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The overnight effect of dietary energy balance on postprandial plasma free amino acid (PFAA) profiles in Japanese adult men.

Nishioka M, Imaizumi A, Ando T, Tochikubo O - PLoS ONE (2013)

Bottom Line: We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal.Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds.If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

View Article: PubMed Central - PubMed

Affiliation: Liberty Square Clinic, Comfort Medical Foundation, Kawasaki, Kanagawa, Japan.

ABSTRACT
The plasma free amino acid (PFAA) profile is affected by various nutritional conditions, such as the dietary energy balance. Regarding the clinical use of PFAA profiling, it is of concern that differences in food ingestion patterns may generate systematic errors in a plasma amino acid profile and constitute a confounding factor in assessment. In this study, the overnight impact of the dietary energy balance on the postprandial plasma amino acid profile was investigated to elucidate in particular the effects of high protein meals typical in Japanese cuisine. We conducted diet-controlled, crossover trials in eleven healthy male volunteers aged 40-61 y. They consumed either a normal meal (meal N) or high protein meal (meal H) at dinner. Forearm venous blood was collected, and plasma amino acid concentrations were measured before dinner and the next morning. We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal. Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds. If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

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Related in: MedlinePlus

Preprandial and postprandial trends of the PFAA-based indices of meal N-ingested subjects (blue lines) and meal H-ingested subjects (red lines).*: Significant at p<0.05 with the Bonferroni multicomparison test after 2-way ANOVA. **: Significant at p<0.01 with the Bonferroni multicomparison test after 2-way ANOVA. ***: Significant at p<0.001 with the Bonferroni multicomparison test after 2-way ANOVA.
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pone-0062929-g002: Preprandial and postprandial trends of the PFAA-based indices of meal N-ingested subjects (blue lines) and meal H-ingested subjects (red lines).*: Significant at p<0.05 with the Bonferroni multicomparison test after 2-way ANOVA. **: Significant at p<0.01 with the Bonferroni multicomparison test after 2-way ANOVA. ***: Significant at p<0.001 with the Bonferroni multicomparison test after 2-way ANOVA.

Mentions: In other cases, in particular for most of the essential amino acids, imbalances between the increase of amino acid ingestion originating from dietary protein and plasma levels were still observed at 8, 10, and 12 hours after ingestion. For example, apparent imbalances in the neutral amino acids were observed. The plasma Fischer ratio in meal H-ingested subjects was higher than that of meal N-ingested subjects (Fig. 2A), and this is partially attributed to the fact that the BCAAs were not catabolized in the liver [26], [33]. Inversely, plasma Trp per sum of large neutral amino acids (LNAAs; Val, Ile, Leu, Tyr, and Phe) and Tyr/LNAAs were decreased in meal H-ingested subjects (Fig. 2B and Fig. 2C). Furthermore, scores of lung cancer classifier, which was the multiple logistic regression function composed of six amino acids (Ala, Val, Ile, His, Trp, and Orn) were significantly elevated (i.e. higher probability of lung cancer) in meal H-ingested subjects (Fig. 2D). Therefore, it was demonstrated that overnight postprandial PFAA profiles after ingestion of meal H-ingestion would result wrong decision in terms of health condition even in normal subjects.


The overnight effect of dietary energy balance on postprandial plasma free amino acid (PFAA) profiles in Japanese adult men.

Nishioka M, Imaizumi A, Ando T, Tochikubo O - PLoS ONE (2013)

Preprandial and postprandial trends of the PFAA-based indices of meal N-ingested subjects (blue lines) and meal H-ingested subjects (red lines).*: Significant at p<0.05 with the Bonferroni multicomparison test after 2-way ANOVA. **: Significant at p<0.01 with the Bonferroni multicomparison test after 2-way ANOVA. ***: Significant at p<0.001 with the Bonferroni multicomparison test after 2-way ANOVA.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646894&req=5

pone-0062929-g002: Preprandial and postprandial trends of the PFAA-based indices of meal N-ingested subjects (blue lines) and meal H-ingested subjects (red lines).*: Significant at p<0.05 with the Bonferroni multicomparison test after 2-way ANOVA. **: Significant at p<0.01 with the Bonferroni multicomparison test after 2-way ANOVA. ***: Significant at p<0.001 with the Bonferroni multicomparison test after 2-way ANOVA.
Mentions: In other cases, in particular for most of the essential amino acids, imbalances between the increase of amino acid ingestion originating from dietary protein and plasma levels were still observed at 8, 10, and 12 hours after ingestion. For example, apparent imbalances in the neutral amino acids were observed. The plasma Fischer ratio in meal H-ingested subjects was higher than that of meal N-ingested subjects (Fig. 2A), and this is partially attributed to the fact that the BCAAs were not catabolized in the liver [26], [33]. Inversely, plasma Trp per sum of large neutral amino acids (LNAAs; Val, Ile, Leu, Tyr, and Phe) and Tyr/LNAAs were decreased in meal H-ingested subjects (Fig. 2B and Fig. 2C). Furthermore, scores of lung cancer classifier, which was the multiple logistic regression function composed of six amino acids (Ala, Val, Ile, His, Trp, and Orn) were significantly elevated (i.e. higher probability of lung cancer) in meal H-ingested subjects (Fig. 2D). Therefore, it was demonstrated that overnight postprandial PFAA profiles after ingestion of meal H-ingestion would result wrong decision in terms of health condition even in normal subjects.

Bottom Line: We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal.Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds.If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

View Article: PubMed Central - PubMed

Affiliation: Liberty Square Clinic, Comfort Medical Foundation, Kawasaki, Kanagawa, Japan.

ABSTRACT
The plasma free amino acid (PFAA) profile is affected by various nutritional conditions, such as the dietary energy balance. Regarding the clinical use of PFAA profiling, it is of concern that differences in food ingestion patterns may generate systematic errors in a plasma amino acid profile and constitute a confounding factor in assessment. In this study, the overnight impact of the dietary energy balance on the postprandial plasma amino acid profile was investigated to elucidate in particular the effects of high protein meals typical in Japanese cuisine. We conducted diet-controlled, crossover trials in eleven healthy male volunteers aged 40-61 y. They consumed either a normal meal (meal N) or high protein meal (meal H) at dinner. Forearm venous blood was collected, and plasma amino acid concentrations were measured before dinner and the next morning. We found that a high protein meal in the evening that contained 40% energy would significantly increase the PFAA concentration the next morning, even more than 12 hours after the meal. Among amino acids, the most significant difference was observed in the branched-chain amino acids (BCAAs) and in some urea-cycle related compounds. If the subject consumed the high protein diet at dinner, the PFAA profile after overnight fasting might be still affected by the meal even 12 hours after the meal, suggesting that the PFAA profile does not reflect the subject's health condition, but rather the acute effect of high protein ingestion.

Show MeSH
Related in: MedlinePlus