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A qualitative survey of five antibiotics in a water treatment plant in central plateau of Iran.

Heidari M, Kazemipour M, Bina B, Ebrahimi A, Ansari M, Ghasemian M, Amin MM - J Environ Public Health (2013)

Bottom Line: Also two sampling techniques, passive and grab samplings, were compared in the detection of selected antibiotics.The results showed that enrofloxacin, oxytetracycline, and tylosin were not detected in none of the samples.The results imply that passive sampling is a better approach than grab sampling for the investigation of antibiotics in aquatic environments.

View Article: PubMed Central - PubMed

Affiliation: Environment Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Introduction: This study aimed to survey a total of five common human and veterinary antibiotics based on SPE-LC-MS-MS technology in a water treatment plant at central plateau of Iran. Also two sampling techniques, passive and grab samplings, were compared in the detection of selected antibiotics.

Materials and methods: In January to March 2012, grab and passive samples were taken from the influent and effluent of a water treatment plant. The samples were prepared using solid-phase extraction (SPE), and extracts were analyzed by liquid chromatography tandem mass spectrometry (LC-MS-MS).

Results: The results showed that enrofloxacin, oxytetracycline, and tylosin were not detected in none of the samples. However, ampicillin was detected in the grab and passive samples taken from the influent (source water) of the plant, and ciprofloxacin was detected in passive samples taken from the influent and effluent (finished water) of the plant.

Conclusion: The results imply that passive sampling is a better approach than grab sampling for the investigation of antibiotics in aquatic environments. The presence of ampicillin and ciprofloxacin in source water and finished water of the water treatment plant may lead to potential emergence of resistant bacteria that should be considered in future studies.

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Schematic of SPE set up.
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Related In: Results  -  Collection


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fig3: Schematic of SPE set up.

Mentions: Solid-phase extraction (SPE) experiments were conducted using 200 mg/6 mL Oasis HLB cartridges on an innovative setup (Figure 3). The cartridges were preconditioned with 4 mL of MeOH and 6 mL of deionized water. A volume of 1000 mL of water sample with pH 2.8–3 (H2SO4) was passed through the cartridge at a flow-rate of 5–8 mL min−1 using a vacuum extraction manifold at 7–9 in.Hg (Visiprepä, Supelco, Bellefonte, PA, USA; 1 in.Hg = 338.638 Pa). Afterwards the cartridges were rinsed with 10 mL of ultra-pure water and were air-dried for 5 min. The retained analytes were subsequently eluted with 10 mL of methanol into a glass test tube. The extract was concentrated to dryness under a stream of N and reconstituted to ~250 μL in a solvent mixture of ultra-pure water/methanol (9 : 1). The extract was filtered through a 4 mm i.d., 0.2 μm pore size cellulose acetate syringe filters, transferred to an amber vial, and stored at −15°C until LC-MS/MS analysis.


A qualitative survey of five antibiotics in a water treatment plant in central plateau of Iran.

Heidari M, Kazemipour M, Bina B, Ebrahimi A, Ansari M, Ghasemian M, Amin MM - J Environ Public Health (2013)

Schematic of SPE set up.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3649666&req=5

fig3: Schematic of SPE set up.
Mentions: Solid-phase extraction (SPE) experiments were conducted using 200 mg/6 mL Oasis HLB cartridges on an innovative setup (Figure 3). The cartridges were preconditioned with 4 mL of MeOH and 6 mL of deionized water. A volume of 1000 mL of water sample with pH 2.8–3 (H2SO4) was passed through the cartridge at a flow-rate of 5–8 mL min−1 using a vacuum extraction manifold at 7–9 in.Hg (Visiprepä, Supelco, Bellefonte, PA, USA; 1 in.Hg = 338.638 Pa). Afterwards the cartridges were rinsed with 10 mL of ultra-pure water and were air-dried for 5 min. The retained analytes were subsequently eluted with 10 mL of methanol into a glass test tube. The extract was concentrated to dryness under a stream of N and reconstituted to ~250 μL in a solvent mixture of ultra-pure water/methanol (9 : 1). The extract was filtered through a 4 mm i.d., 0.2 μm pore size cellulose acetate syringe filters, transferred to an amber vial, and stored at −15°C until LC-MS/MS analysis.

Bottom Line: Also two sampling techniques, passive and grab samplings, were compared in the detection of selected antibiotics.The results showed that enrofloxacin, oxytetracycline, and tylosin were not detected in none of the samples.The results imply that passive sampling is a better approach than grab sampling for the investigation of antibiotics in aquatic environments.

View Article: PubMed Central - PubMed

Affiliation: Environment Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Introduction: This study aimed to survey a total of five common human and veterinary antibiotics based on SPE-LC-MS-MS technology in a water treatment plant at central plateau of Iran. Also two sampling techniques, passive and grab samplings, were compared in the detection of selected antibiotics.

Materials and methods: In January to March 2012, grab and passive samples were taken from the influent and effluent of a water treatment plant. The samples were prepared using solid-phase extraction (SPE), and extracts were analyzed by liquid chromatography tandem mass spectrometry (LC-MS-MS).

Results: The results showed that enrofloxacin, oxytetracycline, and tylosin were not detected in none of the samples. However, ampicillin was detected in the grab and passive samples taken from the influent (source water) of the plant, and ciprofloxacin was detected in passive samples taken from the influent and effluent (finished water) of the plant.

Conclusion: The results imply that passive sampling is a better approach than grab sampling for the investigation of antibiotics in aquatic environments. The presence of ampicillin and ciprofloxacin in source water and finished water of the water treatment plant may lead to potential emergence of resistant bacteria that should be considered in future studies.

Show MeSH