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Expression patterns of GmAP2/EREB-like transcription factors involved in soybean responses to water deficit.

Marcolino-Gomes J, Rodrigues FA, Oliveira MC, Farias JR, Neumaier N, Abdelnoor RV, Marcelino-Guimarães FC, Nepomuceno AL - PLoS ONE (2013)

Bottom Line: We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator.The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress.Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, State University of Londrina, Londrina, Brazil.

ABSTRACT
Soybean farming has faced several losses in productivity due to drought events in the last few decades. However, plants have molecular mechanisms to prevent and protect against water deficit injuries, and transcription factors play an important role in triggering different defense mechanisms. Understanding the expression patterns of transcription factors in response to water deficit and to environmental diurnal changes is very important for unveiling water deficit stress tolerance mechanisms. Here, we analyzed the expression patterns of ten APETALA2/Ethylene Responsive Element Binding-like (AP2/EREB-like) transcription factors in two soybean genotypes (BR16: drought-sensitive; and Embrapa 48: drought-tolerant). According to phylogenetic and domain analyses, these genes can be included in the DREB and ERF subfamilies. We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator. We detected the up-regulation of 9 GmAP2/EREB-like genes and identified transcriptional differences that were dependent on the levels of the stress applied and the tissue type analyzed (the expression of the GmDREB1F-like gene, for example, was four times higher in roots than in leaves). The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress. Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

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Related in: MedlinePlus

Stability analyses of endogenous genes.In total, six candidate genes were evaluated using the NormFinder and GeNorm programs to select the most stable genes. The Y axis represents the Expression Stability Measure (M) from the GeNorm program and the Stability value from the NormFinder program. Genes are ranked from less stable (higher values) to most stable (genes with lower values).
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pone-0062294-g003: Stability analyses of endogenous genes.In total, six candidate genes were evaluated using the NormFinder and GeNorm programs to select the most stable genes. The Y axis represents the Expression Stability Measure (M) from the GeNorm program and the Stability value from the NormFinder program. Genes are ranked from less stable (higher values) to most stable (genes with lower values).

Mentions: Based on results obtained using the NormFinder software package, the Elongation Factor 1β (ELF-1β) and β-actin genes were the most stably expressed in the leaf samples, whereas in the roots, the α-tubulin, ELF-1β and β-actin genes were also significantly stable (Figs. 3A and B).


Expression patterns of GmAP2/EREB-like transcription factors involved in soybean responses to water deficit.

Marcolino-Gomes J, Rodrigues FA, Oliveira MC, Farias JR, Neumaier N, Abdelnoor RV, Marcelino-Guimarães FC, Nepomuceno AL - PLoS ONE (2013)

Stability analyses of endogenous genes.In total, six candidate genes were evaluated using the NormFinder and GeNorm programs to select the most stable genes. The Y axis represents the Expression Stability Measure (M) from the GeNorm program and the Stability value from the NormFinder program. Genes are ranked from less stable (higher values) to most stable (genes with lower values).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646874&req=5

pone-0062294-g003: Stability analyses of endogenous genes.In total, six candidate genes were evaluated using the NormFinder and GeNorm programs to select the most stable genes. The Y axis represents the Expression Stability Measure (M) from the GeNorm program and the Stability value from the NormFinder program. Genes are ranked from less stable (higher values) to most stable (genes with lower values).
Mentions: Based on results obtained using the NormFinder software package, the Elongation Factor 1β (ELF-1β) and β-actin genes were the most stably expressed in the leaf samples, whereas in the roots, the α-tubulin, ELF-1β and β-actin genes were also significantly stable (Figs. 3A and B).

Bottom Line: We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator.The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress.Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, State University of Londrina, Londrina, Brazil.

ABSTRACT
Soybean farming has faced several losses in productivity due to drought events in the last few decades. However, plants have molecular mechanisms to prevent and protect against water deficit injuries, and transcription factors play an important role in triggering different defense mechanisms. Understanding the expression patterns of transcription factors in response to water deficit and to environmental diurnal changes is very important for unveiling water deficit stress tolerance mechanisms. Here, we analyzed the expression patterns of ten APETALA2/Ethylene Responsive Element Binding-like (AP2/EREB-like) transcription factors in two soybean genotypes (BR16: drought-sensitive; and Embrapa 48: drought-tolerant). According to phylogenetic and domain analyses, these genes can be included in the DREB and ERF subfamilies. We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator. We detected the up-regulation of 9 GmAP2/EREB-like genes and identified transcriptional differences that were dependent on the levels of the stress applied and the tissue type analyzed (the expression of the GmDREB1F-like gene, for example, was four times higher in roots than in leaves). The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress. Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation.

Show MeSH
Related in: MedlinePlus