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Sexual dimorphism floral microRNA profiling and target gene expression in andromonoecious poplar (Populus tomentosa).

Song Y, Ma K, Ci D, Zhang Z, Zhang D - PLoS ONE (2013)

Bottom Line: The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome.Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX.Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, P. R. China.

ABSTRACT
Although the molecular basis of poplar sex-specific flower development remains largely unknown, increasing evidence indicates an essential role for microRNAs (miRNAs). The specific miRNA types and precise miRNA expression patterns in dioecious plant flower development remain unclear. Here, we used andromonoecious poplar, an exceptional model system, to eliminate the confounding effects of genetic background of dioecious plants. This system, combined with high-throughput sequencing and computational analysis, allowed us to characterize sex-specific miRNAomes from female and male flowers. Comparative miRNAome analysis combined with quantitative real-time PCR revealed the expression patterns of 27 miRNAs in poplar flower and showed that the targets of these miRNAs are involved in flower organogenesis, Ca(2+) transport, phytohormone synthesis and metabolism, and DNA methylation. This paper describes a complex regulatory network consisting of these miRNAs expressed in sex-specific flower development in a dioecious plant. The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome. Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX. Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions. To our knowledge, this report is the first systematic investigation of sex-specific flower-related miRNAs and their targets in poplar, and it deepens our understanding of the important regulatory functions of miRNAs in female and male flower development in this dioecious plant.

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A possible functional network of flower development related miRNAs in andromonoecious P. tomentosa.Black line represent repression of target gene expression.
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pone-0062681-g007: A possible functional network of flower development related miRNAs in andromonoecious P. tomentosa.Black line represent repression of target gene expression.

Mentions: The idea that miRNAs could play important roles in flower development has been suggested in previous studies. However, no systematic investigation has been conducted into how flower development may be regulated via miRNA-mediated processes. In the present study, a possible functional network, which connects flower development related miRNAs with their targets were first reported (Table 7 and Figure 7). Base on the gene functional annotation, all target genes were divided into four subgroups as shown in Figure 7.


Sexual dimorphism floral microRNA profiling and target gene expression in andromonoecious poplar (Populus tomentosa).

Song Y, Ma K, Ci D, Zhang Z, Zhang D - PLoS ONE (2013)

A possible functional network of flower development related miRNAs in andromonoecious P. tomentosa.Black line represent repression of target gene expression.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646847&req=5

pone-0062681-g007: A possible functional network of flower development related miRNAs in andromonoecious P. tomentosa.Black line represent repression of target gene expression.
Mentions: The idea that miRNAs could play important roles in flower development has been suggested in previous studies. However, no systematic investigation has been conducted into how flower development may be regulated via miRNA-mediated processes. In the present study, a possible functional network, which connects flower development related miRNAs with their targets were first reported (Table 7 and Figure 7). Base on the gene functional annotation, all target genes were divided into four subgroups as shown in Figure 7.

Bottom Line: The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome.Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX.Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, P. R. China.

ABSTRACT
Although the molecular basis of poplar sex-specific flower development remains largely unknown, increasing evidence indicates an essential role for microRNAs (miRNAs). The specific miRNA types and precise miRNA expression patterns in dioecious plant flower development remain unclear. Here, we used andromonoecious poplar, an exceptional model system, to eliminate the confounding effects of genetic background of dioecious plants. This system, combined with high-throughput sequencing and computational analysis, allowed us to characterize sex-specific miRNAomes from female and male flowers. Comparative miRNAome analysis combined with quantitative real-time PCR revealed the expression patterns of 27 miRNAs in poplar flower and showed that the targets of these miRNAs are involved in flower organogenesis, Ca(2+) transport, phytohormone synthesis and metabolism, and DNA methylation. This paper describes a complex regulatory network consisting of these miRNAs expressed in sex-specific flower development in a dioecious plant. The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome. Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX. Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions. To our knowledge, this report is the first systematic investigation of sex-specific flower-related miRNAs and their targets in poplar, and it deepens our understanding of the important regulatory functions of miRNAs in female and male flower development in this dioecious plant.

Show MeSH