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Two-gene phylogeny of bright-spored Myxomycetes (slime moulds, superorder Lucisporidia).

Fiore-Donno AM, Clissmann F, Meyer M, Schnittler M, Cavalier-Smith T - PLoS ONE (2013)

Bottom Line: We discuss possible evolutionary pathways.Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding.We report a new group I intron insertion site for Myxomycetes in one Licea.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, University of Oxford, Oxford, United Kingdom. afiore-donno6@infomaniak.ch

ABSTRACT
Myxomycetes, or plasmodial slime-moulds, are one of the largest groups in phylum Amoebozoa. Nonetheless, only ∼10% are in the database for the small subunit (SSU) ribosomal RNA gene, the most widely used gene for phylogenetics and barcoding. Most sequences belong to dark-spored Myxomycetes (order Fuscisporida); the 318 species of superorder Lucisporidia (bright-spored) are represented by only eleven genuine sequences. To compensate for this, we provide 66 new sequences, 37 SSU rRNA and 29 elongation factor 1-alpha (EF-1α), for 82% of the genera of Lucisporidia. Phylogenetic analyses of single- and two-gene alignments produce congruent topologies and reveal both morphological characters that have been overemphasised and those that have been overlooked in past classifications. Both classical orders, Liceida and Trichiida, and several families and genera are para/polyphyletic; some previously unrecognised clades emerge. We discuss possible evolutionary pathways. Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding. We report a new group I intron insertion site for Myxomycetes in one Licea.

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Pseudoaethalia and aethalia in Reticulariidae.A.Tubifera ferruginosa, pseudoaethalium seen from above. B. Vertical section showing sporangia surrounded by peridia; note the lack of capillitium in the spore mass. C.Lycogala epidendrum, aethalia seen from above. D. Vertical section of the same, with the spore mass blown away and the abundant pseudocapillitium. Scales and colours are approximate. Credit photos: Michel Poulain.
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pone-0062586-g003: Pseudoaethalia and aethalia in Reticulariidae.A.Tubifera ferruginosa, pseudoaethalium seen from above. B. Vertical section showing sporangia surrounded by peridia; note the lack of capillitium in the spore mass. C.Lycogala epidendrum, aethalia seen from above. D. Vertical section of the same, with the spore mass blown away and the abundant pseudocapillitium. Scales and colours are approximate. Credit photos: Michel Poulain.

Mentions: In Reticulariidae an interesting pattern, distinguishing pseudoaethalia from aethalia is observed. In one clade, consisting of Tubifera ferruginosa and T. dimorphotheca, the fruiting bodies are composed of closely compressed sporangia, retaining their peridium (pseudoaethalia) (Fig. 3 A, B). In the other clade, true aethalia are formed, as in Reticularia jurana, R. lycoperdon and Lycogala epidendrum, with fruiting body appearing as a large, single mass, where the individual sporangia cannot be distinguished (Fig. 3 C, D).


Two-gene phylogeny of bright-spored Myxomycetes (slime moulds, superorder Lucisporidia).

Fiore-Donno AM, Clissmann F, Meyer M, Schnittler M, Cavalier-Smith T - PLoS ONE (2013)

Pseudoaethalia and aethalia in Reticulariidae.A.Tubifera ferruginosa, pseudoaethalium seen from above. B. Vertical section showing sporangia surrounded by peridia; note the lack of capillitium in the spore mass. C.Lycogala epidendrum, aethalia seen from above. D. Vertical section of the same, with the spore mass blown away and the abundant pseudocapillitium. Scales and colours are approximate. Credit photos: Michel Poulain.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646832&req=5

pone-0062586-g003: Pseudoaethalia and aethalia in Reticulariidae.A.Tubifera ferruginosa, pseudoaethalium seen from above. B. Vertical section showing sporangia surrounded by peridia; note the lack of capillitium in the spore mass. C.Lycogala epidendrum, aethalia seen from above. D. Vertical section of the same, with the spore mass blown away and the abundant pseudocapillitium. Scales and colours are approximate. Credit photos: Michel Poulain.
Mentions: In Reticulariidae an interesting pattern, distinguishing pseudoaethalia from aethalia is observed. In one clade, consisting of Tubifera ferruginosa and T. dimorphotheca, the fruiting bodies are composed of closely compressed sporangia, retaining their peridium (pseudoaethalia) (Fig. 3 A, B). In the other clade, true aethalia are formed, as in Reticularia jurana, R. lycoperdon and Lycogala epidendrum, with fruiting body appearing as a large, single mass, where the individual sporangia cannot be distinguished (Fig. 3 C, D).

Bottom Line: We discuss possible evolutionary pathways.Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding.We report a new group I intron insertion site for Myxomycetes in one Licea.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, University of Oxford, Oxford, United Kingdom. afiore-donno6@infomaniak.ch

ABSTRACT
Myxomycetes, or plasmodial slime-moulds, are one of the largest groups in phylum Amoebozoa. Nonetheless, only ∼10% are in the database for the small subunit (SSU) ribosomal RNA gene, the most widely used gene for phylogenetics and barcoding. Most sequences belong to dark-spored Myxomycetes (order Fuscisporida); the 318 species of superorder Lucisporidia (bright-spored) are represented by only eleven genuine sequences. To compensate for this, we provide 66 new sequences, 37 SSU rRNA and 29 elongation factor 1-alpha (EF-1α), for 82% of the genera of Lucisporidia. Phylogenetic analyses of single- and two-gene alignments produce congruent topologies and reveal both morphological characters that have been overemphasised and those that have been overlooked in past classifications. Both classical orders, Liceida and Trichiida, and several families and genera are para/polyphyletic; some previously unrecognised clades emerge. We discuss possible evolutionary pathways. Our study fills a gap in the phylogeny of Amoebozoa and provides an extensive SSU rRNA sequence reference database for environmental sampling and barcoding. We report a new group I intron insertion site for Myxomycetes in one Licea.

Show MeSH