Limits...
Expression patterns of GmAP2/EREB-like transcription factors involved in soybean responses to water deficit.

Marcolino-Gomes J, Rodrigues FA, Oliveira MC, Farias JR, Neumaier N, Abdelnoor RV, Marcelino-GuimarĂ£es FC, Nepomuceno AL - PLoS ONE (2013)

Bottom Line: We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator.The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress.Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, State University of Londrina, Londrina, Brazil.

ABSTRACT
Soybean farming has faced several losses in productivity due to drought events in the last few decades. However, plants have molecular mechanisms to prevent and protect against water deficit injuries, and transcription factors play an important role in triggering different defense mechanisms. Understanding the expression patterns of transcription factors in response to water deficit and to environmental diurnal changes is very important for unveiling water deficit stress tolerance mechanisms. Here, we analyzed the expression patterns of ten APETALA2/Ethylene Responsive Element Binding-like (AP2/EREB-like) transcription factors in two soybean genotypes (BR16: drought-sensitive; and Embrapa 48: drought-tolerant). According to phylogenetic and domain analyses, these genes can be included in the DREB and ERF subfamilies. We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator. We detected the up-regulation of 9 GmAP2/EREB-like genes and identified transcriptional differences that were dependent on the levels of the stress applied and the tissue type analyzed (the expression of the GmDREB1F-like gene, for example, was four times higher in roots than in leaves). The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress. Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

Show MeSH

Related in: MedlinePlus

RNAseq data of the AP2/EREB and DRIP2-like genes.Gene expression was measured in leaf tissues of BR 16 soybeans subjected to water deficit (drought) and normal hydration conditions (control). Gene expression (Exp. Log. [log2]) was evaluated over a 24-h time course from the time the light came on (ZT0) in 4-h intervals. Error bars represent the standard error (SEM).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3646874&req=5

pone-0062294-g005: RNAseq data of the AP2/EREB and DRIP2-like genes.Gene expression was measured in leaf tissues of BR 16 soybeans subjected to water deficit (drought) and normal hydration conditions (control). Gene expression (Exp. Log. [log2]) was evaluated over a 24-h time course from the time the light came on (ZT0) in 4-h intervals. Error bars represent the standard error (SEM).

Mentions: To observe if the target genes oscillated in their expression patterns over the course of the day, we used the RNAseq reads from the BR16 genotype, which were mapped in the soybean genome. Under control conditions, the expression of the DREB-related genes generally increased just before dawn and reached their peak expression between 8 and 12 h (Fig. 5). Interestingly, the stress induction of these genes also displayed a strong oscillation over the course of the day. Based on the gene expression patterns in response to water deficit, the genes we analyzed could be divided into three groups: group A was characterized by high expression levels during the end of the night and the beginning of the day, with lowest expression levels in the afternoon (16 h); group B corresponded to genes with higher expression levels in the beginning of the day and low expression levels at the end of the day; group C was represented by genes significantly induced by water deficit stress approximately 2 h before lights went out, at 20 h.


Expression patterns of GmAP2/EREB-like transcription factors involved in soybean responses to water deficit.

Marcolino-Gomes J, Rodrigues FA, Oliveira MC, Farias JR, Neumaier N, Abdelnoor RV, Marcelino-GuimarĂ£es FC, Nepomuceno AL - PLoS ONE (2013)

RNAseq data of the AP2/EREB and DRIP2-like genes.Gene expression was measured in leaf tissues of BR 16 soybeans subjected to water deficit (drought) and normal hydration conditions (control). Gene expression (Exp. Log. [log2]) was evaluated over a 24-h time course from the time the light came on (ZT0) in 4-h intervals. Error bars represent the standard error (SEM).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646874&req=5

pone-0062294-g005: RNAseq data of the AP2/EREB and DRIP2-like genes.Gene expression was measured in leaf tissues of BR 16 soybeans subjected to water deficit (drought) and normal hydration conditions (control). Gene expression (Exp. Log. [log2]) was evaluated over a 24-h time course from the time the light came on (ZT0) in 4-h intervals. Error bars represent the standard error (SEM).
Mentions: To observe if the target genes oscillated in their expression patterns over the course of the day, we used the RNAseq reads from the BR16 genotype, which were mapped in the soybean genome. Under control conditions, the expression of the DREB-related genes generally increased just before dawn and reached their peak expression between 8 and 12 h (Fig. 5). Interestingly, the stress induction of these genes also displayed a strong oscillation over the course of the day. Based on the gene expression patterns in response to water deficit, the genes we analyzed could be divided into three groups: group A was characterized by high expression levels during the end of the night and the beginning of the day, with lowest expression levels in the afternoon (16 h); group B corresponded to genes with higher expression levels in the beginning of the day and low expression levels at the end of the day; group C was represented by genes significantly induced by water deficit stress approximately 2 h before lights went out, at 20 h.

Bottom Line: We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator.The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress.Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, State University of Londrina, Londrina, Brazil.

ABSTRACT
Soybean farming has faced several losses in productivity due to drought events in the last few decades. However, plants have molecular mechanisms to prevent and protect against water deficit injuries, and transcription factors play an important role in triggering different defense mechanisms. Understanding the expression patterns of transcription factors in response to water deficit and to environmental diurnal changes is very important for unveiling water deficit stress tolerance mechanisms. Here, we analyzed the expression patterns of ten APETALA2/Ethylene Responsive Element Binding-like (AP2/EREB-like) transcription factors in two soybean genotypes (BR16: drought-sensitive; and Embrapa 48: drought-tolerant). According to phylogenetic and domain analyses, these genes can be included in the DREB and ERF subfamilies. We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator. We detected the up-regulation of 9 GmAP2/EREB-like genes and identified transcriptional differences that were dependent on the levels of the stress applied and the tissue type analyzed (the expression of the GmDREB1F-like gene, for example, was four times higher in roots than in leaves). The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress. Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

Show MeSH
Related in: MedlinePlus