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Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.

Roperto S, Russo V, Ozkul A, Corteggio A, Sepici-Dincel A, Catoi C, Esposito I, Riccardi MG, Urraro C, Lucà R, Ceccarelli DM, Longo M, Roperto F - PLoS ONE (2013)

Bottom Line: L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA.Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium.Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Sezione Malattie Infettive, Università di Napoli Federico II, Naples, Italy. sante.roperto@unina.it

ABSTRACT

Background: Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors.

Methods and findings: E5 protein, the major oncoprotein of the BPV-2, was detected in all tumors. L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium. Finally, the early protein E2, required for viral DNA replication and known to be a pivotal factor for both productive and persistent infection, was detected by Western blot and immunohistochemically. Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

Conclusion: This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo.

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Related in: MedlinePlus

L1 immunohistochemistry.A) Immunohistochemical evidence of L1 protein in urothelial neoplastic cells. B) No L1 protein immunoreactivity was detected in normal urothelial cells.
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pone-0062227-g004: L1 immunohistochemistry.A) Immunohistochemical evidence of L1 protein in urothelial neoplastic cells. B) No L1 protein immunoreactivity was detected in normal urothelial cells.

Mentions: Next, we assessed whether the L1 gene was expressed. We studied the expression of L1 across the spectrum of urothelial neoplastic lesions as it is well known that L1 expression reflects type and grade of tumors [23]. The presence of L1 protein in urothelial tumors of both cows and buffaloes was revealed by Western blot analysis (Figure 3). Its presence was confirmed morphologically. Immunohistochemically, a marked immunoreactivity was evident both in the cytoplasm and nuclei of the urothelial cells of neoplastic nests. Surprisingly, L1 protein expression was also evident in the basal neoplastic urothelial cells lining microcysts containing pale pink secretions (Figure 4A). No L1 protein expression was detected in normal urothelial cells (Figure 4B).


Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.

Roperto S, Russo V, Ozkul A, Corteggio A, Sepici-Dincel A, Catoi C, Esposito I, Riccardi MG, Urraro C, Lucà R, Ceccarelli DM, Longo M, Roperto F - PLoS ONE (2013)

L1 immunohistochemistry.A) Immunohistochemical evidence of L1 protein in urothelial neoplastic cells. B) No L1 protein immunoreactivity was detected in normal urothelial cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646877&req=5

pone-0062227-g004: L1 immunohistochemistry.A) Immunohistochemical evidence of L1 protein in urothelial neoplastic cells. B) No L1 protein immunoreactivity was detected in normal urothelial cells.
Mentions: Next, we assessed whether the L1 gene was expressed. We studied the expression of L1 across the spectrum of urothelial neoplastic lesions as it is well known that L1 expression reflects type and grade of tumors [23]. The presence of L1 protein in urothelial tumors of both cows and buffaloes was revealed by Western blot analysis (Figure 3). Its presence was confirmed morphologically. Immunohistochemically, a marked immunoreactivity was evident both in the cytoplasm and nuclei of the urothelial cells of neoplastic nests. Surprisingly, L1 protein expression was also evident in the basal neoplastic urothelial cells lining microcysts containing pale pink secretions (Figure 4A). No L1 protein expression was detected in normal urothelial cells (Figure 4B).

Bottom Line: L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA.Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium.Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Sezione Malattie Infettive, Università di Napoli Federico II, Naples, Italy. sante.roperto@unina.it

ABSTRACT

Background: Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors.

Methods and findings: E5 protein, the major oncoprotein of the BPV-2, was detected in all tumors. L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium. Finally, the early protein E2, required for viral DNA replication and known to be a pivotal factor for both productive and persistent infection, was detected by Western blot and immunohistochemically. Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.

Conclusion: This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo.

Show MeSH
Related in: MedlinePlus