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A behavioral paradigm to evaluate hippocampal performance in aged rodents for pharmacological and genetic target validation.

Gerstein H, Hullinger R, Lindstrom MJ, Burger C - PLoS ONE (2013)

Bottom Line: This task, however, is not practical for pre- and post-pharmacological treatment, as the memory of the task is long lasting.In contrast, the object location memory task, also a spatial learning paradigm, results in a less robust memory that decays quickly.This method provides a tool to evaluate the effect of treatments on cognitive impairment associated with aging and neurodegenerative disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, University of Wisconsin-Madison, Medical Sciences Center, Madison, Wisconsin, United States of America.

ABSTRACT
Aged-related cognitive ability is highly variable, ranging from unimpaired to severe impairments. The Morris water maze (a reliable tool for assessing memory) has been used to distinguish aged rodents that are superior learners from those that are learning impaired. This task, however, is not practical for pre- and post-pharmacological treatment, as the memory of the task is long lasting. In contrast, the object location memory task, also a spatial learning paradigm, results in a less robust memory that decays quickly. We demonstrate for the first time how these two paradigms can be used together to assess hippocampal cognitive impairments before and after pharmacological or genetic manipulations in rodents. Rats were first segregated into superior learning and learning impaired groups using the object location memory task, and their performance was correlated with future outcome on this task and on the Morris water maze. This method provides a tool to evaluate the effect of treatments on cognitive impairment associated with aging and neurodegenerative disorders.

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Related in: MedlinePlus

The OLM paradigm features novel and familiar object locations.Overhead view of OLM arena. (A) Object locations on Training Day. (B, C) Object locations on Testing Day.
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pone-0062360-g001: The OLM paradigm features novel and familiar object locations.Overhead view of OLM arena. (A) Object locations on Training Day. (B, C) Object locations on Testing Day.

Mentions: On Training Day, 24 hours after Habituation, rats were trained on the locations of two identical objects. The arena was the same as previously described but with the addition of two identical objects (Duplo™ plastic blocks, 1.25×1.25×1 inch) in corner locations A and B (Fig. 1A), approximately 2.5 cm from the sides of the arena. The orientation of the box is such that the side featuring a spatial cue (X marked in tape) is East. Rats were allowed to explore the arena and the two objects freely over the course of a 10-minute trial. The bedding was stirred and the blocks were cleaned with 70% ethanol, before moving on to the next animal. Any animal failing to investigate both objects on the Training trial or whose total investigation time on the Training trial was less than 10 s, was excluded from the analysis so as to avoid confusing very low activity with low novelty-seeking behavior.


A behavioral paradigm to evaluate hippocampal performance in aged rodents for pharmacological and genetic target validation.

Gerstein H, Hullinger R, Lindstrom MJ, Burger C - PLoS ONE (2013)

The OLM paradigm features novel and familiar object locations.Overhead view of OLM arena. (A) Object locations on Training Day. (B, C) Object locations on Testing Day.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646843&req=5

pone-0062360-g001: The OLM paradigm features novel and familiar object locations.Overhead view of OLM arena. (A) Object locations on Training Day. (B, C) Object locations on Testing Day.
Mentions: On Training Day, 24 hours after Habituation, rats were trained on the locations of two identical objects. The arena was the same as previously described but with the addition of two identical objects (Duplo™ plastic blocks, 1.25×1.25×1 inch) in corner locations A and B (Fig. 1A), approximately 2.5 cm from the sides of the arena. The orientation of the box is such that the side featuring a spatial cue (X marked in tape) is East. Rats were allowed to explore the arena and the two objects freely over the course of a 10-minute trial. The bedding was stirred and the blocks were cleaned with 70% ethanol, before moving on to the next animal. Any animal failing to investigate both objects on the Training trial or whose total investigation time on the Training trial was less than 10 s, was excluded from the analysis so as to avoid confusing very low activity with low novelty-seeking behavior.

Bottom Line: This task, however, is not practical for pre- and post-pharmacological treatment, as the memory of the task is long lasting.In contrast, the object location memory task, also a spatial learning paradigm, results in a less robust memory that decays quickly.This method provides a tool to evaluate the effect of treatments on cognitive impairment associated with aging and neurodegenerative disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, University of Wisconsin-Madison, Medical Sciences Center, Madison, Wisconsin, United States of America.

ABSTRACT
Aged-related cognitive ability is highly variable, ranging from unimpaired to severe impairments. The Morris water maze (a reliable tool for assessing memory) has been used to distinguish aged rodents that are superior learners from those that are learning impaired. This task, however, is not practical for pre- and post-pharmacological treatment, as the memory of the task is long lasting. In contrast, the object location memory task, also a spatial learning paradigm, results in a less robust memory that decays quickly. We demonstrate for the first time how these two paradigms can be used together to assess hippocampal cognitive impairments before and after pharmacological or genetic manipulations in rodents. Rats were first segregated into superior learning and learning impaired groups using the object location memory task, and their performance was correlated with future outcome on this task and on the Morris water maze. This method provides a tool to evaluate the effect of treatments on cognitive impairment associated with aging and neurodegenerative disorders.

Show MeSH
Related in: MedlinePlus