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Comparison of rapid diagnostic tests for the detection of Plasmodium vivax malaria in South Korea.

Kim JY, Ji SY, Goo YK, Na BK, Pyo HJ, Lee HN, Lee J, Kim NH, von Seidlein L, Cheng Q, Cho SH, Lee WJ - PLoS ONE (2013)

Bottom Line: The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively).The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples.Lower test price would result in increased use of these RDTs in the field.

View Article: PubMed Central - PubMed

Affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong, Republic of Korea.

ABSTRACT
South Korea is one of many countries with endemic Plasmodium vivax malaria. Here we report the evaluation of four rapid diagnostic tests (RDTs) for diagnosis of this disease. A total of 253 subjects were enrolled in the study. The sensitivities, specificities and agreement frequencies were estimated by comparing the four RDTs against the standard of nested-PCR and microscopic examination. The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively). The CareStart(TM) and SD Bioline tests could detect P. vivax in samples with parasite densities <150/μl, which was a slightly better performance than the other two RDTs. The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples. Lower test price would result in increased use of these RDTs in the field. The results of this study contribute valuable information that will aid in the selection of a diagnostic method for the detection of malaria.

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Related in: MedlinePlus

Correlation between intensity of the positive band of four rapid diagnostic tests (CareStartTM, SD Bioline, NanoSign, and Asan Easy) and parasitemia (determined by count of thick blood smears).***; If the strength of positive line is greater than that of control line (Control line < Positive line), **; If the strength of the positive line is similar to the control line (Control line  =  Positive line), *; If the strength of the positive line is less than the control line (Control line > Positive line).
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pone-0064353-g002: Correlation between intensity of the positive band of four rapid diagnostic tests (CareStartTM, SD Bioline, NanoSign, and Asan Easy) and parasitemia (determined by count of thick blood smears).***; If the strength of positive line is greater than that of control line (Control line < Positive line), **; If the strength of the positive line is similar to the control line (Control line  =  Positive line), *; If the strength of the positive line is less than the control line (Control line > Positive line).

Mentions: To determine whether the RDTs could detect low numbers of P. vivax, results for each sample were categorized according to parasite density. The 62 samples that were shipped with blood smears slides or prepared within 6 hours after blood collection were used for this evaluation. CareStartTM and SD Bioline tests could detect P. vivax at low parasite densities (sensitivity: 100%), the NanoSign test had a low sensitivity (60%) at a parasite density of 1–150/μl. We wondered whether we could use RDTs to quantify the parasite load in the patient by comparing the ratio of test to control band intensity. Therefore, the ratio of test to control band intensity for the four RDTs was compared to corresponding parasitemia. There was a slight positive correlation between the ratio of the test to control band density for each RDT and the parasitemia counts (Fig. 2).


Comparison of rapid diagnostic tests for the detection of Plasmodium vivax malaria in South Korea.

Kim JY, Ji SY, Goo YK, Na BK, Pyo HJ, Lee HN, Lee J, Kim NH, von Seidlein L, Cheng Q, Cho SH, Lee WJ - PLoS ONE (2013)

Correlation between intensity of the positive band of four rapid diagnostic tests (CareStartTM, SD Bioline, NanoSign, and Asan Easy) and parasitemia (determined by count of thick blood smears).***; If the strength of positive line is greater than that of control line (Control line < Positive line), **; If the strength of the positive line is similar to the control line (Control line  =  Positive line), *; If the strength of the positive line is less than the control line (Control line > Positive line).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646812&req=5

pone-0064353-g002: Correlation between intensity of the positive band of four rapid diagnostic tests (CareStartTM, SD Bioline, NanoSign, and Asan Easy) and parasitemia (determined by count of thick blood smears).***; If the strength of positive line is greater than that of control line (Control line < Positive line), **; If the strength of the positive line is similar to the control line (Control line  =  Positive line), *; If the strength of the positive line is less than the control line (Control line > Positive line).
Mentions: To determine whether the RDTs could detect low numbers of P. vivax, results for each sample were categorized according to parasite density. The 62 samples that were shipped with blood smears slides or prepared within 6 hours after blood collection were used for this evaluation. CareStartTM and SD Bioline tests could detect P. vivax at low parasite densities (sensitivity: 100%), the NanoSign test had a low sensitivity (60%) at a parasite density of 1–150/μl. We wondered whether we could use RDTs to quantify the parasite load in the patient by comparing the ratio of test to control band intensity. Therefore, the ratio of test to control band intensity for the four RDTs was compared to corresponding parasitemia. There was a slight positive correlation between the ratio of the test to control band density for each RDT and the parasitemia counts (Fig. 2).

Bottom Line: The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively).The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples.Lower test price would result in increased use of these RDTs in the field.

View Article: PubMed Central - PubMed

Affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong, Republic of Korea.

ABSTRACT
South Korea is one of many countries with endemic Plasmodium vivax malaria. Here we report the evaluation of four rapid diagnostic tests (RDTs) for diagnosis of this disease. A total of 253 subjects were enrolled in the study. The sensitivities, specificities and agreement frequencies were estimated by comparing the four RDTs against the standard of nested-PCR and microscopic examination. The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively). The CareStart(TM) and SD Bioline tests could detect P. vivax in samples with parasite densities <150/μl, which was a slightly better performance than the other two RDTs. The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples. Lower test price would result in increased use of these RDTs in the field. The results of this study contribute valuable information that will aid in the selection of a diagnostic method for the detection of malaria.

Show MeSH
Related in: MedlinePlus