and colitis.

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Related in: MedlinePlus

Effect of nanocurrent-generating ACPs (NGACPs) treatment on the production of inflammatory mediators. ((a), (b), and (c)) The levels of NO and PGE2 were determined using a Griess assay and EIA with culture supernatants from RAW264.7 cells or peritoneal macrophages that had been treated with NGACP and LPS (1 μg/mL) or pam3CSK (10 μg/mL) for 6 or 24 h (NO and PGE2, resp.). (d) Morphological alterations in RAW264.7 cells and HEK293 cells were photographed using a digital camera. (e) The viability of RAW264.7 and HEK293 cells was determined using an MTT assay. *P < 0.05 and **P < 0.01 compared to control.
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fig2: Effect of nanocurrent-generating ACPs (NGACPs) treatment on the production of inflammatory mediators. ((a), (b), and (c)) The levels of NO and PGE2 were determined using a Griess assay and EIA with culture supernatants from RAW264.7 cells or peritoneal macrophages that had been treated with NGACP and LPS (1 μg/mL) or pam3CSK (10 μg/mL) for 6 or 24 h (NO and PGE2, resp.). (d) Morphological alterations in RAW264.7 cells and HEK293 cells were photographed using a digital camera. (e) The viability of RAW264.7 and HEK293 cells was determined using an MTT assay. *P < 0.05 and **P < 0.01 compared to control.

Mentions: Data (Figures 1, 2, and 4), expressed as the means ± standard deviation (SD), were calculated from one (n = 6) of two independent experiments. Other data are representative of three different experiments with similar results. For statistical comparisons, the results were analyzed using analysis of variance/Scheffe's post hoc test and the Kruskal-Wallis/Mann-Whitney test. All Pvalues < 0.05 were considered statistically significant. All of the statistical tests were carried out using the computer program SPSS (SPSS Inc., Chicago, IL, USA).


p38/AP-1 pathway in lipopolysaccharide-induced inflammatory responses is negatively modulated by electrical stimulation.

Jeong D, Lee J, Yi YS, Yang Y, Kim KW, Cho JY - Mediators Inflamm. (2013)

Effect of nanocurrent-generating ACPs (NGACPs) treatment on the production of inflammatory mediators. ((a), (b), and (c)) The levels of NO and PGE2 were determined using a Griess assay and EIA with culture supernatants from RAW264.7 cells or peritoneal macrophages that had been treated with NGACP and LPS (1 μg/mL) or pam3CSK (10 μg/mL) for 6 or 24 h (NO and PGE2, resp.). (d) Morphological alterations in RAW264.7 cells and HEK293 cells were photographed using a digital camera. (e) The viability of RAW264.7 and HEK293 cells was determined using an MTT assay. *P < 0.05 and **P < 0.01 compared to control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649710&req=5

fig2: Effect of nanocurrent-generating ACPs (NGACPs) treatment on the production of inflammatory mediators. ((a), (b), and (c)) The levels of NO and PGE2 were determined using a Griess assay and EIA with culture supernatants from RAW264.7 cells or peritoneal macrophages that had been treated with NGACP and LPS (1 μg/mL) or pam3CSK (10 μg/mL) for 6 or 24 h (NO and PGE2, resp.). (d) Morphological alterations in RAW264.7 cells and HEK293 cells were photographed using a digital camera. (e) The viability of RAW264.7 and HEK293 cells was determined using an MTT assay. *P < 0.05 and **P < 0.01 compared to control.
Mentions: Data (Figures 1, 2, and 4), expressed as the means ± standard deviation (SD), were calculated from one (n = 6) of two independent experiments. Other data are representative of three different experiments with similar results. For statistical comparisons, the results were analyzed using analysis of variance/Scheffe's post hoc test and the Kruskal-Wallis/Mann-Whitney test. All Pvalues < 0.05 were considered statistically significant. All of the statistical tests were carried out using the computer program SPSS (SPSS Inc., Chicago, IL, USA).

Bottom Line: Through immunoblotting and immunoprecipitation analyses, we found that p38/AP-1 could be the major inhibitory pathway in the NGACP-mediated anti-inflammatory response.Indeed, inhibition of p38 by SB203580 showed similar inhibitory activity of the production of TNF- α and PGE2 and the expression of TNF- α and COX-2 mRNA.These results suggest that ACP-induced nanocurrents alter signal transduction pathways that are involved in the inflammatory response and could therefore be utilized in the treatment of various inflammatory diseases such as arthritis and colitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.

ABSTRACT
Electrical stimulation with a weak current has been demonstrated to modulate various cellular and physiological responses, including the differentiation of mesenchymal stem cells and acute or chronic physical pain. Thus, a variety of investigations regarding the physiological role of nano- or microlevel currents at the cellular level are actively proceeding in the field of alternative medicine. In this study, we focused on the anti-inflammatory activity of aluminum-copper patches (ACPs) under macrophage-mediated inflammatory conditions. ACPs generated nanolevel currents ranging from 30 to 55 nA in solution conditions. Interestingly, the nanocurrent-generating aluminum-copper patches (NGACPs) were able to suppress both lipopolysaccharide-(LPS-) and pam3CSK-induced inflammatory responses such as NO and PGE2 production in both RAW264.7 cells and peritoneal macrophages at the transcriptional level. Through immunoblotting and immunoprecipitation analyses, we found that p38/AP-1 could be the major inhibitory pathway in the NGACP-mediated anti-inflammatory response. Indeed, inhibition of p38 by SB203580 showed similar inhibitory activity of the production of TNF- α and PGE2 and the expression of TNF- α and COX-2 mRNA. These results suggest that ACP-induced nanocurrents alter signal transduction pathways that are involved in the inflammatory response and could therefore be utilized in the treatment of various inflammatory diseases such as arthritis and colitis.

Show MeSH
Related in: MedlinePlus