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Two-gene phylogeny of bright-spored Myxomycetes (slime moulds, superorder Lucisporidia).

Fiore-Donno AM, Clissmann F, Meyer M, Schnittler M, Cavalier-Smith T - PLoS ONE (2013)

Bottom Line: We discuss possible evolutionary pathways.Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding.We report a new group I intron insertion site for Myxomycetes in one Licea.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, University of Oxford, Oxford, United Kingdom. afiore-donno6@infomaniak.ch

ABSTRACT
Myxomycetes, or plasmodial slime-moulds, are one of the largest groups in phylum Amoebozoa. Nonetheless, only ∼10% are in the database for the small subunit (SSU) ribosomal RNA gene, the most widely used gene for phylogenetics and barcoding. Most sequences belong to dark-spored Myxomycetes (order Fuscisporida); the 318 species of superorder Lucisporidia (bright-spored) are represented by only eleven genuine sequences. To compensate for this, we provide 66 new sequences, 37 SSU rRNA and 29 elongation factor 1-alpha (EF-1α), for 82% of the genera of Lucisporidia. Phylogenetic analyses of single- and two-gene alignments produce congruent topologies and reveal both morphological characters that have been overemphasised and those that have been overlooked in past classifications. Both classical orders, Liceida and Trichiida, and several families and genera are para/polyphyletic; some previously unrecognised clades emerge. We discuss possible evolutionary pathways. Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding. We report a new group I intron insertion site for Myxomycetes in one Licea.

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Dianema nivale, vertical section with the spore mass partially blown away, showing the capillitium connecting the peridium with the base of the fructification.Scale and colours are approximate. Credit photos: Michel Poulain.
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pone-0062586-g005: Dianema nivale, vertical section with the spore mass partially blown away, showing the capillitium connecting the peridium with the base of the fructification.Scale and colours are approximate. Credit photos: Michel Poulain.

Mentions: In new clade 1, Dictydiaethaliidae are associated with Dianemidae, Prototrichia metallica (with hollow capillitium) and Licea variabilis (lacking capillitium). A capillitium connecting the peridium to the base of the sporotheca is a characteristic common to Dianemidae and Prototrichia metallica (Fig. 5). Interestingly, a scanning electron microscopic study of several Trichiida recognized five groups of capillitium, the first three of which are found in new clade 1: Calomyxa metallica (Type I), Dianema (Type II), Prototricha metallica (Type III) [40]. This pattern supports the phylogenetic relationships of these species within new clade I (Fig. 1, Fig. 2). The question arises whether the capillitial Type I, II and III could be homologuous to the “peridial threads” of Dictydiaethalium. Should this be true, the fruiting bodies of Licea variabilis, Prototrichia metallica, Calomyxa metallica and Dianema spp. would be reduced aethalia (Fig. 2).


Two-gene phylogeny of bright-spored Myxomycetes (slime moulds, superorder Lucisporidia).

Fiore-Donno AM, Clissmann F, Meyer M, Schnittler M, Cavalier-Smith T - PLoS ONE (2013)

Dianema nivale, vertical section with the spore mass partially blown away, showing the capillitium connecting the peridium with the base of the fructification.Scale and colours are approximate. Credit photos: Michel Poulain.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646832&req=5

pone-0062586-g005: Dianema nivale, vertical section with the spore mass partially blown away, showing the capillitium connecting the peridium with the base of the fructification.Scale and colours are approximate. Credit photos: Michel Poulain.
Mentions: In new clade 1, Dictydiaethaliidae are associated with Dianemidae, Prototrichia metallica (with hollow capillitium) and Licea variabilis (lacking capillitium). A capillitium connecting the peridium to the base of the sporotheca is a characteristic common to Dianemidae and Prototrichia metallica (Fig. 5). Interestingly, a scanning electron microscopic study of several Trichiida recognized five groups of capillitium, the first three of which are found in new clade 1: Calomyxa metallica (Type I), Dianema (Type II), Prototricha metallica (Type III) [40]. This pattern supports the phylogenetic relationships of these species within new clade I (Fig. 1, Fig. 2). The question arises whether the capillitial Type I, II and III could be homologuous to the “peridial threads” of Dictydiaethalium. Should this be true, the fruiting bodies of Licea variabilis, Prototrichia metallica, Calomyxa metallica and Dianema spp. would be reduced aethalia (Fig. 2).

Bottom Line: We discuss possible evolutionary pathways.Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding.We report a new group I intron insertion site for Myxomycetes in one Licea.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, University of Oxford, Oxford, United Kingdom. afiore-donno6@infomaniak.ch

ABSTRACT
Myxomycetes, or plasmodial slime-moulds, are one of the largest groups in phylum Amoebozoa. Nonetheless, only ∼10% are in the database for the small subunit (SSU) ribosomal RNA gene, the most widely used gene for phylogenetics and barcoding. Most sequences belong to dark-spored Myxomycetes (order Fuscisporida); the 318 species of superorder Lucisporidia (bright-spored) are represented by only eleven genuine sequences. To compensate for this, we provide 66 new sequences, 37 SSU rRNA and 29 elongation factor 1-alpha (EF-1α), for 82% of the genera of Lucisporidia. Phylogenetic analyses of single- and two-gene alignments produce congruent topologies and reveal both morphological characters that have been overemphasised and those that have been overlooked in past classifications. Both classical orders, Liceida and Trichiida, and several families and genera are para/polyphyletic; some previously unrecognised clades emerge. We discuss possible evolutionary pathways. Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding. We report a new group I intron insertion site for Myxomycetes in one Licea.

Show MeSH