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The overnight effect of dietary energy balance on postprandial plasma free amino acid (PFAA) profiles in Japanese adult men.

Nishioka M, Imaizumi A, Ando T, Tochikubo O - PLoS ONE (2013)

Bottom Line: We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal.Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds.If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

View Article: PubMed Central - PubMed

Affiliation: Liberty Square Clinic, Comfort Medical Foundation, Kawasaki, Kanagawa, Japan.

ABSTRACT
The plasma free amino acid (PFAA) profile is affected by various nutritional conditions, such as the dietary energy balance. Regarding the clinical use of PFAA profiling, it is of concern that differences in food ingestion patterns may generate systematic errors in a plasma amino acid profile and constitute a confounding factor in assessment. In this study, the overnight impact of the dietary energy balance on the postprandial plasma amino acid profile was investigated to elucidate in particular the effects of high protein meals typical in Japanese cuisine. We conducted diet-controlled, crossover trials in eleven healthy male volunteers aged 40-61 y. They consumed either a normal meal (meal N) or high protein meal (meal H) at dinner. Forearm venous blood was collected, and plasma amino acid concentrations were measured before dinner and the next morning. We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal. Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds. If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

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Related in: MedlinePlus

The relationship between the amino acid composition of chicken and the increase of the average plasma amino acid concentration observed in meal H-ingested subjects at 6∶00 am.Black symbols indicate non-essential amino acids (NEAAs), and red symbols indicate essential amino acids. The line indicates the linear regression line for all amino acids.
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pone-0062929-g003: The relationship between the amino acid composition of chicken and the increase of the average plasma amino acid concentration observed in meal H-ingested subjects at 6∶00 am.Black symbols indicate non-essential amino acids (NEAAs), and red symbols indicate essential amino acids. The line indicates the linear regression line for all amino acids.

Mentions: Next, the balance between the amount of ingested amino acids and the PFAA concentrations was investigated. As described in Subjects and Methods, the most dominant part of the protein ingestion was attributed to the increase in the amount of chicken for high protein meal-ingested subjects. Therefore, the amino acid composition of chicken (http://www.mext.go.jp/b_menu/shingi/gijyutu/gijyutu3/houkoku/1298881.htm) may reflect the uptake of each amino acid. However, there was only a poor correlation between the increase in ingested amino acids and PFAA concentrations (Fig. 3). The non-essential amino acids Ala, Gly, Asp+Asn, and Glu+Gln showed an increase in plasma levels that was apparently repressed in comparison to the increase in ingestion. Inversely, in the case of BCAAs and Lys, a relatively higher increase in plasma levels was observed in comparison to the increase of ingestion (Fig. 3). The same tendency was observed at 8∶00 am and 10∶00 am (data not shown).


The overnight effect of dietary energy balance on postprandial plasma free amino acid (PFAA) profiles in Japanese adult men.

Nishioka M, Imaizumi A, Ando T, Tochikubo O - PLoS ONE (2013)

The relationship between the amino acid composition of chicken and the increase of the average plasma amino acid concentration observed in meal H-ingested subjects at 6∶00 am.Black symbols indicate non-essential amino acids (NEAAs), and red symbols indicate essential amino acids. The line indicates the linear regression line for all amino acids.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646894&req=5

pone-0062929-g003: The relationship between the amino acid composition of chicken and the increase of the average plasma amino acid concentration observed in meal H-ingested subjects at 6∶00 am.Black symbols indicate non-essential amino acids (NEAAs), and red symbols indicate essential amino acids. The line indicates the linear regression line for all amino acids.
Mentions: Next, the balance between the amount of ingested amino acids and the PFAA concentrations was investigated. As described in Subjects and Methods, the most dominant part of the protein ingestion was attributed to the increase in the amount of chicken for high protein meal-ingested subjects. Therefore, the amino acid composition of chicken (http://www.mext.go.jp/b_menu/shingi/gijyutu/gijyutu3/houkoku/1298881.htm) may reflect the uptake of each amino acid. However, there was only a poor correlation between the increase in ingested amino acids and PFAA concentrations (Fig. 3). The non-essential amino acids Ala, Gly, Asp+Asn, and Glu+Gln showed an increase in plasma levels that was apparently repressed in comparison to the increase in ingestion. Inversely, in the case of BCAAs and Lys, a relatively higher increase in plasma levels was observed in comparison to the increase of ingestion (Fig. 3). The same tendency was observed at 8∶00 am and 10∶00 am (data not shown).

Bottom Line: We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal.Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds.If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

View Article: PubMed Central - PubMed

Affiliation: Liberty Square Clinic, Comfort Medical Foundation, Kawasaki, Kanagawa, Japan.

ABSTRACT
The plasma free amino acid (PFAA) profile is affected by various nutritional conditions, such as the dietary energy balance. Regarding the clinical use of PFAA profiling, it is of concern that differences in food ingestion patterns may generate systematic errors in a plasma amino acid profile and constitute a confounding factor in assessment. In this study, the overnight impact of the dietary energy balance on the postprandial plasma amino acid profile was investigated to elucidate in particular the effects of high protein meals typical in Japanese cuisine. We conducted diet-controlled, crossover trials in eleven healthy male volunteers aged 40-61 y. They consumed either a normal meal (meal N) or high protein meal (meal H) at dinner. Forearm venous blood was collected, and plasma amino acid concentrations were measured before dinner and the next morning. We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal. Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds. If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

Show MeSH
Related in: MedlinePlus