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Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.

Roperto S, Russo V, Ozkul A, Corteggio A, Sepici-Dincel A, Catoi C, Esposito I, Riccardi MG, Urraro C, Lucà R, Ceccarelli DM, Longo M, Roperto F - PLoS ONE (2013)

Bottom Line: L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA.Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium.Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Sezione Malattie Infettive, Università di Napoli Federico II, Naples, Italy. sante.roperto@unina.it

ABSTRACT

Background: Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors.

Methods and findings: E5 protein, the major oncoprotein of the BPV-2, was detected in all tumors. L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium. Finally, the early protein E2, required for viral DNA replication and known to be a pivotal factor for both productive and persistent infection, was detected by Western blot and immunohistochemically. Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

Conclusion: This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo.

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Related in: MedlinePlus

Presence of viral particles in bovine urothelial cells.A) Electron microscopical examination showed numerous intranuclear electron dense particles, 45–50 nm in diameter (black arrows). X 15,000. The size and shape of these particles are consistent with the submicroscopic features of viral particles (insert). X 50,000. B) Normal urothelial cells. No electron dense particles are detected in the nucleus of a normal urothelial cell. X 15,000.
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pone-0062227-g007: Presence of viral particles in bovine urothelial cells.A) Electron microscopical examination showed numerous intranuclear electron dense particles, 45–50 nm in diameter (black arrows). X 15,000. The size and shape of these particles are consistent with the submicroscopic features of viral particles (insert). X 50,000. B) Normal urothelial cells. No electron dense particles are detected in the nucleus of a normal urothelial cell. X 15,000.

Mentions: Ultrastructural studies showed electron dense particles, about 50 nm in diameter, the size and shape of which were consistent with the submicroscopic features of viral particles both in the nuclei of bovine and bubaline urothelial cells (Figure 7A and 8A). Similar electron dense particles were not observed in the nuclei of urothelial cells from urinary bladders of healthy cows and buffaloes (Figure 7B and 8B).


Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.

Roperto S, Russo V, Ozkul A, Corteggio A, Sepici-Dincel A, Catoi C, Esposito I, Riccardi MG, Urraro C, Lucà R, Ceccarelli DM, Longo M, Roperto F - PLoS ONE (2013)

Presence of viral particles in bovine urothelial cells.A) Electron microscopical examination showed numerous intranuclear electron dense particles, 45–50 nm in diameter (black arrows). X 15,000. The size and shape of these particles are consistent with the submicroscopic features of viral particles (insert). X 50,000. B) Normal urothelial cells. No electron dense particles are detected in the nucleus of a normal urothelial cell. X 15,000.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646877&req=5

pone-0062227-g007: Presence of viral particles in bovine urothelial cells.A) Electron microscopical examination showed numerous intranuclear electron dense particles, 45–50 nm in diameter (black arrows). X 15,000. The size and shape of these particles are consistent with the submicroscopic features of viral particles (insert). X 50,000. B) Normal urothelial cells. No electron dense particles are detected in the nucleus of a normal urothelial cell. X 15,000.
Mentions: Ultrastructural studies showed electron dense particles, about 50 nm in diameter, the size and shape of which were consistent with the submicroscopic features of viral particles both in the nuclei of bovine and bubaline urothelial cells (Figure 7A and 8A). Similar electron dense particles were not observed in the nuclei of urothelial cells from urinary bladders of healthy cows and buffaloes (Figure 7B and 8B).

Bottom Line: L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA.Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium.Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Sezione Malattie Infettive, Università di Napoli Federico II, Naples, Italy. sante.roperto@unina.it

ABSTRACT

Background: Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors.

Methods and findings: E5 protein, the major oncoprotein of the BPV-2, was detected in all tumors. L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium. Finally, the early protein E2, required for viral DNA replication and known to be a pivotal factor for both productive and persistent infection, was detected by Western blot and immunohistochemically. Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

Conclusion: This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo.

Show MeSH
Related in: MedlinePlus