Limits...
Sexual dimorphism floral microRNA profiling and target gene expression in andromonoecious poplar (Populus tomentosa).

Song Y, Ma K, Ci D, Zhang Z, Zhang D - PLoS ONE (2013)

Bottom Line: The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome.Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX.Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, P. R. China.

ABSTRACT
Although the molecular basis of poplar sex-specific flower development remains largely unknown, increasing evidence indicates an essential role for microRNAs (miRNAs). The specific miRNA types and precise miRNA expression patterns in dioecious plant flower development remain unclear. Here, we used andromonoecious poplar, an exceptional model system, to eliminate the confounding effects of genetic background of dioecious plants. This system, combined with high-throughput sequencing and computational analysis, allowed us to characterize sex-specific miRNAomes from female and male flowers. Comparative miRNAome analysis combined with quantitative real-time PCR revealed the expression patterns of 27 miRNAs in poplar flower and showed that the targets of these miRNAs are involved in flower organogenesis, Ca(2+) transport, phytohormone synthesis and metabolism, and DNA methylation. This paper describes a complex regulatory network consisting of these miRNAs expressed in sex-specific flower development in a dioecious plant. The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome. Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX. Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions. To our knowledge, this report is the first systematic investigation of sex-specific flower-related miRNAs and their targets in poplar, and it deepens our understanding of the important regulatory functions of miRNAs in female and male flower development in this dioecious plant.

Show MeSH
Distribution of novel miRNAs on chromosome XIX.A. 6 novel miRNAs and their targets located on chromosome XIX. B. Schematic diagram of the order of 6 novel miRNAs and their targets on chromosome XIX. Each miRNA and corresponding targets are marked the same color. Putative sex determination region in a 2.45-Mb region at the peritelomeric end of chromosome XIX, based on Yin et al (2008).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3646847&req=5

pone-0062681-g004: Distribution of novel miRNAs on chromosome XIX.A. 6 novel miRNAs and their targets located on chromosome XIX. B. Schematic diagram of the order of 6 novel miRNAs and their targets on chromosome XIX. Each miRNA and corresponding targets are marked the same color. Putative sex determination region in a 2.45-Mb region at the peritelomeric end of chromosome XIX, based on Yin et al (2008).

Mentions: It was reported that Chromosome XIX is an incipient sex chromosome in Populus[21], [22]. In the present study, 5 novel miRNAs located in chromosome XIX were identified, including Pto-F6, Pto-F70, Pto-F71, Pto-F72 and Pto-F73 (Table 6; Figure 4A). Pto-F6 was expressed both in female and male flower libraries, but expressed at higher levels in female flowers than in male (Table 3). The Pto-70, Pto-71, Pto-72 and Pto-73 female-specific miRNAs were weakly expressed in female flowers (Table 5). A total of 33 targets located on chromosome XIX were identified for these novel miRNAs. Among these, Pto-F6 and Pto-73 were predicted to match 14 targets respectively that are located on chromosome XIX (Table 6). By contrast, no targets were identified for Pto-F72. Putative functions of these targets genes were annotated by BLAST searches against the TAIR, NCBI and JGI protein datasets. The results revealed that target genes are involved in disease resistance, response to auxin stimulus, decreased DNA methylation and membrane transport. Yin et al (2008) indicated that a 2.45 Mb segment in the peritelomeric region of chromosome XIX and is associated with gender determination [21]. In our study, Pto-70 and 4 targets genes were found in sex-determination regions (Figure 4B). Among the 4 target genes, 3 targets were predicted to match Pto-73 miRNAs and one was predicted to match Pto-F6 miRNA.


Sexual dimorphism floral microRNA profiling and target gene expression in andromonoecious poplar (Populus tomentosa).

Song Y, Ma K, Ci D, Zhang Z, Zhang D - PLoS ONE (2013)

Distribution of novel miRNAs on chromosome XIX.A. 6 novel miRNAs and their targets located on chromosome XIX. B. Schematic diagram of the order of 6 novel miRNAs and their targets on chromosome XIX. Each miRNA and corresponding targets are marked the same color. Putative sex determination region in a 2.45-Mb region at the peritelomeric end of chromosome XIX, based on Yin et al (2008).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646847&req=5

pone-0062681-g004: Distribution of novel miRNAs on chromosome XIX.A. 6 novel miRNAs and their targets located on chromosome XIX. B. Schematic diagram of the order of 6 novel miRNAs and their targets on chromosome XIX. Each miRNA and corresponding targets are marked the same color. Putative sex determination region in a 2.45-Mb region at the peritelomeric end of chromosome XIX, based on Yin et al (2008).
Mentions: It was reported that Chromosome XIX is an incipient sex chromosome in Populus[21], [22]. In the present study, 5 novel miRNAs located in chromosome XIX were identified, including Pto-F6, Pto-F70, Pto-F71, Pto-F72 and Pto-F73 (Table 6; Figure 4A). Pto-F6 was expressed both in female and male flower libraries, but expressed at higher levels in female flowers than in male (Table 3). The Pto-70, Pto-71, Pto-72 and Pto-73 female-specific miRNAs were weakly expressed in female flowers (Table 5). A total of 33 targets located on chromosome XIX were identified for these novel miRNAs. Among these, Pto-F6 and Pto-73 were predicted to match 14 targets respectively that are located on chromosome XIX (Table 6). By contrast, no targets were identified for Pto-F72. Putative functions of these targets genes were annotated by BLAST searches against the TAIR, NCBI and JGI protein datasets. The results revealed that target genes are involved in disease resistance, response to auxin stimulus, decreased DNA methylation and membrane transport. Yin et al (2008) indicated that a 2.45 Mb segment in the peritelomeric region of chromosome XIX and is associated with gender determination [21]. In our study, Pto-70 and 4 targets genes were found in sex-determination regions (Figure 4B). Among the 4 target genes, 3 targets were predicted to match Pto-73 miRNAs and one was predicted to match Pto-F6 miRNA.

Bottom Line: The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome.Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX.Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, P. R. China.

ABSTRACT
Although the molecular basis of poplar sex-specific flower development remains largely unknown, increasing evidence indicates an essential role for microRNAs (miRNAs). The specific miRNA types and precise miRNA expression patterns in dioecious plant flower development remain unclear. Here, we used andromonoecious poplar, an exceptional model system, to eliminate the confounding effects of genetic background of dioecious plants. This system, combined with high-throughput sequencing and computational analysis, allowed us to characterize sex-specific miRNAomes from female and male flowers. Comparative miRNAome analysis combined with quantitative real-time PCR revealed the expression patterns of 27 miRNAs in poplar flower and showed that the targets of these miRNAs are involved in flower organogenesis, Ca(2+) transport, phytohormone synthesis and metabolism, and DNA methylation. This paper describes a complex regulatory network consisting of these miRNAs expressed in sex-specific flower development in a dioecious plant. The conserved and novel miRNA locations were annotated in the Populus trichocarpa genome. Among these, miRNA Pto-F70 and 4 targets are located in the sex-determination regions of chromosome XIX. Furthermore, two novel miRNAs, Pto-F47 and Pto-F68, were shown for the first time to be regulatory factors in phytohormone interactions. To our knowledge, this report is the first systematic investigation of sex-specific flower-related miRNAs and their targets in poplar, and it deepens our understanding of the important regulatory functions of miRNAs in female and male flower development in this dioecious plant.

Show MeSH